##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccI AclI AgeI AscI Asp700I
AvrII BbeI BlnI BsiWI BspDI
BspHI BssHII Bst1107I BstZ17I ClaI
CpoI EcoNI FseI HpaI KasI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI SfiI SfoI SgrAI
SnaBI SpeI SphI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 377 TCCGGA T^CCGGA
AflII 4364 CTTAAG C^TTAAG
AhdI 3909 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1317 ATTAAT AT^TAAT
AsnI 1317 ATTAAT AT^TAAT
AspEI 3909 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 535 TGCGCA TGC^GCA
BalI 506 TGGCCA TGG^CCA
BbrPI 2183 CACGTG CAC^GTG
BciVI 2555 GGATAC GTATCC(6/5)
BfaI 585 CTAG C^TAG
BfrI 4364 CTTAAG C^TTAAG
BglII 87 AGATCT A^GATCT
BseAI 377 TCCGGA T^CCGGA
Bsp1407I 4543 TGTACA T^GTACA
BspEI 377 TCCGGA T^CCGGA
BspLU11I 2177 ACATGT A^CATGT
BsrFI 2334 ACCGGC [GA]^CCGG[CT]
BsrGI 4543 TGTACA T^GTACA
BstEII 4322 GGTCACC G^GT[ACGT]ACC
BstPI 4322 GGTCACC G^GT[ACGT]ACC
BstXI 7192 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2334 ACCGGC [GA]^CCGG[CT]
EagI 4943 CGGCCG C^GGCCG
Eam1105I 3909 GACCTCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4943 CGGCCG C^GGCCG
Eco52I 4943 CGGCCG C^GGCCG
EcoO65I 4322 GGTCACC G^GT[ACGT]ACC
EcoRI 99 GAATTC G^AATTC
EcoT22I 2248 ATGCAT ATGCA^T
FspI 535 TGCGCA TGC^GCA
MaeI 585 CTAG C^TAG
MluNI 506 TGGCCA TGG^CCA
MroI 377 TCCGGA T^CCGGA
MscI 506 TGGCCA TGG^CCA
NcoI 2158 CCATGG C^CATGG
NdeI 6347 CATATG CA^TATG
NruI 5255 TCGCGA TCG^CGA
NsiI 2248 ATGCAT ATGCA^T
PmaCI 2183 CACGTG CAC^GTG
PmlI 2183 CACGTG CAC^GTG
PshBI 1317 ATTAAT AT^TAAT
SbfI 7258 CCTGCAGG CCTGCA^GG
SexAI 4525 ACCAGGT A^CC[AT]GGT
Sse8387I 7258 CCTGCAGG CCTGCA^GG
SspBI 4543 TGTACA T^GTACA
StuI 4614 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3573 GACGTC GACGT^C
5928 GACGTC
Acc65I 2862 GGTACC G^GTACC
7471 GGTACC
AocI 4617 CCTGAGG CC^T[ACGT]AGG
6156 CCTCAGG
ApaI 2916 GGGCCC GGGCC^C
5874 GGGCCC
Asp718I 2862 GGTACC G^GTACC
7471 GGTACC
BbvCI 6592 CCTCAGC CCTCAGC(-5/-2)
6965 CCTCAGC
BclI 337 TGATCA T^GATCA
4597 TGATCA
BlpI 5020 GCTTAGC GC^T[ACGT]AGC
6698 GCTCAGC
Bpu1102I 5020 GCTTAGC GC^T[ACGT]AGC
6698 GCTCAGC
BsaBI 324 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2245 GATATGCATC
BsmBI 2642 CGTCTC CGTCTC(1/5)
1969 GAGACG
BstBI 5130 TTCGAA TT^CGAA
7551 TTCGAA
Bsu36I 4617 CCTGAGG CC^T[ACGT]AGG
6156 CCTCAGG
CelII 5020 GCTTAGC GC^T[ACGT]AGC
6698 GCTCAGC
DraI 267 TTTAAA TTT^AAA
7507 TTTAAA
Eco81I 4617 CCTGAGG CC^T[ACGT]AGG
6156 CCTCAGG
FbaI 337 TGATCA T^GATCA
4597 TGATCA
KpnI 2862 GGTACC GGTAC^C
7471 GGTACC
MamI 324 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2245 GATATGCATC
NspI 2177 ACATGT [GA]CATG^[CT]
4668 ACATGC
NspV 5130 TTCGAA TT^CGAA
7551 TTCGAA
PflMI 3465 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5933 CCAGGTCCTGG
PshAI 4146 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5141 GACTCCTGTC
ScaI 5010 AGTACT AGT^ACT
6412 AGTACT
SfuI 5130 TTCGAA TT^CGAA
7551 TTCGAA
Van91I 3465 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5933 CCAGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AspHI AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcgI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII XmaCI XmaI