##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AclI AflII AhdI
Aor51HI AseI AsnI Asp718I AspEI
AspI AviII BamHI BclI BfrI
BsiWI BspDI BspHI BspLU11I BsrBI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MluI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2055 GACGTC GACGT^C
AccIII 919 TCCGGA T^CCGGA
AflIII 825 ACGTGT A^C[GA][CT]GT
AgeI 3555 ACCGGT A^CCGGT
Alw44I 1471 GTGCAC G^TGCAC
AocI 95 CCTGAGG CC^T[ACGT]AGG
ApaLI 1471 GTGCAC G^TGCAC
AscI 2223 GGCGCGCC GG^CGCGCC
Asp700I 1631 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2207 CCTAGG C^CTAGG
BalI 799 TGGCCA TGG^CCA
BbeI 2903 GGCGCC GGCGC^C
BciVI 3352 GTATCC GTATCC(6/5)
BglII 3931 AGATCT A^GATCT
BlnI 2207 CCTAGG C^CTAGG
BsaBI 2008 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 919 TCCGGA T^CCGGA
BsmI 3964 GAATGC GAATGC(1/-1)
BspEI 919 TCCGGA T^CCGGA
BsrDI 1255 GCAATG GCAATG(2/0)
BssHII 2224 GCGCGC G^CGCGC
Bsu36I 95 CCTGAGG CC^T[ACGT]AGG
DraIII 407 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2782 GACCTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 95 CCTGAGG CC^T[ACGT]AGG
EcoRV 1073 GATATC GAT^ATC
HincII 1107 GTCAAC GT[CT]^[GA]AC
HindII 1107 GTCAAC GT[CT]^[GA]AC
KasI 2903 GGCGCC G^GCGCC
KspI 1209 CCGCGG CCGC^GG
MamI 2008 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 799 TGGCCA TGG^CCA
MroI 919 TCCGGA T^CCGGA
MscI 799 TGGCCA TGG^CCA
MslI 3059 CACGCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2030 GCCGGC GCC^GGC
NarI 2903 GGCGCC GG^CGCC
NgoMIV 2030 GCCGGC G^CCGGC
NspI 844 GCATGT [GA]CATG^[CT]
PaeR7I 1762 CTCGAG C^TCGAG
PinAI 3555 ACCGGT A^CCGGT
PmeI 1519 GTTTAAAC GTTT^AAAC
PshAI 2282 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1209 CCGCGG CCGC^GG
SfiI 1266 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2903 GGCGCC GGC^GCC
SgrAI 3554 CACCGGTG C[GA]^CCGG[CT]G
TfiI 129 GATTC G^A[AT]TC
XcmI 3203 CCAGGGATCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1762 CTCGAG C^TCGAG
XmnI 1631 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 3642 GAATTT [GA]^AATT[CT]
3991 AAATTT
ApoI 3642 GAATTT [GA]^AATT[CT]
3991 AAATTT
BbrPI 72 CACGTG CAC^GTG
824 CACGTG
BbsI 2200 GAAGAC GAAGAC(2/6)
3903 GTCTTC
BlpI 720 GCTGAGC GC^T[ACGT]AGC
1095 GCTCAGC
Bpu1102I 720 GCTGAGC GC^T[ACGT]AGC
1095 GCTCAGC
BpuAI 2200 GAAGAC GAAGAC(2/6)
3903 GTCTTC
BsaAI 72 CACGTG [CT]AC^GT[GA]
824 CACGTG
BsaWI 919 TCCGGA [AT]^CCGG[AT]
3555 ACCGGT
BsmBI 471 GAGACG CGTCTC(1/5)
1566 GAGACG
BsrFI 2030 GCCGGC [GA]^CCGG[CT]
3555 ACCGGT
BstYI 1939 GGATCT [GA]^GATC[CT]
3931 AGATCT
CelII 720 GCTGAGC GC^T[ACGT]AGC
1095 GCTCAGC
Cfr10I 2030 GCCGGC [GA]^CCGG[CT]
3555 ACCGGT
DraI 1520 TTTAAA TTT^AAA
1985 TTTAAA
EarI 1604 CTCTTC CTCTTC(1/4)
620 GAAGAG
Ksp632I 1604 CTCTTC CTCTTC(1/4)
620 GAAGAG
MfeI 3004 CAATTG C^AATTG
3881 CAATTG
MflI 1939 GGATCT [GA]^GATC[CT]
3931 AGATCT
MunI 3004 CAATTG C^AATTG
3881 CAATTG
PmaCI 72 CACGTG CAC^GTG
824 CACGTG
PmlI 72 CACGTG CAC^GTG
824 CACGTG
SmlI 1762 CTCGAG C^T[CT][GA]AG
2284 CTTGAG
XhoII 1939 GGATCT [GA]^GATC[CT]
3931 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvCI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SspBI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmaCI
XmaI