##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AcyI AgeI
AocI Aor51HI AscI Asp718I AspI
BalI BbeI BbrPI BsaHI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
CpoI DraIII EagI EarI EclXI
Eco47III Eco52I Eco81I EcoNI FseI
HaeII HgaI Hin1I KasI KpnI
Ksp632I KspI MluI MluNI MscI
NaeI NarI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SphI Sse8387I SspBI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 1687 CGCG CG^CG
AccIII 1157 TCCGGA T^CCGGA
AhdI 5159 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 7669 GTGCAC G^TGCAC
AlwNI 2355 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1475 GGGCCC GGGCC^C
ApaLI 7669 GTGCAC G^TGCAC
Asp700I 5260 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5159 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2293 TGCGCA TGC^GCA
AvrII 6973 CCTAGG C^CTAGG
BamHI 975 GGATCC G^GATCC
BbvCI 74 CCTCAGC CCTCAGC(-5/-2)
BcgI 1924 GCAAATGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7456 TGATCA T^GATCA
BlnI 6973 CCTAGG C^CTAGG
BlpI 1335 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1335 GCTGAGC GC^T[ACGT]AGC
BsaAI 6374 TACGTA [CT]AC^GT[GA]
BseAI 1157 TCCGGA T^CCGGA
BspEI 1157 TCCGGA T^CCGGA
BsrBI 1302 CCGCTC CCGCTC(-3/-3)
BstEII 6543 GGTCACC G^GT[ACGT]ACC
BstPI 6543 GGTCACC G^GT[ACGT]ACC
BstUI 1687 CGCG CG^CG
CelII 1335 GCTGAGC GC^T[ACGT]AGC
DrdI 2373 GACGGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1587 CGGCCA [CT]^GGCC[GA]
Eam1105I 5159 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 6543 GGTCACC G^GT[ACGT]ACC
EcoRV 2457 GATATC GAT^ATC
EcoT22I 7042 ATGCAT ATGCA^T
FauI 1688 GCGGG CCCGC(4/6)
FbaI 7456 TGATCA T^GATCA
FspI 2293 TGCGCA TGC^GCA
MroI 1157 TCCGGA T^CCGGA
MvnI 1687 CGCG CG^CG
NsiI 7042 ATGCAT ATGCA^T
PacI 4786 TTAATTAA TTAAT^TAA
PflMI 5973 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2644 CTGCAG CTGCA^G
SacI 1849 GAGCTC GAGCT^C
SmaI 665 CCCGGG CCC^GGG
SnaBI 6374 TACGTA TAC^GTA
SpeI 5212 ACTAGT A^CTAGT
SwaI 3073 ATTTAAAT ATTT^AAAT
Van91I 5973 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4716 TCTAGA T^CTAGA
XmaCI 665 CCCGGG C^CCGGG
XmaI 665 CCCGGG C^CCGGG
XmnI 5260 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AclI 2193 AACGTT AA^CGTT
5295 AACGTT
BglI 1234 GCCCTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1271 GCCTCCTGGGC
BsaWI 1157 TCCGGA [AT]^CCGG[AT]
1944 ACCGGA
BspDI 2614 ATCGAT AT^CGAT
6739 ATCGAT
BspMI 2290 ACCTGC ACCTGC(4/8)
2041 GCAGGT
ClaI 2614 ATCGAT AT^CGAT
6739 ATCGAT
MfeI 988 CAATTG C^AATTG
4259 CAATTG
MunI 988 CAATTG C^AATTG
4259 CAATTG
NdeI 3962 CATATG CA^TATG
4574 CATATG
Psp1406I 2193 AACGTT AA^CGTT
5295 AACGTT
SfiI 1233 GGCCCTCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1270 GGCCTCCTGGGCC
StuI 205 AGGCCT AGG^CCT
906 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII