##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AclI AflII Aor51HI AscI
AseI AsnI AvrII BamHI BfrI
BlnI BseAI BsiWI BsmI BspDI
BspEI BspLU11I BsrDI BstAPI BstBI
BstEII BstPI ClaI CpoI DraI
DrdI Eco47III EcoO65I EcoRI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SfuI SnaBI SpeI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 166 GACGTC GACGT^C
AcsI 3441 GAATTT [GA]^AATT[CT]
AgeI 3113 ACCGGT A^CCGGT
ApoI 3441 GAATTT [GA]^AATT[CT]
Asp700I 2701 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 2371 GCTGAGG CCTCAGC(-5/-2)
BcgI 1047 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3431 GTATCC GTATCC(6/5)
BclI 2280 TGATCA T^GATCA
BglII 1133 AGATCT A^GATCT
BsaBI 2848 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1663 CTGCAC GTGCAG(16/14)
Bsp1407I 3499 TGTACA T^GTACA
BspHI 1416 TCATGA T^CATGA
BsrGI 3499 TGTACA T^GTACA
Bst1107I 1066 GTATAC GTA^TAC
BstZ17I 1066 GTATAC GTA^TAC
EcoRV 2426 GATATC GAT^ATC
EcoT22I 3300 ATGCAT ATGCA^T
FbaI 2280 TGATCA T^GATCA
FseI 4133 GGCCGGCC GGCCGG^CC
HindIII 2233 AAGCTT A^AGCTT
HpaI 2921 GTTAAC GTT^AAC
MamI 2848 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2922 TTAA T^TAA
NcoI 3067 CCATGG C^CATGG
NdeI 2471 CATATG CA^TATG
NsiI 3300 ATGCAT ATGCA^T
PinAI 3113 ACCGGT A^CCGGT
RcaI 1416 TCATGA T^CATGA
SgrAI 3112 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3499 TGTACA T^GTACA
Tru9I 2922 TTAA T^TAA
XmnI 2701 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1530 GGTACC G^GTACC
3226 GGTACC
AhdI 194 GACGTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
910 GACCAACTGTC
AlwI 1564 GATCC GGATC(4/5)
2838 GGATC
Asp718I 1530 GGTACC G^GTACC
3226 GGTACC
AspEI 194 GACGTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
910 GACCAACTGTC
AspI 1643 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACCCTGTC
AviII 725 TGCGCA TGC^GCA
2643 TGCGCA
BbrPI 2069 CACGTG CAC^GTG
3286 CACGTG
BsaI 3344 GAGACC GGTCTC(1/5)
4040 GAGACC
BstXI 524 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2695 CCACGAGAATGG
BstYI 1133 AGATCT [GA]^GATC[CT]
2838 GGATCT
Eam1105I 194 GACGTGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
910 GACCAACTGTC
EcoNI 1243 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3474 CCTGGGACAGG
FspI 725 TGCGCA TGC^GCA
2643 TGCGCA
KpnI 1530 GGTACC GGTAC^C
3226 GGTACC
MflI 1133 AGATCT [GA]^GATC[CT]
2838 GGATCT
PaeR7I 1244 CTCGAG C^TCGAG
1524 CTCGAG
PflFI 1643 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACCCTGTC
PflMI 755 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAACATCTGG
PmaCI 2069 CACGTG CAC^GTG
3286 CACGTG
PmlI 2069 CACGTG CAC^GTG
3286 CACGTG
SacI 1238 GAGCTC GAGCT^C
2416 GAGCTC
SapI 3985 GAAGAGC GCTCTTC(1/4)
1315 GCTCTTC
SbfI 2941 CCTGCAGG CCTGCA^GG
3128 CCTGCAGG
SexAI 1788 ACCTGGT A^CC[AT]GGT
2592 ACCTGGT
SfiI 2506 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4124 GGCCCAAGTGGCC
SphI 2536 GCATGC GCATG^C
2640 GCATGC
Sse8387I 2941 CCTGCAGG CCTGCA^GG
3128 CCTGCAGG
Tth111I 1643 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2161 GACCCTGTC
Van91I 755 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1293 CCAACATCTGG
XhoI 1244 CTCGAG C^TCGAG
1524 CTCGAG
XhoII 1133 AGATCT [GA]^GATC[CT]
2838 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BssSI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI