##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspI AviII AvrII BalI
BanI BbeI BbrPI BbvCI BciVI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsiWI BsmBI BsmI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eco47III Eco81I EcoNI EcoO65I EcoT14I
EcoT22I FspI HaeII KasI KpnI
MluI MluNI MroI MscI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI StyI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 1976 GACGCC G[GA]^CG[CT]C
AflII 3104 CTTAAG C^TTAAG
AflIII 2103 ACATGT A^C[GA][CT]GT
Alw44I 3557 GTGCAC G^TGCAC
ApaLI 3557 GTGCAC G^TGCAC
AspHI 3557 GTGCAC G[AT]GC[AT]^C
AvaI 1121 CTCGGG C^[CT]CG[GA]G
BclI 1191 TGATCA T^GATCA
BfrI 3104 CTTAAG C^TTAAG
BglII 137 AGATCT A^GATCT
BsaHI 1976 GACGCC G[GA]^CG[CT]C
BsaWI 1836 ACCGGA [AT]^CCGG[AT]
BsgI 1217 GTGCAG GTGCAG(16/14)
BsiHKAI 3557 GTGCAC G[AT]GC[AT]^C
BsoBI 1121 CTCGGG C^[CT]CG[GA]G
BspLU11I 2103 ACATGT A^CATGT
BspMI 471 GCAGGT ACCTGC(4/8)
BsrDI 3784 CATTGC GCAATG(2/0)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BstAPI 510 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1514 TTCGAA TT^CGAA
BstXI 1355 CCATTGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 444 GCGC GCG^C
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
EcoRV 3191 GATATC GAT^ATC
FbaI 1191 TGATCA T^GATCA
FseI 5 GGCCGGCC GGCCGG^CC
HhaI 444 GCGC GCG^C
Hin1I 1976 GACGCC G[GA]^CG[CT]C
HinP1I 444 GCGC G^CGC
HindIII 1894 AAGCTT A^AGCTT
HpaI 3548 GTTAAC GTT^AAC
MaeII 2604 ACGT A^CGT
NaeI 6 GCCGGC GCC^GGC
NgoMIV 6 GCCGGC G^CCGGC
NspV 1514 TTCGAA TT^CGAA
PpuMI 2366 AGGACCT [GA]G^G[AT]CC[CT]
SalI 51 GTCGAC G^TCGAC
SfcI 1212 CTACAG C^T[GA][CT]AG
SfuI 1514 TTCGAA TT^CGAA
SphI 3089 GCATGC GCATG^C
XbaI 3908 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 51 GTCGAC GT^[AC][GT]AC
1199 GTAGAC
AhdI 2345 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3405 GACTTAATGTC
Asp700I 2230 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2569 GAATTAATTC
AspEI 2345 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3405 GACTTAATGTC
BamHI 1884 GGATCC G^GATCC
2032 GGATCC
BcgI 500 CGAAAATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1989 GCAAGCTGTTCG
BsaBI 616 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1450 GATACAGATC
BsiEI 8 CGGCCG CG[GA][CT]^CG
14 CGGCCG
BsmFI 1084 GGGAC GGGAC(10/14)
2495 GTCCC
Bsp1407I 3684 TGTACA T^GTACA
3922 TGTACA
BspHI 2193 TCATGA T^CATGA
2977 TCATGA
BsrGI 3684 TGTACA T^GTACA
3922 TGTACA
DraII 2366 AGGACCT [GA]G^G[ACGT]CC[CT]
2770 AGGCCCT
DsaI 11 CCGCGG C^C[GA][CT]GG
63 CCGCGG
EaeI 8 CGGCCG [CT]^GGCC[GA]
14 CGGCCG
EagI 8 CGGCCG C^GGCCG
14 CGGCCG
Eam1105I 2345 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3405 GACTTAATGTC
EclXI 8 CGGCCG C^GGCCG
14 CGGCCG
Eco52I 8 CGGCCG C^GGCCG
14 CGGCCG
EcoO109I 2366 AGGACCT [GA]G^G[ACGT]CC[CT]
2770 AGGCCCT
EcoRI 2823 GAATTC G^AATTC
3716 GAATTC
FauI 56 CCCGC CCCGC(4/6)
65 GCGGG
HgaI 1976 GACGC GACGC(5/10)
46 GCGTC
KspI 11 CCGCGG CCGC^GG
63 CCGCGG
MamI 616 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1450 GATACAGATC
MfeI 1652 CAATTG C^AATTG
2483 CAATTG
MspA1I 11 CCGCGG C[AC]G^C[GT]G
63 CCGCGG
MunI 1652 CAATTG C^AATTG
2483 CAATTG
PflMI 181 CCATAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
997 CCACTTCCTGG
RcaI 2193 TCATGA T^CATGA
2977 TCATGA
SacII 11 CCGCGG CCGC^GG
63 CCGCGG
SapI 33 GCTCTTC GCTCTTC(1/4)
988 GCTCTTC
SspBI 3684 TGTACA T^GTACA
3922 TGTACA
Tsp45I 439 GTCAC ^GT[GC]AC
2702 GTGAC
Van91I 181 CCATAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
997 CCACTTCCTGG
XmnI 2230 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2569 GAATTAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII