##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AccIII AflII AscI Asp718I
AviII AvrII BamHI BfrI BglII
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI FseI FspI HpaI KpnI
MamI MroI NdeI NheI NotI
NspV PmeI PvuI SacI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3556 GACGTC GACGT^C
AclI 1372 AACGTT AA^CGTT
AgeI 1846 ACCGGT A^CCGGT
AseI 796 ATTAAT AT^TAAT
AsnI 796 ATTAAT AT^TAAT
BbrPI 5227 CACGTG CAC^GTG
BciVI 7187 GGATAC GTATCC(6/5)
BclI 4557 TGATCA T^GATCA
BsaWI 1846 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4376 TGTACA T^GTACA
BsrGI 4376 TGTACA T^GTACA
BstEII 2865 GGTGACC G^GT[ACGT]ACC
BstPI 2865 GGTGACC G^GT[ACGT]ACC
CpoI 225 CGGACCG CG^G[AT]CCG
EcoO65I 2865 GGTGACC G^GT[ACGT]ACC
EcoT22I 3958 ATGCAT ATGCA^T
FbaI 4557 TGATCA T^GATCA
HindIII 3628 AAGCTT A^AGCTT
KspI 6538 CCGCGG CCGC^GG
MluI 5432 ACGCGT A^CGCGT
NsiI 3958 ATGCAT ATGCA^T
PacI 793 TTAATTAA TTAAT^TAA
PaeR7I 906 CTCGAG C^TCGAG
PinAI 1846 ACCGGT A^CCGGT
PmaCI 5227 CACGTG CAC^GTG
PmlI 5227 CACGTG CAC^GTG
PshBI 796 ATTAAT AT^TAAT
Psp1406I 1372 AACGTT AA^CGTT
RsrII 225 CGGACCG CG^G[AT]CCG
SacII 6538 CCGCGG CCGC^GG
SalI 5038 GTCGAC G^TCGAC
SspBI 4376 TGTACA T^GTACA
SspI 2036 AATATT AAT^ATT
XhoI 906 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 5038 GTCGAC GT^[AC][GT]AC
5200 GTCTAC
AocI 1594 CCTCAGG CC^T[ACGT]AGG
2602 CCTCAGG
Aor51HI 5408 AGCGCT AGC^GCT
6283 AGCGCT
Asp700I 2478 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3804 GAAGTGTTTC
BsiEI 164 CGGCCG CG[GA][CT]^CG
6227 CGGCCG
BspHI 827 TCATGA T^CATGA
6608 TCATGA
BspLU11I 1872 ACATGT A^CATGT
2006 ACATGT
BsrDI 4543 CATTGC GCAATG(2/0)
7309 GCAATG
BssSI 4365 CACGAG CACGAG(-5/-1)
2312 CTCGTG
Bsu36I 1594 CCTCAGG CC^T[ACGT]AGG
2602 CCTCAGG
DraIII 5224 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
5250 CACTGTGTG
DrdI 5191 GACCGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6306 GACCCTGAAGTC
EagI 164 CGGCCG C^GGCCG
6227 CGGCCG
EclXI 164 CGGCCG C^GGCCG
6227 CGGCCG
Eco47III 5408 AGCGCT AGC^GCT
6283 AGCGCT
Eco52I 164 CGGCCG C^GGCCG
6227 CGGCCG
Eco81I 1594 CCTCAGG CC^T[ACGT]AGG
2602 CCTCAGG
EcoNI 930 CCTAACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5664 CCTGCAGCAGG
EcoRV 1141 GATATC GAT^ATC
6207 GATATC
MfeI 1220 CAATTG C^AATTG
1457 CAATTG
MunI 1220 CAATTG C^AATTG
1457 CAATTG
NruI 189 TCGCGA TCG^CGA
3756 TCGCGA
PshAI 138 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2207 GACCCCTGTC
RcaI 827 TCATGA T^CATGA
6608 TCATGA
SfiI 496 GGCCTGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6219 GGCCCAGGCGGCC
SgrAI 1845 CACCGGTG C[GA]^CCGG[CT]G
5686 CGCCGGCG
XmnI 2478 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3804 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI