##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbrPI BlnI BsaBI BsiWI
BsmBI BspHI BspLU11I BssSI BstAPI
BstBI BstEII BstPI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SfiI
SfuI SgrAI SmaI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 1428 CTTAAG C^TTAAG
ApaI 278 GGGCCC GGGCC^C
Asp700I 2255 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 231 CCCGAG C^[CT]CG[GA]G
BalI 920 TGGCCA TGG^CCA
BbsI 1115 GAAGAC GAAGAC(2/6)
BcgI 181 GCAGCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3110 GTATCC GTATCC(6/5)
BclI 712 TGATCA T^GATCA
BfrI 1428 CTTAAG C^TTAAG
BglII 3065 AGATCT A^GATCT
BlpI 35 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 35 GCTAAGC GC^T[ACGT]AGC
BpuAI 1115 GAAGAC GAAGAC(2/6)
BsaAI 3107 TACGTA [CT]AC^GT[GA]
BsgI 2160 GTGCAG GTGCAG(16/14)
BsmI 2518 GAATGC GAATGC(1/-1)
BsoBI 231 CCCGAG C^[CT]CG[GA]G
BspDI 1097 ATCGAT AT^CGAT
BsrFI 166 GCCGGC [GA]^CCGG[CT]
BssHII 122 GCGCGC G^CGCGC
Bst1107I 1794 GTATAC GTA^TAC
BstZ17I 1794 GTATAC GTA^TAC
CelII 35 GCTAAGC GC^T[ACGT]AGC
Cfr10I 166 GCCGGC [GA]^CCGG[CT]
ClaI 1097 ATCGAT AT^CGAT
DraII 3294 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 388 CGGCCG C^GGCCG
EarI 2818 CTCTTC CTCTTC(1/4)
EclXI 388 CGGCCG C^GGCCG
Eco52I 388 CGGCCG C^GGCCG
EcoO109I 3294 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 3493 GAATTC G^AATTC
EcoRV 528 GATATC GAT^ATC
FbaI 712 TGATCA T^GATCA
Ksp632I 2818 CTCTTC CTCTTC(1/4)
MluNI 920 TGGCCA TGG^CCA
MscI 920 TGGCCA TGG^CCA
NaeI 166 GCCGGC GCC^GGC
NgoMIV 166 GCCGGC G^CCGGC
PflMI 1287 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3294 AGGTCCT [GA]G^G[AT]CC[CT]
SalI 465 GTCGAC G^TCGAC
SexAI 565 ACCTGGT A^CC[AT]GGT
SnaBI 3107 TACGTA TAC^GTA
Van91I 1287 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2255 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 465 GTCGAC GT^[AC][GT]AC
1794 GTATAC
AccIII 613 TCCGGA T^CCGGA
1436 TCCGGA
AspI 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACGTTGTC
BamHI 1433 GGATCC G^GATCC
2419 GGATCC
BpmI 2694 CTCCAG CTGGAG(16/14)
1140 CTGGAG
BsaI 1391 GAGACC GGTCTC(1/5)
2594 GAGACC
BseAI 613 TCCGGA T^CCGGA
1436 TCCGGA
BsiEI 265 CGACCG CG[GA][CT]^CG
388 CGGCCG
BspEI 613 TCCGGA T^CCGGA
1436 TCCGGA
DraI 1862 TTTAAA TTT^AAA
2323 TTTAAA
DrdI 1047 GACATCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3647 GACATCTGTGTC
HgaI 332 GACGC GACGC(5/10)
411 GACGC
HindIII 1537 AAGCTT A^AGCTT
2471 AAGCTT
MroI 613 TCCGGA T^CCGGA
1436 TCCGGA
NcoI 786 CCATGG C^CATGG
2952 CCATGG
PflFI 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACGTTGTC
PstI 222 CTGCAG CTGCA^G
1018 CTGCAG
SacI 558 GAGCTC GAGCT^C
2201 GAGCTC
SpeI 1334 ACTAGT A^CTAGT
3079 ACTAGT
Tth111I 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3705 GACGTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI Eco81I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SphI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII