##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
Asp718I AviII BbrPI BcgI BsaBI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BsrBI BsrFI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI DrdI
EagI EclXI Eco52I EcoNI EcoRI
FseI FspI KpnI KspI MamI
MluI MroI NaeI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3403 GTCTAC GT^[AC][GT]AC
AflII 6476 CTTAAG C^TTAAG
AflIII 3787 ACATGT A^C[GA][CT]GT
AhdI 4788 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 7599 GTGCAC G^TGCAC
Aor51HI 267 AGCGCT AGC^GCT
ApaLI 7599 GTGCAC G^TGCAC
AspEI 4788 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5480 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 6755 CTCGGG C^[CT]CG[GA]G
BalI 2295 TGGCCA TGG^CCA
BbeI 152 GGCGCC GGCGC^C
BbvCI 899 CCTCAGC CCTCAGC(-5/-2)
BciVI 1771 GTATCC GTATCC(6/5)
BfrI 6476 CTTAAG C^TTAAG
BglI 4055 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2195 CACGTA [CT]AC^GT[GA]
BsoBI 6755 CTCGGG C^[CT]CG[GA]G
BspLU11I 3787 ACATGT A^CATGT
BssSI 3432 CACGAG CACGAG(-5/-1)
BstBI 3321 TTCGAA TT^CGAA
DsaI 4776 CCACGG C^C[GA][CT]GG
Eam1105I 4788 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 267 AGCGCT AGC^GCT
EcoRV 6609 GATATC GAT^ATC
HindIII 3520 AAGCTT A^AGCTT
KasI 152 GGCGCC G^GCGCC
MluNI 2295 TGGCCA TGG^CCA
MscI 2295 TGGCCA TGG^CCA
NarI 152 GGCGCC GG^CGCC
NspV 3321 TTCGAA TT^CGAA
PflFI 5480 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 6218 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1999 GCTCTTC GCTCTTC(1/4)
ScaI 2899 AGTACT AGT^ACT
SfoI 152 GGCGCC GGC^GCC
SfuI 3321 TTCGAA TT^CGAA
SphI 256 GCATGC GCATG^C
Tth111I 5480 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 7101 CCATGTTGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 249 GGGCCC GGGCC^C
5737 GGGCCC
AvrII 3274 CCTAGG C^CTAGG
5428 CCTAGG
BlnI 3274 CCTAGG C^CTAGG
5428 CCTAGG
BsaWI 967 TCCGGT [AT]^CCGG[AT]
5014 ACCGGA
BsmBI 2010 GAGACG CGTCTC(1/5)
5979 GAGACG
Bsp1407I 385 TGTACA T^GTACA
7528 TGTACA
BsrGI 385 TGTACA T^GTACA
7528 TGTACA
BstEII 2465 GGTGACC G^GT[ACGT]ACC
4838 GGTTACC
BstPI 2465 GGTGACC G^GT[ACGT]ACC
4838 GGTTACC
DraIII 4728 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5787 CACTTGGTG
EcoO65I 2465 GGTGACC G^GT[ACGT]ACC
4838 GGTTACC
HaeII 152 GGCGCC [GA]GCGC^[CT]
267 AGCGCT
HgaI 5320 GACGC GACGC(5/10)
134 GCGTC
HpaI 2492 GTTAAC GTT^AAC
5924 GTTAAC
NdeI 2912 CATATG CA^TATG
4268 CATATG
SexAI 402 ACCAGGT A^CC[AT]GGT
544 ACCTGGT
SspBI 385 TGTACA T^GTACA
7528 TGTACA
StuI 661 AGGCCT AGG^CCT
5425 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AseI AsnI Asp700I AspHI
AvaII BamHI BanI BanII BbsI
BbvI BclI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI