##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI AviII BamHI BbrPI
BcgI BglI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BsrFI BssSI
BstBI BstEII BstPI Cfr10I ClaI
CpoI DrdI EcoO65I EcoRV FseI
FspI KspI MamI MluI MroI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SphI Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 275 GGTACC G^GTACC
AflII 6852 CTTAAG C^TTAAG
AhdI 3391 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4058 AGCGCT AGC^GCT
Asp718I 275 GGTACC G^GTACC
AspEI 3391 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3763 GAGCAC G[AT]GC[AT]^C
AvrII 7079 CCTAGG C^CTAGG
BbeI 7434 GGCGCC GGCGC^C
BciVI 5387 GGATAC GTATCC(6/5)
BclI 776 TGATCA T^GATCA
BfrI 6852 CTTAAG C^TTAAG
BglII 7018 AGATCT A^GATCT
BlnI 7079 CCTAGG C^CTAGG
BlpI 2263 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2263 GCTCAGC GC^T[ACGT]AGC
BsaAI 4136 CACGTA [CT]AC^GT[GA]
BsiHKAI 3763 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1293 TGTACA T^GTACA
BspHI 7344 TCATGA T^CATGA
BsrGI 1293 TGTACA T^GTACA
BssHII 164 GCGCGC G^CGCGC
Bst1107I 1364 GTATAC GTA^TAC
BstAPI 1370 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1364 GTATAC GTA^TAC
CelII 2263 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3391 GACATTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4058 AGCGCT AGC^GCT
FbaI 776 TGATCA T^GATCA
KasI 7434 GGCGCC G^GCGCC
KpnI 275 GGTACC GGTAC^C
MfeI 5575 CAATTG C^AATTG
MunI 5575 CAATTG C^AATTG
NarI 7434 GGCGCC GG^CGCC
NdeI 842 CATATG CA^TATG
RcaI 7344 TCATGA T^CATGA
SfoI 7434 GGCGCC GGC^GCC
SpeI 805 ACTAGT A^CTAGT
SspBI 1293 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 648 GTCTAC GT^[AC][GT]AC
1364 GTATAC
AocI 3308 CCTTAGG CC^T[ACGT]AGG
7279 CCTCAGG
AspI 5396 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7553 GACTCCGTC
BalI 423 TGGCCA TGG^CCA
2009 TGGCCA
BsiEI 205 CGGCCG CG[GA][CT]^CG
234 CGGCCG
BsrBI 3369 CCGCTC CCGCTC(-3/-3)
313 GAGCGG
Bsu36I 3308 CCTTAGG CC^T[ACGT]AGG
7279 CCTCAGG
EagI 205 CGGCCG C^GGCCG
234 CGGCCG
EclXI 205 CGGCCG C^GGCCG
234 CGGCCG
Eco52I 205 CGGCCG C^GGCCG
234 CGGCCG
Eco81I 3308 CCTTAGG CC^T[ACGT]AGG
7279 CCTCAGG
EcoNI 612 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4560 CCTTAGAGAGG
EcoT22I 1974 ATGCAT ATGCA^T
4271 ATGCAT
HpaI 4131 GTTAAC GTT^AAC
4839 GTTAAC
MluNI 423 TGGCCA TGG^CCA
2009 TGGCCA
MscI 423 TGGCCA TGG^CCA
2009 TGGCCA
NcoI 2168 CCATGG C^CATGG
4799 CCATGG
NsiI 1974 ATGCAT ATGCA^T
4271 ATGCAT
PflFI 5396 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7553 GACTCCGTC
ScaI 2341 AGTACT AGT^ACT
3316 AGTACT
SmaI 106 CCCGGG CCC^GGG
7486 CCCGGG
StuI 3281 AGGCCT AGG^CCT
5763 AGGCCT
Tth111I 5396 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7553 GACTCCGTC
XbaI 2799 TCTAGA T^CTAGA
7132 TCTAGA
XmaCI 106 CCCGGG C^CCGGG
7486 CCCGGG
XmaI 106 CCCGGG C^CCGGG
7486 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AseI AsnI Asp700I AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI