##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccI AccIII AgeI Aor51HI
AscI AspI AviII BglI BseAI
BsiEI BsiWI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoT22I FseI FspI
KspI MluI MroI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2184 GGTACC G^GTACC
AhdI 2540 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 784 GGGCCC GGGCC^C
AseI 4653 ATTAAT AT^TAAT
AsnI 4653 ATTAAT AT^TAAT
Asp718I 2184 GGTACC G^GTACC
AspEI 2540 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2730 GGCGCC GGCGC^C
BbrPI 1637 CACGTG CAC^GTG
BbsI 5864 GTCTTC GAAGAC(2/6)
BcgI 413 CGAAGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 265 TGATCA T^GATCA
BglII 5591 AGATCT A^GATCT
BpuAI 5864 GTCTTC GAAGAC(2/6)
BsmI 6815 GAATGC GAATGC(1/-1)
Bsp1407I 4595 TGTACA T^GTACA
BsrFI 2434 GCCGGC [GA]^CCGG[CT]
BsrGI 4595 TGTACA T^GTACA
BstEII 2110 GGTCACC G^GT[ACGT]ACC
BstPI 2110 GGTCACC G^GT[ACGT]ACC
Cfr10I 2434 GCCGGC [GA]^CCGG[CT]
DraIII 1725 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2540 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2110 GGTCACC G^GT[ACGT]ACC
EcoRV 4729 GATATC GAT^ATC
FbaI 265 TGATCA T^GATCA
HpaI 6987 GTTAAC GTT^AAC
KasI 2730 GGCGCC G^GCGCC
KpnI 2184 GGTACC GGTAC^C
MfeI 1747 CAATTG C^AATTG
MunI 1747 CAATTG C^AATTG
NaeI 2434 GCCGGC GCC^GGC
NarI 2730 GGCGCC GG^CGCC
NcoI 2082 CCATGG C^CATGG
NdeI 5430 CATATG CA^TATG
NgoMIV 2434 GCCGGC G^CCGGC
NheI 3750 GCTAGC G^CTAGC
PmaCI 1637 CACGTG CAC^GTG
PmlI 1637 CACGTG CAC^GTG
PshAI 6853 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4653 ATTAAT AT^TAAT
SacI 5528 GAGCTC GAGCT^C
SapI 4385 GCTCTTC GCTCTTC(1/4)
SexAI 1575 ACCTGGT A^CC[AT]GGT
SfoI 2730 GGCGCC GGC^GCC
SmaI 3027 CCCGGG CCC^GGG
SpeI 3303 ACTAGT A^CTAGT
SspBI 4595 TGTACA T^GTACA
SspI 160 AATATT AAT^ATT
XmaCI 3027 CCCGGG C^CCGGG
XmaI 3027 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 599 CGCG CG^CG
2871 CGCG
AclI 1686 AACGTT AA^CGTT
2207 AACGTT
AflII 1313 CTTAAG C^TTAAG
3833 CTTAAG
Alw44I 278 GTGCAC G^TGCAC
1229 GTGCAC
ApaLI 278 GTGCAC G^TGCAC
1229 GTGCAC
BamHI 897 GGATCC G^GATCC
5781 GGATCC
BfrI 1313 CTTAAG C^TTAAG
3833 CTTAAG
BsaAI 1637 CACGTG [CT]AC^GT[GA]
5747 TACGTG
BsgI 459 CTGCAC GTGCAG(16/14)
2264 CTGCAC
BsmBI 2429 GAGACG CGTCTC(1/5)
2867 GAGACG
BsrBI 2350 CCGCTC CCGCTC(-3/-3)
998 GAGCGG
BstUI 599 CGCG CG^CG
2871 CGCG
DsaI 2082 CCATGG C^C[GA][CT]GG
3554 CCGTGG
EcoNI 54 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2378 CCTCAGCGAGG
HaeII 1902 AGCGCC [GA]GCGC^[CT]
2730 GGCGCC
HindIII 635 AAGCTT A^AGCTT
5186 AAGCTT
MvnI 599 CGCG CG^CG
2871 CGCG
Psp1406I 1686 AACGTT AA^CGTT
2207 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII AvrII
BalI BanI BanII BbvCI BbvI
BciVI BcnI BfaI BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI