##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       BcgI        BlpI        Bpu1102I    BseAI       
BsiWI       BsmI        Bsp1407I    BspEI       BsrGI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        Eam1105I    
Eco47III    Eco81I      EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MluI        MroI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SfiI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        3457  GACGTC               GACGT^C
AccI         1025  GTCTAC               GT^[AC][GT]AC
AflII        1577  CTTAAG               C^TTAAG
AspHI         238  GAGCAC               G[AT]GC[AT]^C
AvrII        1906  CCTAGG               C^CTAGG
BalI         1260  TGGCCA               TGG^CCA
BamHI        1788  GGATCC               G^GATCC
BciVI        3190  GTATCC               GTATCC(6/5)
BfrI         1577  CTTAAG               C^TTAAG
BglII        3105  AGATCT               A^GATCT
BlnI         1906  CCTAGG               C^CTAGG
BsaBI         435  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1401  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       238  GAGCAC               G[AT]GC[AT]^C
BsmBI         962  GAGACG               CGTCTC(1/5)
BspDI        1988  ATCGAT               AT^CGAT
BspHI        2742  TCATGA               T^CATGA
BspLU11I     1419  ACATGT               A^CATGT
BsrFI          98  GCCGGC               [GA]^CCGG[CT]
BssSI         447  CTCGTG               CACGAG(-5/-1)
BstAPI       1592  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         765  TTCGAA               TT^CGAA
Cfr10I         98  GCCGGC               [GA]^CCGG[CT]
ClaI         1988  ATCGAT               AT^CGAT
DraIII       3335  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1345  GACCTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1837  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI          124  CCGCGG               CCGC^GG
MamI          435  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1260  TGGCCA               TGG^CCA
MscI         1260  TGGCCA               TGG^CCA
NaeI           98  GCCGGC               GCC^GGC
NcoI          249  CCATGG               C^CATGG
NgoMIV         98  GCCGGC               G^CCGGC
NspV          765  TTCGAA               TT^CGAA
PflMI        2830  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         2742  TCATGA               T^CATGA
SacII         124  CCGCGG               CCGC^GG
SexAI        3206  ACCTGGT              A^CC[AT]GGT
SfuI          765  TTCGAA               TT^CGAA
SmaI           54  CCCGGG               CCC^GGG
StuI         2232  AGGCCT               AGG^CCT
Van91I       2830  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          54  CCCGGG               C^CCGGG
XmaI           54  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         125  CGCG                 CG^CG
              468  CGCG                
AflIII         31  ACACGT               A^C[GA][CT]GT
             1419  ACATGT              
AlwNI        1832  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2831  CAGGCCCTG           
Asp700I      1937  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2875  GAACGGCTTC          
BanII        1748  GAGCCC               G[GA]GC[CT]^C
             2009  GGGCTC              
BbeI          225  GGCGCC               GGCGC^C
             1775  GGCGCC              
BbrPI          32  CACGTG               CAC^GTG
             1011  CACGTG              
BbvCI         746  CCTCAGC              CCTCAGC(-5/-2)
             2333  CCTCAGC             
BclI          376  TGATCA               T^GATCA
             2796  TGATCA              
BglI           53  GCCCGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3285  GCCCTGGCGGC         
BsaAI          32  CACGTG               [CT]AC^GT[GA]
             1011  CACGTG              
BspMI        2999  ACCTGC               ACCTGC(4/8)
             1523  GCAGGT              
BsrBI         164  CCGCTC               CCGCTC(-3/-3)
             2789  GAGCGG              
BstUI         125  CGCG                 CG^CG
              468  CGCG                
EcoRI         843  GAATTC               G^AATTC
             3073  GAATTC              
FbaI          376  TGATCA               T^GATCA
             2796  TGATCA              
HaeII         225  GGCGCC               [GA]GCGC^[CT]
             1775  GGCGCC              
HgaI         1856  GACGC                GACGC(5/10)
             3282  GACGC               
KasI          225  GGCGCC               G^GCGCC
             1775  GGCGCC              
MfeI          991  CAATTG               C^AATTG
             2520  CAATTG              
MunI          991  CAATTG               C^AATTG
             2520  CAATTG              
MvnI          125  CGCG                 CG^CG
              468  CGCG                
NarI          225  GGCGCC               GG^CGCC
             1775  GGCGCC              
PmaCI          32  CACGTG               CAC^GTG
             1011  CACGTG              
PmlI           32  CACGTG               CAC^GTG
             1011  CACGTG              
PpuMI         218  AGGACCC              [GA]G^G[AT]CC[CT]
             2324  AGGACCT             
PstI          285  CTGCAG               CTGCA^G
             1104  CTGCAG              
SfoI          225  GGCGCC               GGC^GCC
             1775  GGCGCC              
XmnI         1937  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2875  GAACGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspI        AvaI        AvaII       
BanI        BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflFI       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XhoII