##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
Acc65I AccIII AflII AscI Asp718I
AviII AvrII BamHI BfrI BglII
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstZ17I
ClaI FseI FspI HpaI KpnI
MamI MroI NdeI NheI NotI
NspV PmeI PvuI SacI SbfI
ScaI SfuI SnaBI SpeI Sse8387I
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3670 GACGTC GACGT^C
AclI 1372 AACGTT AA^CGTT
AgeI 1960 ACCGGT A^CCGGT
AseI 796 ATTAAT AT^TAAT
AsnI 796 ATTAAT AT^TAAT
BbrPI 5341 CACGTG CAC^GTG
BciVI 7301 GGATAC GTATCC(6/5)
BclI 4671 TGATCA T^GATCA
BsaWI 1960 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4490 TGTACA T^GTACA
BsrGI 4490 TGTACA T^GTACA
BstEII 2979 GGTGACC G^GT[ACGT]ACC
BstPI 2979 GGTGACC G^GT[ACGT]ACC
CpoI 225 CGGACCG CG^G[AT]CCG
EcoO65I 2979 GGTGACC G^GT[ACGT]ACC
EcoT22I 4072 ATGCAT ATGCA^T
FbaI 4671 TGATCA T^GATCA
HindIII 3742 AAGCTT A^AGCTT
KspI 6652 CCGCGG CCGC^GG
MluI 5546 ACGCGT A^CGCGT
NsiI 4072 ATGCAT ATGCA^T
PacI 793 TTAATTAA TTAAT^TAA
PaeR7I 906 CTCGAG C^TCGAG
PinAI 1960 ACCGGT A^CCGGT
PmaCI 5341 CACGTG CAC^GTG
PmlI 5341 CACGTG CAC^GTG
PshBI 796 ATTAAT AT^TAAT
Psp1406I 1372 AACGTT AA^CGTT
RsrII 225 CGGACCG CG^G[AT]CCG
SacII 6652 CCGCGG CCGC^GG
SalI 5152 GTCGAC G^TCGAC
SspBI 4490 TGTACA T^GTACA
SspI 2150 AATATT AAT^ATT
XhoI 906 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 5152 GTCGAC GT^[AC][GT]AC
5314 GTCTAC
AocI 1708 CCTCAGG CC^T[ACGT]AGG
2716 CCTCAGG
Aor51HI 5522 AGCGCT AGC^GCT
6397 AGCGCT
Asp700I 2592 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAAGTGTTTC
BsiEI 164 CGGCCG CG[GA][CT]^CG
6341 CGGCCG
BspHI 827 TCATGA T^CATGA
6722 TCATGA
BspLU11I 1986 ACATGT A^CATGT
2120 ACATGT
BsrDI 4657 CATTGC GCAATG(2/0)
7423 GCAATG
BssSI 4479 CACGAG CACGAG(-5/-1)
2426 CTCGTG
Bsu36I 1708 CCTCAGG CC^T[ACGT]AGG
2716 CCTCAGG
DraIII 5338 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
5364 CACTGTGTG
EagI 164 CGGCCG C^GGCCG
6341 CGGCCG
EclXI 164 CGGCCG C^GGCCG
6341 CGGCCG
Eco47III 5522 AGCGCT AGC^GCT
6397 AGCGCT
Eco52I 164 CGGCCG C^GGCCG
6341 CGGCCG
Eco81I 1708 CCTCAGG CC^T[ACGT]AGG
2716 CCTCAGG
EcoNI 930 CCTAACGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5778 CCTGCAGCAGG
EcoRV 1141 GATATC GAT^ATC
6321 GATATC
MfeI 1220 CAATTG C^AATTG
1571 CAATTG
MunI 1220 CAATTG C^AATTG
1571 CAATTG
NruI 189 TCGCGA TCG^CGA
3870 TCGCGA
PshAI 138 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2321 GACCCCTGTC
RcaI 827 TCATGA T^CATGA
6722 TCATGA
SfiI 496 GGCCTGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6333 GGCCCAGGCGGCC
SgrAI 1959 CACCGGTG C[GA]^CCGG[CT]G
5800 CGCCGGCG
XmnI 2592 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3918 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI ApaI ApaLI ApoI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI Eam1105I EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI