##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AlwNI AocI ApaI AscI
AspI AviII BbrPI BcgI BciVI
BfrI BglI BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BspEI BsrFI BssHII BstBI BstEII
BstPI BstUI Bsu36I Cfr10I CpoI
DrdI DsaI EagI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KspI MamI MluI MroI MvnI
NaeI NcoI NdeI NgoMIV NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 161 GGTACC G^GTACC
AccI 2250 GTATAC GT^[AC][GT]AC
AcyI 3823 GGCGCC G[GA]^CG[CT]C
AhdI 2030 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2153 GTGCAC G^TGCAC
ApaLI 2153 GTGCAC G^TGCAC
AseI 3270 ATTAAT AT^TAAT
AsnI 3270 ATTAAT AT^TAAT
Asp718I 161 GGTACC G^GTACC
AspEI 2030 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2974 CCTAGG C^CTAGG
BalI 236 TGGCCA TGG^CCA
BamHI 1823 GGATCC G^GATCC
BbeI 3823 GGCGCC GGCGC^C
BbvCI 1183 CCTCAGC CCTCAGC(-5/-2)
BlnI 2974 CCTAGG C^CTAGG
BlpI 136 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 136 GCTTAGC GC^T[ACGT]AGC
BsaHI 3823 GGCGCC G[GA]^CG[CT]C
BspDI 3036 ATCGAT AT^CGAT
BspMI 3747 ACCTGC ACCTGC(4/8)
BsrBI 1505 GAGCGG CCGCTC(-3/-3)
Bst1107I 2250 GTATAC GTA^TAC
BstAPI 3503 GCAGTAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2250 GTATAC GTA^TAC
CelII 136 GCTTAGC GC^T[ACGT]AGC
ClaI 3036 ATCGAT AT^CGAT
DraII 2946 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 240 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 236 TGGCCA [CT]^GGCC[GA]
Eam1105I 2030 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2946 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 718 CCCGC CCCGC(4/6)
HgaI 894 GCGTC GACGC(5/10)
Hin1I 3823 GGCGCC G[GA]^CG[CT]C
KasI 3823 GGCGCC G^GCGCC
KpnI 161 GGTACC GGTAC^C
MfeI 565 CAATTG C^AATTG
MluNI 236 TGGCCA TGG^CCA
MscI 236 TGGCCA TGG^CCA
MspA1I 1679 CAGCTG C[AC]G^C[GT]G
MunI 565 CAATTG C^AATTG
NarI 3823 GGCGCC GG^CGCC
PshAI 816 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3270 ATTAAT AT^TAAT
PvuII 1679 CAGCTG CAG^CTG
ScaI 2294 AGTACT AGT^ACT
SfoI 3823 GGCGCC GGC^GCC
SpeI 3430 ACTAGT A^CTAGT
SspI 3389 AATATT AAT^ATT
XcmI 1224 CCAAAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1147 ACATGT A^C[GA][CT]GT
2254 ACATGT
Aor51HI 744 AGCGCT AGC^GCT
765 AGCGCT
BanII 1097 GAGCTC G[GA]GC[CT]^C
3371 GAGCTC
BclI 1638 TGATCA T^GATCA
3627 TGATCA
BcnI 1926 CCGGG CC^[GC]GG
1977 CCGGG
BglII 15 AGATCT A^GATCT
1312 AGATCT
BsaI 878 GAGACC GGTCTC(1/5)
3022 GAGACC
BsgI 3326 GTGCAG GTGCAG(16/14)
3338 GTGCAG
BsmFI 1155 GTCCC GGGAC(10/14)
2288 GTCCC
Bsp1407I 2185 TGTACA T^GTACA
3532 TGTACA
BspHI 1006 TCATGA T^CATGA
2628 TCATGA
BspLU11I 1147 ACATGT A^CATGT
2254 ACATGT
BsrDI 49 GCAATG GCAATG(2/0)
498 GCAATG
BsrGI 2185 TGTACA T^GTACA
3532 TGTACA
BssSI 2056 CACGAG CACGAG(-5/-1)
1163 CTCGTG
Eco47III 744 AGCGCT AGC^GCT
765 AGCGCT
EcoT22I 2716 ATGCAT ATGCA^T
3510 ATGCAT
FbaI 1638 TGATCA T^GATCA
3627 TGATCA
HapII 1926 CCGG C^CGG
1977 CCGG
HindIII 304 AAGCTT A^AGCTT
2665 AAGCTT
HpaII 1926 CCGG C^CGG
1977 CCGG
MslI 370 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1159 CATCCTCGTG
MspI 1926 CCGG C^CGG
1977 CCGG
NciI 1926 CCGGG CC^[GC]GG
1977 CCGGG
NsiI 2716 ATGCAT ATGCA^T
3510 ATGCAT
RcaI 1006 TCATGA T^CATGA
2628 TCATGA
SacI 1097 GAGCTC GAGCT^C
3371 GAGCTC
SspBI 2185 TGTACA T^GTACA
3532 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NheI NlaIII NlaIV NspI
PaeR7I PleI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII XmnI