##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I FseI
Hin1I HindIII KasI KpnI KspI
MaeII MluI MroI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AvaI 3840 CTCGGG C^[CT]CG[GA]G
AviII 803 TGCGCA TGC^GCA
BalI 2937 TGGCCA TGG^CCA
BanI 2133 GGTGCC G^G[CT][GA]CC
BglII 497 AGATCT A^GATCT
BpmI 3365 CTCCAG CTGGAG(16/14)
BsaBI 1243 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 414 GGTCTC GGTCTC(1/5)
BsoBI 3840 CTCGGG C^[CT]CG[GA]G
Bsp1407I 678 TGTACA T^GTACA
BspMI 485 GCAGGT ACCTGC(4/8)
BsrGI 678 TGTACA T^GTACA
BstAPI 1248 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 2937 TGGCCA [CT]^GGCC[GA]
EcoNI 3364 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1603 GATATC GAT^ATC
FspI 803 TGCGCA TGC^GCA
HaeII 673 GGCGCT [GA]GCGC^[CT]
HgaI 1995 GCGTC GACGC(5/10)
HpaI 3893 GTTAAC GTT^AAC
MamI 1243 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2937 TGGCCA TGG^CCA
MscI 2937 TGGCCA TGG^CCA
MspA1I 61 CAGCGG C[AC]G^C[GT]G
NcoI 420 CCATGG C^CATGG
PleI 2046 GAGTC GAGTC(4/5)
SapI 1496 GAAGAGC GCTCTTC(1/4)
ScaI 4057 AGTACT AGT^ACT
SphI 269 GCATGC GCATG^C
SspBI 678 TGTACA T^GTACA
SwaI 2709 ATTTAAAT ATTT^AAAT
XcmI 276 CCAGATGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1328 GTAGAC GT^[AC][GT]AC
1947 GTCTAC
AccII 1994 CGCG CG^CG
2178 CGCG
AflII 501 CTTAAG C^TTAAG
1745 CTTAAG
AflIII 3620 ACATGT A^C[GA][CT]GT
3992 ACATGT
AlwI 1242 GGATC GGATC(4/5)
3704 GGATC
AseI 1038 ATTAAT AT^TAAT
3233 ATTAAT
AsnI 1038 ATTAAT AT^TAAT
3233 ATTAAT
AspHI 2568 GAGCTC G[AT]GC[AT]^C
3514 GAGCTC
BclI 794 TGATCA T^GATCA
829 TGATCA
BfrI 501 CTTAAG C^TTAAG
1745 CTTAAG
BglI 1047 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1059 GCCGCCCAGGC
BsiHKAI 2568 GAGCTC G[AT]GC[AT]^C
3514 GAGCTC
BspLU11I 3620 ACATGT A^CATGT
3992 ACATGT
BsrDI 2273 GCAATG GCAATG(2/0)
2481 GCAATG
BstEII 104 GGTAACC G^GT[ACGT]ACC
2992 GGTGACC
BstPI 104 GGTAACC G^GT[ACGT]ACC
2992 GGTGACC
BstUI 1994 CGCG CG^CG
2178 CGCG
BstYI 497 AGATCT [GA]^GATC[CT]
3704 GGATCT
DsaI 172 CCACGG C^C[GA][CT]GG
420 CCATGG
EarI 2454 CTCTTC CTCTTC(1/4)
1496 GAAGAG
EcoO65I 104 GGTAACC G^GT[ACGT]ACC
2992 GGTGACC
EcoT14I 420 CCATGG C^C[AT][AT]GG
1151 CCAAGG
FauI 71 GCGGG CCCGC(4/6)
707 GCGGG
FbaI 794 TGATCA T^GATCA
829 TGATCA
HapII 248 CCGG C^CGG
3025 CCGG
HpaII 248 CCGG C^CGG
3025 CCGG
Ksp632I 2454 CTCTTC CTCTTC(1/4)
1496 GAAGAG
MflI 497 AGATCT [GA]^GATC[CT]
3704 GGATCT
MspI 248 CCGG C^CGG
3025 CCGG
MvnI 1994 CGCG CG^CG
2178 CGCG
PpuMI 439 AGGACCT [GA]G^G[AT]CC[CT]
3844 GGGACCT
PshBI 1038 ATTAAT AT^TAAT
3233 ATTAAT
PstI 1864 CTGCAG CTGCA^G
2636 CTGCAG
SacI 2568 GAGCTC GAGCT^C
3514 GAGCTC
StuI 1180 AGGCCT AGG^CCT
3297 AGGCCT
StyI 420 CCATGG C^C[AT][AT]GG
1151 CCAAGG
XhoII 497 AGATCT [GA]^GATC[CT]
3704 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AvaII BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EcoO109I EcoRI
EcoRII EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MfeI MnlI MseI MslI
MunI MvaI MwoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XmnI