##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AhdI ApaI AscI AspEI
AspI BbeI BclI BlpI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BssSI Bst1107I
BstZ17I CelII CpoI DrdI EagI
Eam1105I EclXI Eco52I FbaI FseI
Hin1I KasI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI PflFI PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3514 GGTACC G^GTACC
AccII 4096 CGCG CG^CG
AflII 1260 CTTAAG C^TTAAG
Aor51HI 6806 AGCGCT AGC^GCT
Asp718I 3514 GGTACC G^GTACC
AviII 2336 TGCGCA TGC^GCA
BanII 6376 GAGCTC G[GA]GC[CT]^C
BbrPI 3509 CACGTG CAC^GTG
BcgI 6069 CGAAAATAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 7678 GGATAC GTATCC(6/5)
BfrI 1260 CTTAAG C^TTAAG
BglI 1378 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
Bsp1407I 823 TGTACA T^GTACA
BspDI 509 ATCGAT AT^CGAT
BspMI 2471 ACCTGC ACCTGC(4/8)
BsrFI 5342 ACCGGC [GA]^CCGG[CT]
BsrGI 823 TGTACA T^GTACA
BstUI 4096 CGCG CG^CG
Cfr10I 5342 ACCGGC [GA]^CCGG[CT]
ClaI 509 ATCGAT AT^CGAT
Eco47III 6806 AGCGCT AGC^GCT
EcoNI 1211 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2336 TGCGCA TGC^GCA
HaeII 6806 AGCGCT [GA]GCGC^[CT]
KpnI 3514 GGTACC GGTAC^C
MvnI 4096 CGCG CG^CG
NdeI 3059 CATATG CA^TATG
PacI 1321 TTAATTAA TTAAT^TAA
PmaCI 3509 CACGTG CAC^GTG
PmlI 3509 CACGTG CAC^GTG
PpuMI 7093 GGGACCC [GA]G^G[AT]CC[CT]
SacI 6376 GAGCTC GAGCT^C
SapI 7270 GAAGAGC GCTCTTC(1/4)
ScaI 4674 AGTACT AGT^ACT
SexAI 4614 ACCTGGT A^CC[AT]GGT
SpeI 739 ACTAGT A^CTAGT
SspBI 823 TGTACA T^GTACA
StuI 2828 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 389 GTGCAC G^TGCAC
2251 GTGCAC
AocI 880 CCTGAGG CC^T[ACGT]AGG
3519 CCTGAGG
ApaLI 389 GTGCAC G^TGCAC
2251 GTGCAC
AvrII 3201 CCTAGG C^CTAGG
6825 CCTAGG
BalI 2639 TGGCCA TGG^CCA
6973 TGGCCA
BanI 3514 GGTACC G^G[CT][GA]CC
6427 GGTGCC
BbvCI 1016 CCTCAGC CCTCAGC(-5/-2)
7288 CCTCAGC
BcnI 4230 CCCGG CC^[GC]GG
5409 CCGGG
BlnI 3201 CCTAGG C^CTAGG
6825 CCTAGG
BsaAI 406 TACGTG [CT]AC^GT[GA]
3509 CACGTG
BsgI 143 CTGCAC GTGCAG(16/14)
2476 CTGCAC
BspLU11I 4224 ACATGT A^CATGT
5702 ACATGT
BstAPI 136 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2085 GCACTATTTGC
BstBI 1106 TTCGAA TT^CGAA
1952 TTCGAA
BstEII 3655 GGTGACC G^GT[ACGT]ACC
4233 GGTAACC
BstPI 3655 GGTGACC G^GT[ACGT]ACC
4233 GGTAACC
Bsu36I 880 CCTGAGG CC^T[ACGT]AGG
3519 CCTGAGG
DraII 3557 GGGGCCT [GA]G^G[ACGT]CC[CT]
7093 GGGACCC
DraIII 2245 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
4613 CACCTGGTG
EaeI 2639 TGGCCA [CT]^GGCC[GA]
6973 TGGCCA
Eco81I 880 CCTGAGG CC^T[ACGT]AGG
3519 CCTGAGG
EcoO109I 3557 GGGGCCT [GA]G^G[ACGT]CC[CT]
7093 GGGACCC
EcoO65I 3655 GGTGACC G^GT[ACGT]ACC
4233 GGTAACC
EcoRI 4079 GAATTC G^AATTC
6479 GAATTC
EcoT22I 7324 ATGCAT ATGCA^T
7410 ATGCAT
HgaI 1435 GCGTC GACGC(5/10)
4097 GCGTC
HpaI 2863 GTTAAC GTT^AAC
5686 GTTAAC
MluNI 2639 TGGCCA TGG^CCA
6973 TGGCCA
MscI 2639 TGGCCA TGG^CCA
6973 TGGCCA
NciI 4230 CCCGG CC^[GC]GG
5409 CCGGG
NsiI 7324 ATGCAT ATGCA^T
7410 ATGCAT
NspV 1106 TTCGAA TT^CGAA
1952 TTCGAA
PstI 4602 CTGCAG CTGCA^G
7068 CTGCAG
SfuI 1106 TTCGAA TT^CGAA
1952 TTCGAA
XcmI 2025 CCAGGCTGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3501 CCACTGGTCACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BamHI
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PleI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoI XhoII
XmnI