##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AviII
BamHI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I FbaI FseI FspI
HpaI KpnI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AclI 3826 AACGTT AA^CGTT
AflIII 1130 ACATGT A^C[GA][CT]GT
AlwNI 2551 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2769 CCTGAGG CC^T[ACGT]AGG
AspHI 256 GAGCTC G[AT]GC[AT]^C
AspI 536 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2989 CCCGAG C^[CT]CG[GA]G
AvrII 27 CCTAGG C^CTAGG
BalI 699 TGGCCA TGG^CCA
BbeI 635 GGCGCC GGCGC^C
BciVI 2750 GGATAC GTATCC(6/5)
BlnI 27 CCTAGG C^CTAGG
BsaBI 2259 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 256 GAGCTC G[AT]GC[AT]^C
BsmI 2975 GCATTC GAATGC(1/-1)
BsoBI 2989 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3946 TGTACA T^GTACA
BspLU11I 1130 ACATGT A^CATGT
BspMI 1237 ACCTGC ACCTGC(4/8)
BsrDI 3003 CATTGC GCAATG(2/0)
BsrGI 3946 TGTACA T^GTACA
BstYI 1854 GGATCT [GA]^GATC[CT]
Bsu36I 2769 CCTGAGG CC^T[ACGT]AGG
CfoI 636 GCGC GCG^C
DraII 1874 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 326 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2769 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1874 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2973 ATGCAT ATGCA^T
HaeII 635 GGCGCC [GA]GCGC^[CT]
HhaI 636 GCGC GCG^C
HinP1I 636 GCGC G^CGC
HincII 1521 GTTGAC GT[CT]^[GA]AC
HindII 1521 GTTGAC GT[CT]^[GA]AC
KasI 635 GGCGCC G^GCGCC
KspI 96 CCGCGG CCGC^GG
MamI 2259 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1854 GGATCT [GA]^GATC[CT]
MluNI 699 TGGCCA TGG^CCA
MscI 699 TGGCCA TGG^CCA
NarI 635 GGCGCC GG^CGCC
NcoI 1734 CCATGG C^CATGG
NdeI 2019 CATATG CA^TATG
NheI 3429 GCTAGC G^CTAGC
NsiI 2973 ATGCAT ATGCA^T
PflFI 536 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 691 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1874 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 3826 AACGTT AA^CGTT
SacI 256 GAGCTC GAGCT^C
SacII 96 CCGCGG CCGC^GG
SapI 52 GCTCTTC GCTCTTC(1/4)
SfoI 635 GGCGCC GGC^GCC
SpeI 1058 ACTAGT A^CTAGT
SspBI 3946 TGTACA T^GTACA
Tth111I 536 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 691 CCATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2618 CCAAGCTATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1854 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 635 GGCGCC G[GA]^CG[CT]C
1557 GGCGTC
AseI 1865 ATTAAT AT^TAAT
1950 ATTAAT
AsnI 1865 ATTAAT AT^TAAT
1950 ATTAAT
AvaII 1875 GGTCC G^G[AT]CC
2615 GGTCC
BsaHI 635 GGCGCC G[GA]^CG[CT]C
1557 GGCGTC
BsgI 492 CTGCAC GTGCAG(16/14)
224 GTGCAG
BsrBI 50 CCGCTC CCGCTC(-3/-3)
3749 GAGCGG
Bst1107I 862 GTATAC GTA^TAC
988 GTATAC
BstZ17I 862 GTATAC GTA^TAC
988 GTATAC
DraI 428 TTTAAA TTT^AAA
2543 TTTAAA
EaeI 699 TGGCCA [CT]^GGCC[GA]
2520 CGGCCA
EcoRI 1052 GAATTC G^AATTC
1193 GAATTC
EcoRV 2460 GATATC GAT^ATC
2898 GATATC
Hin1I 635 GGCGCC G[GA]^CG[CT]C
1557 GGCGTC
HindIII 154 AAGCTT A^AGCTT
2012 AAGCTT
MslI 1337 CACATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3173 CATTCCAATG
NspI 1130 ACATGT [GA]CATG^[CT]
2794 ACATGC
PshAI 131 GACGCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
483 GACAACAGTC
PshBI 1865 ATTAAT AT^TAAT
1950 ATTAAT
PstI 239 CTGCAG CTGCA^G
3337 CTGCAG
SexAI 1809 ACCTGGT A^CC[AT]GGT
3384 ACCTGGT
SspI 300 AATATT AAT^ATT
2316 AATATT
XbaI 1544 TCTAGA T^CTAGA
2429 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I