##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp718I AspEI BamHI
BbeI BbrPI BsiEI BsiWI BspDI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FseI KasI KpnI
KspI MluI NaeI NarI NgoMIV
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3447 GTATAC GT^[AC][GT]AC
AcyI 4598 GGCGTC G[GA]^CG[CT]C
Aor51HI 4665 AGCGCT AGC^GCT
AvaI 4335 CTCGAG C^[CT]CG[GA]G
AviII 2232 TGCGCA TGC^GCA
BcgI 2714 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 90 TGATCA T^GATCA
BsaBI 6504 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4598 GGCGTC G[GA]^CG[CT]C
BsmBI 7220 GAGACG CGTCTC(1/5)
BsoBI 4335 CTCGAG C^[CT]CG[GA]G
Bsp1407I 5830 TGTACA T^GTACA
BsrGI 5830 TGTACA T^GTACA
Bst1107I 3447 GTATAC GTA^TAC
BstZ17I 3447 GTATAC GTA^TAC
DsaI 3738 CCATGG C^C[GA][CT]GG
Eco47III 4665 AGCGCT AGC^GCT
EcoNI 574 CCTATGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 90 TGATCA T^GATCA
FspI 2232 TGCGCA TGC^GCA
HaeII 4665 AGCGCT [GA]GCGC^[CT]
Hin1I 4598 GGCGTC G[GA]^CG[CT]C
HindIII 7070 AAGCTT A^AGCTT
MamI 6504 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 3738 CCATGG C^CATGG
NheI 4442 GCTAGC G^CTAGC
PaeR7I 4335 CTCGAG C^TCGAG
PflMI 2042 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 6002 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1299 GAGCTC GAGCT^C
SexAI 4931 ACCTGGT A^CC[AT]GGT
SspBI 5830 TGTACA T^GTACA
SwaI 5493 ATTTAAAT ATTT^AAAT
TaqI 4336 TCGA T^CGA
TthHB8I 4336 TCGA T^CGA
Van91I 2042 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 4335 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 5753 TCCGGA T^CCGGA
5772 TCCGGA
AseI 2159 ATTAAT AT^TAAT
5229 ATTAAT
AsnI 2159 ATTAAT AT^TAAT
5229 ATTAAT
Asp700I 1435 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6072 GAACTTTTTC
AspI 2066 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4930 GACCTGGTC
BalI 4303 TGGCCA TGG^CCA
5301 TGGCCA
BanI 847 GGTGCC G^G[CT][GA]CC
1699 GGCACC
BglI 71 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7172 GCCCCTTTGGC
BglII 4042 AGATCT A^GATCT
5333 AGATCT
BlpI 1490 GCTGAGC GC^T[ACGT]AGC
5005 GCTGAGC
Bpu1102I 1490 GCTGAGC GC^T[ACGT]AGC
5005 GCTGAGC
BseAI 5753 TCCGGA T^CCGGA
5772 TCCGGA
BspEI 5753 TCCGGA T^CCGGA
5772 TCCGGA
CelII 1490 GCTGAGC GC^T[ACGT]AGC
5005 GCTGAGC
EaeI 4303 TGGCCA [CT]^GGCC[GA]
5301 TGGCCA
EcoRI 1043 GAATTC G^AATTC
4990 GAATTC
EcoT22I 1710 ATGCAT ATGCA^T
2243 ATGCAT
HpaI 2952 GTTAAC GTT^AAC
3215 GTTAAC
MfeI 3986 CAATTG C^AATTG
6829 CAATTG
MluNI 4303 TGGCCA TGG^CCA
5301 TGGCCA
MroI 5753 TCCGGA T^CCGGA
5772 TCCGGA
MscI 4303 TGGCCA TGG^CCA
5301 TGGCCA
MunI 3986 CAATTG C^AATTG
6829 CAATTG
NsiI 1710 ATGCAT ATGCA^T
2243 ATGCAT
PflFI 2066 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4930 GACCTGGTC
PshBI 2159 ATTAAT AT^TAAT
5229 ATTAAT
ScaI 6851 AGTACT AGT^ACT
7077 AGTACT
Tth111I 2066 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4930 GACCTGGTC
XmnI 1435 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6072 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaII
AvrII BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BfrI BlnI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XcmI XhoII