##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AccIII AgeI AscI AviII BcgI
BciVI BglI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI Bst1107I BstZ17I
CelII ClaI CpoI DrdI EcoT22I
FseI FspI HindIII HpaI MfeI
MroI MunI NotI NruI NsiI
PacI PinAI PmeI PvuI RsrII
SfiI SgrAI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 6840 GACGTC GACGT^C
AclI 1145 AACGTT AA^CGTT
AocI 2247 CCTTAGG CC^T[ACGT]AGG
AvrII 1667 CCTAGG C^CTAGG
BbeI 7005 GGCGCC GGCGC^C
BbrPI 6271 CACGTG CAC^GTG
BclI 3148 TGATCA T^GATCA
BlnI 1667 CCTAGG C^CTAGG
BsaBI 2358 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 7148 ACCGGA [AT]^CCGG[AT]
BsiEI 4413 CGGCCG CG[GA][CT]^CG
BsmBI 3996 GAGACG CGTCTC(1/5)
BssHII 3874 GCGCGC G^CGCGC
BssSI 2454 CACGAG CACGAG(-5/-1)
BstBI 3696 TTCGAA TT^CGAA
BstEII 571 GGTGACC G^GT[ACGT]ACC
BstPI 571 GGTGACC G^GT[ACGT]ACC
Bsu36I 2247 CCTTAGG CC^T[ACGT]AGG
EagI 4413 CGGCCG C^GGCCG
EclXI 4413 CGGCCG C^GGCCG
Eco52I 4413 CGGCCG C^GGCCG
Eco81I 2247 CCTTAGG CC^T[ACGT]AGG
EcoO65I 571 GGTGACC G^GT[ACGT]ACC
EcoRV 5797 GATATC GAT^ATC
FbaI 3148 TGATCA T^GATCA
KasI 7005 GGCGCC G^GCGCC
KspI 3890 CCGCGG CCGC^GG
MamI 2358 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 6535 ACGCGT A^CGCGT
NaeI 1167 GCCGGC GCC^GGC
NarI 7005 GGCGCC GG^CGCC
NdeI 2748 CATATG CA^TATG
NgoMIV 1167 GCCGGC G^CCGGC
NheI 6865 GCTAGC G^CTAGC
NspV 3696 TTCGAA TT^CGAA
PaeR7I 768 CTCGAG C^TCGAG
PmaCI 6271 CACGTG CAC^GTG
PmlI 6271 CACGTG CAC^GTG
PshAI 4453 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1145 AACGTT AA^CGTT
SacI 6773 GAGCTC GAGCT^C
SacII 3890 CCGCGG CCGC^GG
SalI 4502 GTCGAC G^TCGAC
SbfI 7071 CCTGCAGG CCTGCA^GG
SfoI 7005 GGCGCC GGC^GCC
SfuI 3696 TTCGAA TT^CGAA
SmaI 3883 CCCGGG CCC^GGG
SnaBI 719 TACGTA TAC^GTA
SpeI 131 ACTAGT A^CTAGT
Sse8387I 7071 CCTGCAGG CCTGCA^GG
StuI 4918 AGGCCT AGG^CCT
SwaI 138 ATTTAAAT ATTT^AAAT
XhoI 768 CTCGAG C^TCGAG
XmaCI 3883 CCCGGG C^CCGGG
XmaI 3883 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 6500 GGTACC G^GTACC
6793 GGTACC
AflII 3062 CTTAAG C^TTAAG
6073 CTTAAG
AhdI 554 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACCTGCGGTC
Alw44I 5365 GTGCAC G^TGCAC
6481 GTGCAC
Aor51HI 2742 AGCGCT AGC^GCT
5047 AGCGCT
ApaLI 5365 GTGCAC G^TGCAC
6481 GTGCAC
Asp718I 6500 GGTACC G^GTACC
6793 GGTACC
AspEI 554 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACCTGCGGTC
BbvCI 5964 CCTCAGC CCTCAGC(-5/-2)
4334 GCTGAGG
BfrI 3062 CTTAAG C^TTAAG
6073 CTTAAG
Bsp1407I 4989 TGTACA T^GTACA
7719 TGTACA
BspHI 5522 TCATGA T^CATGA
6102 TCATGA
BsrBI 1999 CCGCTC CCGCTC(-3/-3)
4329 GAGCGG
BsrDI 4485 CATTGC GCAATG(2/0)
6832 CATTGC
BsrGI 4989 TGTACA T^GTACA
7719 TGTACA
Eam1105I 554 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1475 GACCTGCGGTC
Eco47III 2742 AGCGCT AGC^GCT
5047 AGCGCT
EcoNI 364 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3955 CCTGAAAGAGG
KpnI 6500 GGTACC GGTAC^C
6793 GGTACC
NcoI 1552 CCATGG C^CATGG
3586 CCATGG
RcaI 5522 TCATGA T^CATGA
6102 TCATGA
SapI 2881 GAAGAGC GCTCTTC(1/4)
170 GCTCTTC
ScaI 6609 AGTACT AGT^ACT
6851 AGTACT
SphI 3606 GCATGC GCATG^C
6979 GCATGC
SspBI 4989 TGTACA T^GTACA
7719 TGTACA
SspI 1327 AATATT AAT^ATT
4840 AATATT
XcmI 6987 CCACCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7182 CCACTGCGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AseI AsnI Asp700I
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XbaI XhoII XmnI