################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AccI AccIII AclI AcyI AgeI AhdI ApaI AscI AspEI AspI BbeI BclI BlpI Bpu1102I BsaHI BseAI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI Bst1107I BstZ17I CelII CpoI DrdI EagI Eam1105I EclXI Eco52I FbaI FseI Hin1I KasI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 3514 GGTACC G^GTACC AccII 4096 CGCG CG^CG AflII 1260 CTTAAG C^TTAAG Aor51HI 6806 AGCGCT AGC^GCT Asp718I 3514 GGTACC G^GTACC AviII 2336 TGCGCA TGC^GCA BanII 6376 GAGCTC G[GA]GC[CT]^C BbrPI 3509 CACGTG CAC^GTG BcgI 6069 CGAAAATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 7678 GGATAC GTATCC(6/5) BfrI 1260 CTTAAG C^TTAAG BglI 1378 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC Bsp1407I 823 TGTACA T^GTACA BspDI 509 ATCGAT AT^CGAT BspMI 2471 ACCTGC ACCTGC(4/8) BsrFI 5342 ACCGGC [GA]^CCGG[CT] BsrGI 823 TGTACA T^GTACA BstUI 4096 CGCG CG^CG Cfr10I 5342 ACCGGC [GA]^CCGG[CT] ClaI 509 ATCGAT AT^CGAT Eco47III 6806 AGCGCT AGC^GCT EcoNI 1211 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 2336 TGCGCA TGC^GCA HaeII 6806 AGCGCT [GA]GCGC^[CT] KpnI 3514 GGTACC GGTAC^C MvnI 4096 CGCG CG^CG NdeI 3059 CATATG CA^TATG PacI 1321 TTAATTAA TTAAT^TAA PmaCI 3509 CACGTG CAC^GTG PmlI 3509 CACGTG CAC^GTG PpuMI 7093 GGGACCC [GA]G^G[AT]CC[CT] SacI 6376 GAGCTC GAGCT^C SapI 7270 GAAGAGC GCTCTTC(1/4) ScaI 4674 AGTACT AGT^ACT SexAI 4614 ACCTGGT A^CC[AT]GGT SpeI 739 ACTAGT A^CTAGT SspBI 823 TGTACA T^GTACA StuI 2828 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Alw44I 389 GTGCAC G^TGCAC 2251 GTGCAC AocI 880 CCTGAGG CC^T[ACGT]AGG 3519 CCTGAGG ApaLI 389 GTGCAC G^TGCAC 2251 GTGCAC AvrII 3201 CCTAGG C^CTAGG 6825 CCTAGG BalI 2639 TGGCCA TGG^CCA 6973 TGGCCA BanI 3514 GGTACC G^G[CT][GA]CC 6427 GGTGCC BbvCI 1016 CCTCAGC CCTCAGC(-5/-2) 7288 CCTCAGC BcnI 4230 CCCGG CC^[GC]GG 5409 CCGGG BlnI 3201 CCTAGG C^CTAGG 6825 CCTAGG BsaAI 406 TACGTG [CT]AC^GT[GA] 3509 CACGTG BsgI 143 CTGCAC GTGCAG(16/14) 2476 CTGCAC BspLU11I 4224 ACATGT A^CATGT 5702 ACATGT BstAPI 136 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2085 GCACTATTTGC BstBI 1106 TTCGAA TT^CGAA 1952 TTCGAA BstEII 3655 GGTGACC G^GT[ACGT]ACC 4233 GGTAACC BstPI 3655 GGTGACC G^GT[ACGT]ACC 4233 GGTAACC Bsu36I 880 CCTGAGG CC^T[ACGT]AGG 3519 CCTGAGG DraII 3557 GGGGCCT [GA]G^G[ACGT]CC[CT] 7093 GGGACCC DraIII 2245 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG 4613 CACCTGGTG EaeI 2639 TGGCCA [CT]^GGCC[GA] 6973 TGGCCA Eco81I 880 CCTGAGG CC^T[ACGT]AGG 3519 CCTGAGG EcoO109I 3557 GGGGCCT [GA]G^G[ACGT]CC[CT] 7093 GGGACCC EcoO65I 3655 GGTGACC G^GT[ACGT]ACC 4233 GGTAACC EcoRI 4079 GAATTC G^AATTC 6479 GAATTC EcoT22I 7324 ATGCAT ATGCA^T 7410 ATGCAT HgaI 1435 GCGTC GACGC(5/10) 4097 GCGTC HpaI 2863 GTTAAC GTT^AAC 5686 GTTAAC MluNI 2639 TGGCCA TGG^CCA 6973 TGGCCA MscI 2639 TGGCCA TGG^CCA 6973 TGGCCA NciI 4230 CCCGG CC^[GC]GG 5409 CCGGG NsiI 7324 ATGCAT ATGCA^T 7410 ATGCAT NspV 1106 TTCGAA TT^CGAA 1952 TTCGAA PstI 4602 CTGCAG CTGCA^G 7068 CTGCAG SfuI 1106 TTCGAA TT^CGAA 1952 TTCGAA XcmI 2025 CCAGGCTGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3501 CCACTGGTCACGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaI AvaII BamHI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PaeR7I PleI PshBI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoI XhoII XmnI