################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BlnI BsaBI BsiWI BsmBI BspHI BspLU11I BssSI BstAPI BstBI BstEII BstPI CpoI DraIII Eam1105I Eco47III EcoNI EcoO65I FseI FspI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SfiI SfuI SgrAI SmaI Sse8387I SspI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflII 1428 CTTAAG C^TTAAG ApaI 278 GGGCCC GGGCC^C Asp700I 2255 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 231 CCCGAG C^[CT]CG[GA]G BalI 920 TGGCCA TGG^CCA BbsI 1115 GAAGAC GAAGAC(2/6) BcgI 181 GCAGCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3110 GTATCC GTATCC(6/5) BclI 712 TGATCA T^GATCA BfrI 1428 CTTAAG C^TTAAG BglII 3065 AGATCT A^GATCT BlpI 35 GCTAAGC GC^T[ACGT]AGC Bpu1102I 35 GCTAAGC GC^T[ACGT]AGC BpuAI 1115 GAAGAC GAAGAC(2/6) BsaAI 3107 TACGTA [CT]AC^GT[GA] BsgI 2160 GTGCAG GTGCAG(16/14) BsmI 2518 GAATGC GAATGC(1/-1) BsoBI 231 CCCGAG C^[CT]CG[GA]G BspDI 1097 ATCGAT AT^CGAT BsrFI 166 GCCGGC [GA]^CCGG[CT] BssHII 122 GCGCGC G^CGCGC Bst1107I 1794 GTATAC GTA^TAC BstZ17I 1794 GTATAC GTA^TAC CelII 35 GCTAAGC GC^T[ACGT]AGC Cfr10I 166 GCCGGC [GA]^CCGG[CT] ClaI 1097 ATCGAT AT^CGAT DraII 3294 AGGTCCT [GA]G^G[ACGT]CC[CT] EagI 388 CGGCCG C^GGCCG EarI 2818 CTCTTC CTCTTC(1/4) EclXI 388 CGGCCG C^GGCCG Eco52I 388 CGGCCG C^GGCCG EcoO109I 3294 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 3493 GAATTC G^AATTC EcoRV 528 GATATC GAT^ATC FbaI 712 TGATCA T^GATCA Ksp632I 2818 CTCTTC CTCTTC(1/4) MluNI 920 TGGCCA TGG^CCA MscI 920 TGGCCA TGG^CCA NaeI 166 GCCGGC GCC^GGC NgoMIV 166 GCCGGC G^CCGGC PflMI 1287 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3294 AGGTCCT [GA]G^G[AT]CC[CT] SalI 465 GTCGAC G^TCGAC SexAI 565 ACCTGGT A^CC[AT]GGT SnaBI 3107 TACGTA TAC^GTA Van91I 1287 CCACTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2255 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 465 GTCGAC GT^[AC][GT]AC 1794 GTATAC AccIII 613 TCCGGA T^CCGGA 1436 TCCGGA AspI 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3705 GACGTTGTC BamHI 1433 GGATCC G^GATCC 2419 GGATCC BpmI 2694 CTCCAG CTGGAG(16/14) 1140 CTGGAG BsaI 1391 GAGACC GGTCTC(1/5) 2594 GAGACC BseAI 613 TCCGGA T^CCGGA 1436 TCCGGA BsiEI 265 CGACCG CG[GA][CT]^CG 388 CGGCCG BspEI 613 TCCGGA T^CCGGA 1436 TCCGGA DraI 1862 TTTAAA TTT^AAA 2323 TTTAAA DrdI 1047 GACATCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3647 GACATCTGTGTC HgaI 332 GACGC GACGC(5/10) 411 GACGC HindIII 1537 AAGCTT A^AGCTT 2471 AAGCTT MroI 613 TCCGGA T^CCGGA 1436 TCCGGA NcoI 786 CCATGG C^CATGG 2952 CCATGG PflFI 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3705 GACGTTGTC PstI 222 CTGCAG CTGCA^G 1018 CTGCAG SacI 558 GAGCTC GAGCT^C 2201 GAGCTC SpeI 1334 ACTAGT A^CTAGT 3079 ACTAGT Tth111I 332 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 3705 GACGTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI Eco81I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII