##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI AspEI
AspI BclI BfrI BsiWI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SfuI SgrAI
SnaBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1939 GGTACC G^GTACC
AccIII 3763 TCCGGA T^CCGGA
AcsI 5856 AAATTC [GA]^AATT[CT]
AflIII 5206 ACATGT A^C[GA][CT]GT
AocI 5109 CCTGAGG CC^T[ACGT]AGG
Aor51HI 6331 AGCGCT AGC^GCT
ApoI 5856 AAATTC [GA]^AATT[CT]
Asp718I 1939 GGTACC G^GTACC
AviII 466 TGCGCA TGC^GCA
AvrII 4541 CCTAGG C^CTAGG
BbrPI 5313 CACGTG CAC^GTG
BciVI 3581 GGATAC GTATCC(6/5)
BlnI 4541 CCTAGG C^CTAGG
BsaAI 5313 CACGTG [CT]AC^GT[GA]
BsaBI 464 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3763 TCCGGA T^CCGGA
BsmBI 4611 GAGACG CGTCTC(1/5)
BspDI 4137 ATCGAT AT^CGAT
BspEI 3763 TCCGGA T^CCGGA
BspLU11I 5206 ACATGT A^CATGT
BsrDI 1437 GCAATG GCAATG(2/0)
BssHII 2256 GCGCGC G^CGCGC
BssSI 1473 CTCGTG CACGAG(-5/-1)
Bsu36I 5109 CCTGAGG CC^T[ACGT]AGG
ClaI 4137 ATCGAT AT^CGAT
Eco47III 6331 AGCGCT AGC^GCT
Eco81I 5109 CCTGAGG CC^T[ACGT]AGG
FspI 466 TGCGCA TGC^GCA
KpnI 1939 GGTACC GGTAC^C
MamI 464 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3763 TCCGGA T^CCGGA
NaeI 6650 GCCGGC GCC^GGC
NdeI 5737 CATATG CA^TATG
NgoMIV 6650 GCCGGC G^CCGGC
NheI 1070 GCTAGC G^CTAGC
PmaCI 5313 CACGTG CAC^GTG
PmlI 5313 CACGTG CAC^GTG
ScaI 4906 AGTACT AGT^ACT
SexAI 2943 ACCAGGT A^CC[AT]GGT
SpeI 1304 ACTAGT A^CTAGT
Tsp509I 5857 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
Alw44I 3009 GTGCAC G^TGCAC
5652 GTGCAC
ApaLI 3009 GTGCAC G^TGCAC
5652 GTGCAC
BamHI 93 GGATCC G^GATCC
3760 GGATCC
BglII 2116 AGATCT A^GATCT
2227 AGATCT
BsaWI 1426 ACCGGA [AT]^CCGG[AT]
3763 TCCGGA
BspHI 4690 TCATGA T^CATGA
5965 TCATGA
BstXI 2875 CCAACAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6805 CCAAGAACCTGG
KspI 4737 CCGCGG CCGC^GG
6657 CCGCGG
RcaI 4690 TCATGA T^CATGA
5965 TCATGA
SacII 4737 CCGCGG CCGC^GG
6657 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI SphI Sse8387I
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI XmnI