##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
Acc65I AccI AccIII AflII AscI
Asp718I AvrII BciVI BfrI BglII
BlnI BsaBI BseAI BspDI BspEI
BsrBI BssHII Bst1107I BstZ17I ClaI
CpoI EcoRI EcoRV FseI HpaI
KpnI MamI MfeI MluI MroI
MunI NotI NruI PacI PmeI
PshAI PvuI RsrII SalI ScaI
SfiI SgrAI SnaBI SpeI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3174 GACGTC GACGT^C
AgeI 5184 ACCGGT A^CCGGT
Asp700I 5483 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2854 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 747 TGCGCA TGC^GCA
BamHI 2838 GGATCC G^GATCC
BcgI 1814 CGAGCACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3521 TGATCA T^GATCA
BsmI 1135 GAATGC GAATGC(1/-1)
BssSI 3057 CACGAG CACGAG(-5/-1)
BstBI 5586 TTCGAA TT^CGAA
BstXI 2013 CCATGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 223 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4325 CGGCCG C^GGCCG
EclXI 4325 CGGCCG C^GGCCG
Eco52I 4325 CGGCCG C^GGCCG
FbaI 3521 TGATCA T^GATCA
FspI 747 TGCGCA TGC^GCA
HindIII 6586 AAGCTT A^AGCTT
NdeI 2209 CATATG CA^TATG
NheI 2796 GCTAGC G^CTAGC
NspV 5586 TTCGAA TT^CGAA
PflFI 2854 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5184 ACCGGT A^CCGGT
SbfI 5241 CCTGCAGG CCTGCA^GG
SfuI 5586 TTCGAA TT^CGAA
SmaI 4299 CCCGGG CCC^GGG
Sse8387I 5241 CCTGCAGG CCTGCA^GG
SwaI 6861 ATTTAAAT ATTT^AAAT
Tth111I 2854 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 594 TCTAGA T^CTAGA
XmaCI 4299 CCCGGG C^CCGGG
XmaI 4299 CCCGGG C^CCGGG
XmnI 5483 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 2644 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5173 GACGAGCTGTC
Aor51HI 3416 AGCGCT AGC^GCT
4783 AGCGCT
AseI 6967 ATTAAT AT^TAAT
6994 ATTAAT
AsnI 6967 ATTAAT AT^TAAT
6994 ATTAAT
AspEI 2644 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5173 GACGAGCTGTC
BbrPI 1762 CACGTG CAC^GTG
2632 CACGTG
BbvCI 3507 CCTCAGC CCTCAGC(-5/-2)
1266 GCTGAGG
BglI 387 GCCAACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3772 GCCCGCAAGGC
BlpI 2936 GCTTAGC GC^T[ACGT]AGC
4779 GCTGAGC
Bpu1102I 2936 GCTTAGC GC^T[ACGT]AGC
4779 GCTGAGC
BsaAI 1762 CACGTG [CT]AC^GT[GA]
2632 CACGTG
BsiEI 4325 CGGCCG CG[GA][CT]^CG
4938 CGACCG
BsiWI 3305 CGTACG C^GTACG
3707 CGTACG
BstEII 3852 GGTCACC G^GT[ACGT]ACC
4710 GGTCACC
BstPI 3852 GGTCACC G^GT[ACGT]ACC
4710 GGTCACC
CelII 2936 GCTTAGC GC^T[ACGT]AGC
4779 GCTGAGC
Eam1105I 2644 GACGGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5173 GACGAGCTGTC
Eco47III 3416 AGCGCT AGC^GCT
4783 AGCGCT
EcoO65I 3852 GGTCACC G^GT[ACGT]ACC
4710 GGTCACC
HincII 166 GTTGAC GT[CT]^[GA]AC
4492 GTCAAC
HindII 166 GTTGAC GT[CT]^[GA]AC
4492 GTCAAC
KspI 4013 CCGCGG CCGC^GG
5798 CCGCGG
NcoI 1396 CCATGG C^CATGG
4457 CCATGG
PmaCI 1762 CACGTG CAC^GTG
2632 CACGTG
PmlI 1762 CACGTG CAC^GTG
2632 CACGTG
PshBI 6967 ATTAAT AT^TAAT
6994 ATTAAT
SacI 3000 GAGCTC GAGCT^C
4533 GAGCTC
SacII 4013 CCGCGG CCGC^GG
5798 CCGCGG
SexAI 2172 ACCAGGT A^CC[AT]GGT
6147 ACCTGGT
SphI 4883 GCATGC GCATG^C
6338 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccII AciI AclI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NsiI NspI PaeR7I PflMI PleI
PpuMI Psp1406I PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII