##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AseI AsnI AspI AvrII
BfrI BlnI BsiWI BssHII Bst1107I
BstBI BstZ17I CpoI DraI FseI
HindIII HpaI KspI MfeI MluI
MunI NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SfiI SfuI SgrAI SnaBI SpeI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 4384 GACGTC GACGT^C
Acc65I 2654 GGTACC G^GTACC
AccII 748 CGCG CG^CG
AhdI 6846 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 722 AGCGCT AGC^GCT
Asp700I 5362 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2654 GGTACC G^GTACC
AspEI 6846 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3569 TGCGCA TGC^GCA
BsiEI 958 CGGCCG CG[GA][CT]^CG
Bsp1407I 1286 TGTACA T^GTACA
BspDI 6157 ATCGAT AT^CGAT
BsrGI 1286 TGTACA T^GTACA
BstUI 748 CGCG CG^CG
ClaI 6157 ATCGAT AT^CGAT
DraIII 3645 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 958 CGGCCG C^GGCCG
Eam1105I 6846 GACTGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 958 CGGCCG C^GGCCG
Eco47III 722 AGCGCT AGC^GCT
Eco52I 958 CGGCCG C^GGCCG
EcoRV 553 GATATC GAT^ATC
FspI 3569 TGCGCA TGC^GCA
KpnI 2654 GGTACC GGTAC^C
MvnI 748 CGCG CG^CG
NcoI 5732 CCATGG C^CATGG
SalI 6893 GTCGAC G^TCGAC
SbfI 4550 CCTGCAGG CCTGCA^GG
ScaI 6788 AGTACT AGT^ACT
SmaI 7174 CCCGGG CCC^GGG
Sse8387I 4550 CCTGCAGG CCTGCA^GG
SspBI 1286 TGTACA T^GTACA
XbaI 4914 TCTAGA T^CTAGA
XmaCI 7174 CCCGGG C^CCGGG
XmaI 7174 CCCGGG C^CCGGG
XmnI 5362 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 506 TCCGGA T^CCGGA
4440 TCCGGA
AlwNI 1213 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
4156 CAGGCGCTG
AocI 5020 CCTAAGG CC^T[ACGT]AGG
6065 CCTCAGG
BamHI 1158 GGATCC G^GATCC
2805 GGATCC
BbeI 4059 GGCGCC GGCGC^C
6526 GGCGCC
BbrPI 1786 CACGTG CAC^GTG
3648 CACGTG
BseAI 506 TCCGGA T^CCGGA
4440 TCCGGA
BspEI 506 TCCGGA T^CCGGA
4440 TCCGGA
BspLU11I 1724 ACATGT A^CATGT
1754 ACATGT
BsrFI 4062 GCCGGC [GA]^CCGG[CT]
6016 GCCGGC
BssSI 7043 CACGAG CACGAG(-5/-1)
175 CTCGTG
BstEII 984 GGTGACC G^GT[ACGT]ACC
5262 GGTGACC
BstPI 984 GGTGACC G^GT[ACGT]ACC
5262 GGTGACC
Bsu36I 5020 CCTAAGG CC^T[ACGT]AGG
6065 CCTCAGG
Cfr10I 4062 GCCGGC [GA]^CCGG[CT]
6016 GCCGGC
DrdI 613 GACTCAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4036 GACTCCAATGTC
Eco81I 5020 CCTAAGG CC^T[ACGT]AGG
6065 CCTCAGG
EcoO65I 984 GGTGACC G^GT[ACGT]ACC
5262 GGTGACC
EcoT22I 1202 ATGCAT ATGCA^T
2248 ATGCAT
KasI 4059 GGCGCC G^GCGCC
6526 GGCGCC
MroI 506 TCCGGA T^CCGGA
4440 TCCGGA
NaeI 4062 GCCGGC GCC^GGC
6016 GCCGGC
NarI 4059 GGCGCC GG^CGCC
6526 GGCGCC
NdeI 3815 CATATG CA^TATG
6982 CATATG
NgoMIV 4062 GCCGGC G^CCGGC
6016 GCCGGC
NsiI 1202 ATGCAT ATGCA^T
2248 ATGCAT
PaeR7I 3375 CTCGAG C^TCGAG
6875 CTCGAG
PmaCI 1786 CACGTG CAC^GTG
3648 CACGTG
PmlI 1786 CACGTG CAC^GTG
3648 CACGTG
SfoI 4059 GGCGCC GGC^GCC
6526 GGCGCC
SspI 915 AATATT AAT^ATT
6106 AATATT
XhoI 3375 CTCGAG C^TCGAG
6875 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII