##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AvrII BbeI BciVI BfrI BlnI
BlpI Bpu1102I BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI Eco47III FseI HindIII HpaI
KasI KspI MfeI MluI MroI
MunI NarI NheI NotI NruI
NspV PacI PinAI PmeI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccII 7781 CGCG CG^CG
AseI 6349 ATTAAT AT^TAAT
AsnI 6349 ATTAAT AT^TAAT
AviII 1715 TGCGCA TGC^GCA
BamHI 4258 GGATCC G^GATCC
BsaBI 5185 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 484 ACCGGA [AT]^CCGG[AT]
BsrBI 3589 CCGCTC CCGCTC(-3/-3)
BsrDI 6008 GCAATG GCAATG(2/0)
BstAPI 1477 GCAAACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3945 GGTGACC G^GT[ACGT]ACC
BstPI 3945 GGTGACC G^GT[ACGT]ACC
BstUI 7781 CGCG CG^CG
DraIII 5636 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3945 GGTGACC G^GT[ACGT]ACC
EcoT22I 2993 ATGCAT ATGCA^T
FspI 1715 TGCGCA TGC^GCA
HaeII 2938 AGCGCC [GA]GCGC^[CT]
MamI 5185 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 7781 CGCG CG^CG
NaeI 37 GCCGGC GCC^GGC
NgoMIV 37 GCCGGC G^CCGGC
NsiI 2993 ATGCAT ATGCA^T
PflMI 1281 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 6349 ATTAAT AT^TAAT
SpeI 4396 ACTAGT A^CTAGT
SspI 676 AATATT AAT^ATT
Van91I 1281 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1372 GGTACC G^GTACC
7340 GGTACC
AcyI 108 GGCGTC G[GA]^CG[CT]C
3888 GACGCC
AflIII 2925 ACACGT A^C[GA][CT]GT
4667 ACACGT
AhdI 4201 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5707 GACTATGAGTC
Asp718I 1372 GGTACC G^GTACC
7340 GGTACC
AspEI 4201 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5707 GACTATGAGTC
AvaI 1036 CTCGAG C^[CT]CG[GA]G
3461 CTCGAG
BbrPI 1762 CACGTG CAC^GTG
6977 CACGTG
BcgI 3192 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7027 GCATCCAAGTCG
BclI 380 TGATCA T^GATCA
425 TGATCA
BsaHI 108 GGCGTC G[GA]^CG[CT]C
3888 GACGCC
BsgI 6974 CTGCAC GTGCAG(16/14)
5063 GTGCAG
BsmBI 3464 GAGACG CGTCTC(1/5)
3496 GAGACG
BsoBI 1036 CTCGAG C^[CT]CG[GA]G
3461 CTCGAG
BspHI 3300 TCATGA T^CATGA
7314 TCATGA
BsrFI 37 GCCGGC [GA]^CCGG[CT]
2836 GCCGGT
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
2836 GCCGGT
DraI 2307 TTTAAA TTT^AAA
4582 TTTAAA
EagI 3 CGGCCG C^GGCCG
6563 CGGCCG
Eam1105I 4201 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5707 GACTATGAGTC
EclXI 3 CGGCCG C^GGCCG
6563 CGGCCG
Eco52I 3 CGGCCG C^GGCCG
6563 CGGCCG
EcoRV 3420 GATATC GAT^ATC
5392 GATATC
FbaI 380 TGATCA T^GATCA
425 TGATCA
Hin1I 108 GGCGTC G[GA]^CG[CT]C
3888 GACGCC
HincII 3190 GTCGAC GT[CT]^[GA]AC
5033 GTCGAC
HindII 3190 GTCGAC GT[CT]^[GA]AC
5033 GTCGAC
KpnI 1372 GGTACC GGTAC^C
7340 GGTACC
NdeI 4678 CATATG CA^TATG
5912 CATATG
PaeR7I 1036 CTCGAG C^TCGAG
3461 CTCGAG
PmaCI 1762 CACGTG CAC^GTG
6977 CACGTG
PmlI 1762 CACGTG CAC^GTG
6977 CACGTG
RcaI 3300 TCATGA T^CATGA
7314 TCATGA
SalI 3190 GTCGAC G^TCGAC
5033 GTCGAC
SapI 1876 GAAGAGC GCTCTTC(1/4)
3807 GAAGAGC
SexAI 5637 ACCTGGT A^CC[AT]GGT
5680 ACCAGGT
SphI 4767 GCATGC GCATG^C
7432 GCATGC
XbaI 1566 TCTAGA T^CTAGA
5983 TCTAGA
XhoI 1036 CTCGAG C^TCGAG
3461 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AspI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmnI