##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AviII AvrII
BbrPI BciVI BfrI BlnI BsiEI
BsiWI BspDI BsrFI BssHII BstBI
Cfr10I ClaI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FseI FspI
HpaI KspI MluI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI SacI SacII
SalI SbfI SfiI SfuI SgrAI
SpeI SphI Sse8387I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 3306 TCCGGA T^CCGGA
AcyI 446 GGCGCC G[GA]^CG[CT]C
ApaI 4897 GGGCCC GGGCC^C
BamHI 5793 GGATCC G^GATCC
BbeI 446 GGCGCC GGCGC^C
BbvCI 2262 GCTGAGG CCTCAGC(-5/-2)
BcgI 494 GCAACGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 4296 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 446 GGCGCC G[GA]^CG[CT]C
BsaWI 3306 TCCGGA [AT]^CCGG[AT]
BseAI 3306 TCCGGA T^CCGGA
BsmBI 1490 CGTCTC CGTCTC(1/5)
BspEI 3306 TCCGGA T^CCGGA
BspHI 6710 TCATGA T^CATGA
BssSI 2146 CACGAG CACGAG(-5/-1)
BstEII 5343 GGTGACC G^GT[ACGT]ACC
BstPI 5343 GGTGACC G^GT[ACGT]ACC
CpoI 3330 CGGACCG CG^G[AT]CCG
EcoO65I 5343 GGTGACC G^GT[ACGT]ACC
EcoRV 4034 GATATC GAT^ATC
Hin1I 446 GGCGCC G[GA]^CG[CT]C
HincII 1630 GTCAAC GT[CT]^[GA]AC
HindII 1630 GTCAAC GT[CT]^[GA]AC
KasI 446 GGCGCC G^GCGCC
MamI 4296 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3306 TCCGGA T^CCGGA
NarI 446 GGCGCC GG^CGCC
NdeI 849 CATATG CA^TATG
NheI 645 GCTAGC G^CTAGC
RcaI 6710 TCATGA T^CATGA
RsrII 3330 CGGACCG CG^G[AT]CCG
SfoI 446 GGCGCC GGC^GCC
SmaI 2286 CCCGGG CCC^GGG
SnaBI 4400 TACGTA TAC^GTA
SspI 1670 AATATT AAT^ATT
SwaI 3655 ATTTAAAT ATTT^AAAT
XmaCI 2286 CCCGGG C^CCGGG
XmaI 2286 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 96 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2753 GACTCTGAGTC
AseI 1667 ATTAAT AT^TAAT
2808 ATTAAT
AsnI 1667 ATTAAT AT^TAAT
2808 ATTAAT
AspEI 96 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2753 GACTCTGAGTC
AspI 2353 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5513 GACATTGTC
BlpI 664 GCTAAGC GC^T[ACGT]AGC
6368 GCTCAGC
Bpu1102I 664 GCTAAGC GC^T[ACGT]AGC
6368 GCTCAGC
BsaAI 601 TACGTG [CT]AC^GT[GA]
4400 TACGTA
BsmI 6849 GAATGC GAATGC(1/-1)
4809 GCATTC
Bsp1407I 2416 TGTACA T^GTACA
4389 TGTACA
BsrBI 4278 CCGCTC CCGCTC(-3/-3)
5070 GAGCGG
BsrDI 4214 CATTGC GCAATG(2/0)
5508 GCAATG
BsrGI 2416 TGTACA T^GTACA
4389 TGTACA
Bst1107I 119 GTATAC GTA^TAC
3446 GTATAC
BstAPI 2294 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3866 GCAACAAGTGC
BstZ17I 119 GTATAC GTA^TAC
3446 GTATAC
CelII 664 GCTAAGC GC^T[ACGT]AGC
6368 GCTCAGC
DraIII 477 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
6993 CACATGGTG
DrdI 3829 GACAAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6799 GACTGGAAGGTC
Eam1105I 96 GACCCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2753 GACTCTGAGTC
MfeI 1085 CAATTG C^AATTG
3427 CAATTG
MunI 1085 CAATTG C^AATTG
3427 CAATTG
NcoI 4568 CCATGG C^CATGG
6917 CCATGG
PflFI 2353 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5513 GACATTGTC
PflMI 4877 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5422 CCAGTTAGTGG
PshBI 1667 ATTAAT AT^TAAT
2808 ATTAAT
PvuII 3215 CAGCTG CAG^CTG
3784 CAGCTG
SexAI 4882 ACCTGGT A^CC[AT]GGT
6681 ACCAGGT
SspBI 2416 TGTACA T^GTACA
4389 TGTACA
Tth111I 2353 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5513 GACATTGTC
Van91I 4877 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5422 CCAGTTAGTGG
XbaI 256 TCTAGA T^CTAGA
2568 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI