##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BcgI BlpI Bpu1102I BseAI
BsiWI BsmI Bsp1407I BspEI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MluI MroI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3457 GACGTC GACGT^C
AccI 1025 GTCTAC GT^[AC][GT]AC
AflII 1577 CTTAAG C^TTAAG
AspHI 238 GAGCAC G[AT]GC[AT]^C
AvrII 1906 CCTAGG C^CTAGG
BalI 1260 TGGCCA TGG^CCA
BamHI 1788 GGATCC G^GATCC
BciVI 3190 GTATCC GTATCC(6/5)
BfrI 1577 CTTAAG C^TTAAG
BglII 3105 AGATCT A^GATCT
BlnI 1906 CCTAGG C^CTAGG
BsaBI 435 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1401 ACCGGA [AT]^CCGG[AT]
BsiHKAI 238 GAGCAC G[AT]GC[AT]^C
BsmBI 962 GAGACG CGTCTC(1/5)
BspDI 1988 ATCGAT AT^CGAT
BspHI 2742 TCATGA T^CATGA
BspLU11I 1419 ACATGT A^CATGT
BsrFI 98 GCCGGC [GA]^CCGG[CT]
BssSI 447 CTCGTG CACGAG(-5/-1)
BstAPI 1592 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 765 TTCGAA TT^CGAA
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
ClaI 1988 ATCGAT AT^CGAT
DraIII 3335 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1345 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1837 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KspI 124 CCGCGG CCGC^GG
MamI 435 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1260 TGGCCA TGG^CCA
MscI 1260 TGGCCA TGG^CCA
NaeI 98 GCCGGC GCC^GGC
NcoI 249 CCATGG C^CATGG
NgoMIV 98 GCCGGC G^CCGGC
NspV 765 TTCGAA TT^CGAA
PflMI 2830 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2742 TCATGA T^CATGA
SacII 124 CCGCGG CCGC^GG
SexAI 3206 ACCTGGT A^CC[AT]GGT
SfuI 765 TTCGAA TT^CGAA
SmaI 54 CCCGGG CCC^GGG
StuI 2232 AGGCCT AGG^CCT
Van91I 2830 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 54 CCCGGG C^CCGGG
XmaI 54 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 125 CGCG CG^CG
468 CGCG
AflIII 31 ACACGT A^C[GA][CT]GT
1419 ACATGT
AlwNI 1832 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2831 CAGGCCCTG
Asp700I 1937 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2875 GAACGGCTTC
BanII 1748 GAGCCC G[GA]GC[CT]^C
2009 GGGCTC
BbeI 225 GGCGCC GGCGC^C
1775 GGCGCC
BbrPI 32 CACGTG CAC^GTG
1011 CACGTG
BbvCI 746 CCTCAGC CCTCAGC(-5/-2)
2333 CCTCAGC
BclI 376 TGATCA T^GATCA
2796 TGATCA
BglI 53 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3285 GCCCTGGCGGC
BsaAI 32 CACGTG [CT]AC^GT[GA]
1011 CACGTG
BspMI 2999 ACCTGC ACCTGC(4/8)
1523 GCAGGT
BsrBI 164 CCGCTC CCGCTC(-3/-3)
2789 GAGCGG
BstUI 125 CGCG CG^CG
468 CGCG
EcoRI 843 GAATTC G^AATTC
3073 GAATTC
FbaI 376 TGATCA T^GATCA
2796 TGATCA
HaeII 225 GGCGCC [GA]GCGC^[CT]
1775 GGCGCC
HgaI 1856 GACGC GACGC(5/10)
3282 GACGC
KasI 225 GGCGCC G^GCGCC
1775 GGCGCC
MfeI 991 CAATTG C^AATTG
2520 CAATTG
MunI 991 CAATTG C^AATTG
2520 CAATTG
MvnI 125 CGCG CG^CG
468 CGCG
NarI 225 GGCGCC GG^CGCC
1775 GGCGCC
PmaCI 32 CACGTG CAC^GTG
1011 CACGTG
PmlI 32 CACGTG CAC^GTG
1011 CACGTG
PpuMI 218 AGGACCC [GA]G^G[AT]CC[CT]
2324 AGGACCT
PstI 285 CTGCAG CTGCA^G
1104 CTGCAG
SfoI 225 GGCGCC GGC^GCC
1775 GGCGCC
XmnI 1937 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2875 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspI AvaI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII