##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
Acc65I AccIII AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
BamHI BbeI BbrPI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspEI BsrGI
BssHII BstBI CpoI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NgoMIV NotI NspV PacI PaeR7I
PmaCI PmeI PmlI PvuI RsrII
SacII SbfI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspBI SspI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 6847 GACGTC GACGT^C
AclI 4963 AACGTT AA^CGTT
AcyI 6847 GACGTC G[GA]^CG[CT]C
AflII 2684 CTTAAG C^TTAAG
AgeI 2623 ACCGGT A^CCGGT
AspI 7271 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 5605 GCAGAACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 7273 CCCGG CC^[GC]GG
BfrI 2684 CTTAAG C^TTAAG
BsaAI 5464 TACGTA [CT]AC^GT[GA]
BsaHI 6847 GACGTC G[GA]^CG[CT]C
BsaWI 2623 ACCGGT [AT]^CCGG[AT]
BspDI 5041 ATCGAT AT^CGAT
BssSI 3368 CTCGTG CACGAG(-5/-1)
Bst1107I 5093 GTATAC GTA^TAC
BstAPI 3029 GCATCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 5093 GTATAC GTA^TAC
ClaI 5041 ATCGAT AT^CGAT
DrdI 3431 GACCAGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1472 GATATC GAT^ATC
FauI 3725 CCCGC CCCGC(4/6)
HaeII 3804 AGCGCC [GA]GCGC^[CT]
Hin1I 6847 GACGTC G[GA]^CG[CT]C
MfeI 438 CAATTG C^AATTG
MunI 438 CAATTG C^AATTG
NciI 7273 CCCGG CC^[GC]GG
NdeI 208 CATATG CA^TATG
NheI 3109 GCTAGC G^CTAGC
NruI 700 TCGCGA TCG^CGA
PflFI 7271 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2623 ACCGGT A^CCGGT
PshAI 1391 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4963 AACGTT AA^CGTT
SalI 2849 GTCGAC G^TCGAC
SfiI 6535 GGCCTCTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 5464 TACGTA TAC^GTA
SphI 6181 GCATGC GCATG^C
SwaI 2556 ATTTAAAT ATTT^AAAT
Tth111I 7271 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2849 GTCGAC GT^[AC][GT]AC
5093 GTATAC
AccII 701 CGCG CG^CG
7383 CGCG
AseI 1121 ATTAAT AT^TAAT
7638 ATTAAT
AsnI 1121 ATTAAT AT^TAAT
7638 ATTAAT
AvaI 53 CTCGGG C^[CT]CG[GA]G
2750 CTCGGG
BalI 4336 TGGCCA TGG^CCA
4623 TGGCCA
BglI 6536 GCCTCTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7131 GCCTGAGGGGC
BlpI 4208 GCTGAGC GC^T[ACGT]AGC
4605 GCTCAGC
Bpu1102I 4208 GCTGAGC GC^T[ACGT]AGC
4605 GCTCAGC
BsmBI 25 GAGACG CGTCTC(1/5)
6845 GAGACG
BsoBI 53 CTCGGG C^[CT]CG[GA]G
2750 CTCGGG
BsrBI 3726 CCGCTC CCGCTC(-3/-3)
5030 GAGCGG
BsrFI 2623 ACCGGT [GA]^CCGG[CT]
5616 GCCGGT
BstEII 2149 GGTAACC G^GT[ACGT]ACC
3481 GGTAACC
BstPI 2149 GGTAACC G^GT[ACGT]ACC
3481 GGTAACC
BstUI 701 CGCG CG^CG
7383 CGCG
CelII 4208 GCTGAGC GC^T[ACGT]AGC
4605 GCTCAGC
Cfr10I 2623 ACCGGT [GA]^CCGG[CT]
5616 GCCGGT
DraI 1178 TTTAAA TTT^AAA
2557 TTTAAA
EcoO65I 2149 GGTAACC G^GT[ACGT]ACC
3481 GGTAACC
EcoRI 926 GAATTC G^AATTC
1975 GAATTC
EcoT22I 300 ATGCAT ATGCA^T
973 ATGCAT
MluNI 4336 TGGCCA TGG^CCA
4623 TGGCCA
MscI 4336 TGGCCA TGG^CCA
4623 TGGCCA
MvnI 701 CGCG CG^CG
7383 CGCG
NsiI 300 ATGCAT ATGCA^T
973 ATGCAT
PshBI 1121 ATTAAT AT^TAAT
7638 ATTAAT
SapI 47 GAAGAGC GCTCTTC(1/4)
5594 GAAGAGC
ScaI 3159 AGTACT AGT^ACT
7107 AGTACT
SexAI 5913 ACCTGGT A^CC[AT]GGT
7199 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaII AvrII BanI
BanII BbsI BbvCI BbvI BciVI
BclI BfaI BglII BlnI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI