##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AgeI AhdI Alw44I ApaI
ApaLI AscI AspEI AvrII BbeI
BbrPI BglI BlnI BsiWI BsrBI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
CpoI Eam1105I FseI HincII HindII
HpaI KasI KspI MluI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 7062 GGTACC G^GTACC
AccI 1427 GTAGAC GT^[AC][GT]AC
AccII 5846 CGCG CG^CG
AccIII 980 TCCGGA T^CCGGA
Aor51HI 58 AGCGCT AGC^GCT
Asp718I 7062 GGTACC G^GTACC
AvaI 5193 CTCGGG C^[CT]CG[GA]G
BsaAI 7390 TACGTG [CT]AC^GT[GA]
BseAI 980 TCCGGA T^CCGGA
BsmBI 2116 GAGACG CGTCTC(1/5)
BsoBI 5193 CTCGGG C^[CT]CG[GA]G
BspDI 4467 ATCGAT AT^CGAT
BspEI 980 TCCGGA T^CCGGA
BspLU11I 6696 ACATGT A^CATGT
BspMI 175 ACCTGC ACCTGC(4/8)
BssSI 6074 CTCGTG CACGAG(-5/-1)
BstBI 5154 TTCGAA TT^CGAA
BstUI 5846 CGCG CG^CG
ClaI 4467 ATCGAT AT^CGAT
EagI 226 CGGCCG C^GGCCG
EclXI 226 CGGCCG C^GGCCG
Eco47III 58 AGCGCT AGC^GCT
Eco52I 226 CGGCCG C^GGCCG
EcoRI 655 GAATTC G^AATTC
EcoRV 6983 GATATC GAT^ATC
FauI 4340 GCGGG CCCGC(4/6)
KpnI 7062 GGTACC GGTAC^C
MroI 980 TCCGGA T^CCGGA
MvnI 5846 CGCG CG^CG
NdeI 5337 CATATG CA^TATG
NheI 2351 GCTAGC G^CTAGC
NspV 5154 TTCGAA TT^CGAA
SacI 1009 GAGCTC GAGCT^C
SexAI 297 ACCTGGT A^CC[AT]GGT
SfuI 5154 TTCGAA TT^CGAA
SphI 1870 GCATGC GCATG^C
SspI 6456 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 743 GACGTC GACGT^C
2337 GACGTC
AcyI 743 GACGTC G[GA]^CG[CT]C
2337 GACGTC
AflII 872 CTTAAG C^TTAAG
4999 CTTAAG
AflIII 6696 ACATGT A^C[GA][CT]GT
7391 ACGTGT
Asp700I 1149 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7440 GAATCCTTTC
AspI 520 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2118 GACGGTGTC
BamHI 966 GGATCC G^GATCC
3077 GGATCC
BbvCI 4659 CCTCAGC CCTCAGC(-5/-2)
1624 GCTGAGG
BclI 1909 TGATCA T^GATCA
3840 TGATCA
BfrI 872 CTTAAG C^TTAAG
4999 CTTAAG
BlpI 4037 GCTGAGC GC^T[ACGT]AGC
5981 GCTGAGC
Bpu1102I 4037 GCTGAGC GC^T[ACGT]AGC
5981 GCTGAGC
BsaHI 743 GACGTC G[GA]^CG[CT]C
2337 GACGTC
BsaWI 980 TCCGGA [AT]^CCGG[AT]
7059 TCCGGT
BsiEI 226 CGGCCG CG[GA][CT]^CG
7302 CGGTCG
BstAPI 1738 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4408 GCAGTGTGTGC
BstEII 3355 GGTAACC G^GT[ACGT]ACC
3397 GGTCACC
BstPI 3355 GGTAACC G^GT[ACGT]ACC
3397 GGTCACC
CelII 4037 GCTGAGC GC^T[ACGT]AGC
5981 GCTGAGC
DraIII 2022 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
5368 CACAAGGTG
EcoNI 4141 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6508 CCTCTACAAGG
EcoO65I 3355 GGTAACC G^GT[ACGT]ACC
3397 GGTCACC
FbaI 1909 TGATCA T^GATCA
3840 TGATCA
HaeII 58 AGCGCT [GA]GCGC^[CT]
6463 AGCGCC
Hin1I 743 GACGTC G[GA]^CG[CT]C
2337 GACGTC
NcoI 2268 CCATGG C^CATGG
5348 CCATGG
PflFI 520 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2118 GACGGTGTC
PshAI 3222 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4997 GACTTAAGTC
Tth111I 520 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2118 GACGGTGTC
XbaI 3230 TCTAGA T^CTAGA
7400 TCTAGA
XcmI 811 CCATCTCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3998 CCACCAAACCCTTGG
XmnI 1149 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7440 GAATCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AseI AsnI
AspHI AvaII AviII BalI BanI
BanII BbsI BbvI BcgI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI FspI HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII