##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbrPI BcgI BfrI
BglII BlnI BsaAI BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FseI FspI HincII HindII
HpaI KpnI KspI MamI MluI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2161 GACGTC GACGT^C
AccI 554 GTATAC GT^[AC][GT]AC
AccIII 795 TCCGGA T^CCGGA
Alw44I 646 GTGCAC G^TGCAC
AocI 379 CCTCAGG CC^T[ACGT]AGG
ApaI 12 GGGCCC GGGCC^C
ApaLI 646 GTGCAC G^TGCAC
AseI 1096 ATTAAT AT^TAAT
AsnI 1096 ATTAAT AT^TAAT
Asp700I 220 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2468 TGGCCA TGG^CCA
BamHI 186 GGATCC G^GATCC
BbeI 821 GGCGCC GGCGC^C
BciVI 2660 GGATAC GTATCC(6/5)
BclI 1904 TGATCA T^GATCA
BglI 2149 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3265 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3265 GCTGAGC GC^T[ACGT]AGC
BsaWI 795 TCCGGA [AT]^CCGG[AT]
BseAI 795 TCCGGA T^CCGGA
BseRI 1313 GAGGAG GAGGAG(10/8)
BsiEI 3392 CGGTCG CG[GA][CT]^CG
BsmBI 1703 GAGACG CGTCTC(1/5)
BspEI 795 TCCGGA T^CCGGA
BsrBI 3268 GAGCGG CCGCTC(-3/-3)
BsrDI 3344 CATTGC GCAATG(2/0)
Bst1107I 554 GTATAC GTA^TAC
BstZ17I 554 GTATAC GTA^TAC
Bsu36I 379 CCTCAGG CC^T[ACGT]AGG
CelII 3265 GCTGAGC GC^T[ACGT]AGC
DraIII 3059 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 526 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 379 CCTCAGG CC^T[ACGT]AGG
FbaI 1904 TGATCA T^GATCA
HindIII 3301 AAGCTT A^AGCTT
KasI 821 GGCGCC G^GCGCC
MfeI 2786 CAATTG C^AATTG
MluNI 2468 TGGCCA TGG^CCA
MroI 795 TCCGGA T^CCGGA
MscI 2468 TGGCCA TGG^CCA
MunI 2786 CAATTG C^AATTG
NarI 821 GGCGCC GG^CGCC
NdeI 2510 CATATG CA^TATG
PaeR7I 3172 CTCGAG C^TCGAG
PshBI 1096 ATTAAT AT^TAAT
SacI 2884 GAGCTC GAGCT^C
SapI 2341 GCTCTTC GCTCTTC(1/4)
ScaI 2275 AGTACT AGT^ACT
SexAI 342 ACCTGGT A^CC[AT]GGT
SfoI 821 GGCGCC GGC^GCC
SmaI 19 CCCGGG CCC^GGG
SpeI 1156 ACTAGT A^CTAGT
StuI 163 AGGCCT AGG^CCT
XhoI 3172 CTCGAG C^TCGAG
XmaCI 19 CCCGGG C^CCGGG
XmaI 19 CCCGGG C^CCGGG
XmnI 220 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspI 1765 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2899 GACAGTGTC
BbsI 2820 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BbvCI 759 CCTCAGC CCTCAGC(-5/-2)
1938 GCTGAGG
BpuAI 2820 GAAGAC GAAGAC(2/6)
1069 GTCTTC
BsaI 3175 GAGACC GGTCTC(1/5)
2724 GGTCTC
BspMI 455 ACCTGC ACCTGC(4/8)
2481 GCAGGT
DraI 3465 TTTAAA TTT^AAA
3494 TTTAAA
EcoNI 2861 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3022 CCTCTAGAAGG
EcoT22I 872 ATGCAT ATGCA^T
3274 ATGCAT
HaeII 821 GGCGCC [GA]GCGC^[CT]
1730 AGCGCC
HgaI 844 GACGC GACGC(5/10)
1884 GACGC
NaeI 56 GCCGGC GCC^GGC
3142 GCCGGC
NcoI 330 CCATGG C^CATGG
1436 CCATGG
NgoMIV 56 GCCGGC G^CCGGC
3142 GCCGGC
NsiI 872 ATGCAT ATGCA^T
3274 ATGCAT
PflFI 1765 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2899 GACAGTGTC
PpuMI 1457 AGGACCC [GA]G^G[AT]CC[CT]
3018 AGGACCT
PstI 736 CTGCAG CTGCA^G
2584 CTGCAG
SfcI 736 CTGCAG C^T[GA][CT]AG
2584 CTGCAG
SmlI 2038 CTCAAG C^T[CT][GA]AG
3172 CTCGAG
SphI 459 GCATGC GCATG^C
1668 GCATGC
Tth111I 1765 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2899 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII