##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcsI 583 GAATTC [GA]^AATT[CT]
AfaI 1102 GTAC GT^AC
AflIII 602 ACATGT A^C[GA][CT]GT
ApoI 583 GAATTC [GA]^AATT[CT]
AseI 325 ATTAAT AT^TAAT
AsnI 325 ATTAAT AT^TAAT
Asp700I 648 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 383 GGTCC G^G[AT]CC
BamHI 1111 GGATCC G^GATCC
BanII 893 GGGCTC G[GA]GC[CT]^C
BbsI 231 GAAGAC GAAGAC(2/6)
BciVI 715 GTATCC GTATCC(6/5)
BcnI 1090 CCGGG CC^[GC]GG
BfaI 1012 CTAG C^TAG
BlpI 14 GCTGAGC GC^T[ACGT]AGC
BmyI 893 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 14 GCTGAGC GC^T[ACGT]AGC
BpuAI 231 GAAGAC GAAGAC(2/6)
BsaI 616 GAGACC GGTCTC(1/5)
BsgI 1120 CTGCAC GTGCAG(16/14)
BsmAI 616 GAGAC GTCTC(1/5)
BsmI 1073 GCATTC GAATGC(1/-1)
Bsp1286I 893 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 602 ACATGT A^CATGT
BspMI 364 GCAGGT ACCTGC(4/8)
BsrI 979 CCAGT ACTGG(1/-1)
BstF5I 113 CATCC GGATG(2/0)
CelII 14 GCTGAGC GC^T[ACGT]AGC
DraI 1053 TTTAAA TTT^AAA
DraII 925 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 652 CTCTTC CTCTTC(1/4)
EcoNI 730 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 925 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 583 GAATTC G^AATTC
FokI 113 CATCC GGATG(9/13)
HaeII 409 AGCGCC [GA]GCGC^[CT]
HapII 1090 CCGG C^CGG
HpaII 1090 CCGG C^CGG
Ksp632I 652 CTCTTC CTCTTC(1/4)
MaeI 1012 CTAG C^TAG
MaeII 235 ACGT A^CGT
MfeI 290 CAATTG C^AATTG
MspI 1090 CCGG C^CGG
MunI 290 CAATTG C^AATTG
NciI 1090 CCGGG CC^[GC]GG
PflMI 56 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 325 ATTAAT AT^TAAT
PstI 1028 CTGCAG CTGCA^G
RsaI 1102 GTAC GT^AC
SphI 667 GCATGC GCATG^C
TaqI 931 TCGA T^CGA
Tsp45I 99 GTGAC ^GT[GC]AC
TthHB8I 931 TCGA T^CGA
Van91I 56 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 979 CCAGTCTGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 648 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 44 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
809 CAGCAACTG
BbvCI 620 CCTCAGC CCTCAGC(-5/-2)
783 GCTGAGG
BglII 350 AGATCT A^GATCT
844 AGATCT
BsmFI 46 GGGAC GGGAC(10/14)
356 GGGAC
BsrBI 2 CCGCTC CCGCTC(-3/-3)
867 CCGCTC
CfoI 410 GCGC GCG^C
1071 GCGC
FauI 866 CCCGC CCCGC(4/6)
480 GCGGG
HhaI 410 GCGC GCG^C
1071 GCGC
HinP1I 410 GCGC G^CGC
1071 GCGC
MaeIII 99 GTGAC ^GT[ACGT]AC
398 GTTAC
MslI 513 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
699 CACACTTGTG
MspA1I 12 CAGCTG C[AC]G^C[GT]G
158 CAGCTG
MwoI 903 GCTTACAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
912 GCATGGCAGGC
PvuII 12 CAGCTG CAG^CTG
158 CAGCTG
SfaNI 834 GATGC GCATC(5/9)
746 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwI BbvI BpmI
BsaJI BsiYI BslI BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII