##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AcyI AgeI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbeI
BclI BglI BlnI BlpI Bpu1102I
BsaHI BsiEI BsiWI BsmBI BsmI
BspDI BsrFI BssHII Bst1107I BstAPI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
EcoT22I FauI FbaI FseI FspI
Hin1I KasI KpnI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI PvuI RsrII
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 3821 TCCGGA T^CCGGA
AclI 2878 AACGTT AA^CGTT
AflII 2516 CTTAAG C^TTAAG
AlwNI 2948 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1199 CCTAAGG CC^T[ACGT]AGG
BalI 1603 TGGCCA TGG^CCA
BbrPI 3254 CACGTG CAC^GTG
BcgI 1876 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2904 GGATAC GTATCC(6/5)
BfrI 2516 CTTAAG C^TTAAG
BsaI 3492 GGTCTC GGTCTC(1/5)
BsaWI 3821 TCCGGA [AT]^CCGG[AT]
BseAI 3821 TCCGGA T^CCGGA
BspEI 3821 TCCGGA T^CCGGA
BspHI 1921 TCATGA T^CATGA
BspMI 3930 GCAGGT ACCTGC(4/8)
BsrBI 1544 GAGCGG CCGCTC(-3/-3)
BssSI 3516 CACGAG CACGAG(-5/-1)
BstBI 2784 TTCGAA TT^CGAA
Bsu36I 1199 CCTAAGG CC^T[ACGT]AGG
EaeI 1603 TGGCCA [CT]^GGCC[GA]
Eco81I 1199 CCTAAGG CC^T[ACGT]AGG
EcoRV 920 GATATC GAT^ATC
HindIII 3388 AAGCTT A^AGCTT
HpaI 709 GTTAAC GTT^AAC
MluNI 1603 TGGCCA TGG^CCA
MroI 3821 TCCGGA T^CCGGA
MscI 1603 TGGCCA TGG^CCA
NheI 3564 GCTAGC G^CTAGC
NspV 2784 TTCGAA TT^CGAA
PflMI 255 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3254 CACGTG CAC^GTG
PmlI 3254 CACGTG CAC^GTG
Psp1406I 2878 AACGTT AA^CGTT
PvuII 3730 CAGCTG CAG^CTG
RcaI 1921 TCATGA T^CATGA
SacI 1823 GAGCTC GAGCT^C
SalI 4027 GTCGAC G^TCGAC
SapI 3678 GCTCTTC GCTCTTC(1/4)
SexAI 2808 ACCAGGT A^CC[AT]GGT
SfuI 2784 TTCGAA TT^CGAA
SpeI 4009 ACTAGT A^CTAGT
SphI 4001 GCATGC GCATG^C
SspI 1852 AATATT AAT^ATT
Van91I 255 CCACGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Alw44I 3251 GTGCAC G^TGCAC
3548 GTGCAC
Aor51HI 102 AGCGCT AGC^GCT
3675 AGCGCT
ApaLI 3251 GTGCAC G^TGCAC
3548 GTGCAC
AvaI 1874 CCCGAG C^[CT]CG[GA]G
3713 CTCGGG
BanI 812 GGCACC G^G[CT][GA]CC
827 GGCACC
BanII 1823 GAGCTC G[GA]GC[CT]^C
3107 GGGCTC
BbvCI 1484 CCTCAGC CCTCAGC(-5/-2)
2899 GCTGAGG
BcnI 2629 CCGGG CC^[GC]GG
3074 CCCGG
BglII 2239 AGATCT A^GATCT
2461 AGATCT
BsaAI 2013 TACGTG [CT]AC^GT[GA]
3254 CACGTG
BsaBI 1913 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2521 GATGAAAATC
BsgI 1402 CTGCAC GTGCAG(16/14)
1574 GTGCAG
BsoBI 1874 CCCGAG C^[CT]CG[GA]G
3713 CTCGGG
Bsp1407I 457 TGTACA T^GTACA
600 TGTACA
BspLU11I 1416 ACATGT A^CATGT
1620 ACATGT
BsrGI 457 TGTACA T^GTACA
600 TGTACA
BstEII 1465 GGTCACC G^GT[ACGT]ACC
3418 GGTAACC
BstPI 1465 GGTCACC G^GT[ACGT]ACC
3418 GGTAACC
DraI 2555 TTTAAA TTT^AAA
2987 TTTAAA
Eco47III 102 AGCGCT AGC^GCT
3675 AGCGCT
EcoO65I 1465 GGTCACC G^GT[ACGT]ACC
3418 GGTAACC
EcoRI 1151 GAATTC G^AATTC
2109 GAATTC
HgaI 1013 GACGC GACGC(5/10)
639 GCGTC
HincII 709 GTTAAC GT[CT]^[GA]AC
4027 GTCGAC
HindII 709 GTTAAC GT[CT]^[GA]AC
4027 GTCGAC
KspI 36 CCGCGG CCGC^GG
847 CCGCGG
MamI 1913 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2521 GATGAAAATC
MfeI 1353 CAATTG C^AATTG
2997 CAATTG
MunI 1353 CAATTG C^AATTG
2997 CAATTG
NciI 2629 CCGGG CC^[GC]GG
3074 CCCGG
PleI 122 GAGTC GAGTC(4/5)
3139 GAGTC
SacII 36 CCGCGG CCGC^GG
847 CCGCGG
SspBI 457 TGTACA T^GTACA
600 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI ApoI
AspEI AspHI AvaII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV NspI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII