##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
Acc65I AclI AflII Alw44I ApaLI
AscI Asp718I AspI AviII BbeI
BciVI BfrI BsaBI BsiWI BspHI
BsrBI Bst1107I BstZ17I CpoI EcoT22I
FseI FspI KasI KpnI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PmeI Psp1406I PvuI RcaI RsrII
SalI SbfI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2310 GACGTC GACGT^C
AcyI 2310 GACGTC G[GA]^CG[CT]C
AgeI 6031 ACCGGT A^CCGGT
Aor51HI 51 AGCGCT AGC^GCT
Asp700I 541 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 128 CCTAGG C^CTAGG
BbrPI 80 CACGTG CAC^GTG
BclI 2747 TGATCA T^GATCA
BglII 7441 AGATCT A^GATCT
BlnI 128 CCTAGG C^CTAGG
BlpI 654 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 654 GCTGAGC GC^T[ACGT]AGC
BsaHI 2310 GACGTC G[GA]^CG[CT]C
BssHII 11 GCGCGC G^CGCGC
BstBI 1003 TTCGAA TT^CGAA
CelII 654 GCTGAGC GC^T[ACGT]AGC
DraI 7491 TTTAAA TTT^AAA
DrdI 5767 GACAGTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1719 CGGCCG C^GGCCG
EclXI 1719 CGGCCG C^GGCCG
Eco47III 51 AGCGCT AGC^GCT
Eco52I 1719 CGGCCG C^GGCCG
EcoRV 2628 GATATC GAT^ATC
FbaI 2747 TGATCA T^GATCA
Hin1I 2310 GACGTC G[GA]^CG[CT]C
HindIII 543 AAGCTT A^AGCTT
HpaI 6787 GTTAAC GTT^AAC
KspI 3993 CCGCGG CCGC^GG
NdeI 6413 CATATG CA^TATG
NspV 1003 TTCGAA TT^CGAA
PinAI 6031 ACCGGT A^CCGGT
PmaCI 80 CACGTG CAC^GTG
PmlI 80 CACGTG CAC^GTG
SacII 3993 CCGCGG CCGC^GG
SfiI 5689 GGCCCATCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1003 TTCGAA TT^CGAA
SspI 7385 AATATT AAT^ATT
XbaI 2490 TCTAGA T^CTAGA
XmnI 541 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BsaAI 80 CACGTG [CT]AC^GT[GA]
2415 CACGTA
BsmI 4947 GAATGC GAATGC(1/-1)
2390 GCATTC
Bsp1407I 2252 TGTACA T^GTACA
7211 TGTACA
BspDI 2776 ATCGAT AT^CGAT
2833 ATCGAT
BsrGI 2252 TGTACA T^GTACA
7211 TGTACA
BssSI 2029 CACGAG CACGAG(-5/-1)
2266 CACGAG
BstAPI 1952 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6412 GCATATGCTGC
BstEII 2679 GGTGACC G^GT[ACGT]ACC
6034 GGTTACC
BstPI 2679 GGTGACC G^GT[ACGT]ACC
6034 GGTTACC
ClaI 2776 ATCGAT AT^CGAT
2833 ATCGAT
DraIII 2638 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
6906 CACTGGGTG
EcoO65I 2679 GGTGACC G^GT[ACGT]ACC
6034 GGTTACC
EcoRI 5008 GAATTC G^AATTC
6445 GAATTC
MfeI 1146 CAATTG C^AATTG
6206 CAATTG
MunI 1146 CAATTG C^AATTG
6206 CAATTG
PaeR7I 4507 CTCGAG C^TCGAG
5857 CTCGAG
PflMI 423 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3360 CCAGTGGGTGG
SapI 2385 GAAGAGC GCTCTTC(1/4)
5910 GAAGAGC
SspBI 2252 TGTACA T^GTACA
7211 TGTACA
Van91I 423 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3360 CCAGTGGGTGG
XhoI 4507 CTCGAG C^TCGAG
5857 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AhdI AluI AlwI
AlwNI AocI ApaI ApoI AseI
AsnI AspEI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI