##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tsp509I
Tth111I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 921 GTCTAC GT^[AC][GT]AC
AflIII 685 ACGTGT A^C[GA][CT]GT
Alw44I 1132 GTGCAC G^TGCAC
AlwNI 303 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 845 GGGCCC GGGCC^C
ApaLI 1132 GTGCAC G^TGCAC
AspHI 1132 GTGCAC G[AT]GC[AT]^C
BalI 100 TGGCCA TGG^CCA
BamHI 808 GGATCC G^GATCC
BbeI 867 GGCGCC GGCGC^C
BsaAI 684 TACGTG [CT]AC^GT[GA]
BsaI 561 GGTCTC GGTCTC(1/5)
BseRI 666 GAGGAG GAGGAG(10/8)
BsiHKAI 1132 GTGCAC G[AT]GC[AT]^C
Bsp1407I 142 TGTACA T^GTACA
BsrGI 142 TGTACA T^GTACA
BsrI 767 CCAGT ACTGG(1/-1)
BssHII 880 GCGCGC G^CGCGC
EcoT14I 376 CCATGG C^C[AT][AT]GG
HaeII 867 GGCGCC [GA]GCGC^[CT]
KasI 867 GGCGCC G^GCGCC
MaeII 685 ACGT A^CGT
MluNI 100 TGGCCA TGG^CCA
MscI 100 TGGCCA TGG^CCA
NaeI 699 GCCGGC GCC^GGC
NarI 867 GGCGCC GG^CGCC
NcoI 376 CCATGG C^CATGG
NgoMIV 699 GCCGGC G^CCGGC
PpuMI 964 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 227 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 867 GGCGCC GGC^GCC
SmaI 1148 CCCGGG CCC^GGG
SmlI 1218 CTCAAG C^T[CT][GA]AG
SspBI 142 TGTACA T^GTACA
StuI 973 AGGCCT AGG^CCT
StyI 376 CCATGG C^C[AT][AT]GG
TaqI 94 TCGA T^CGA
TspRI 484 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 94 TCGA T^CGA
XcmI 1236 CCAATCCTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1148 CCCGGG C^CCGGG
XmaI 1148 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 965 GGACC G^G[AT]CC
1118 GGTCC
BanII 845 GGGCCC G[GA]GC[CT]^C
979 GAGCCC
BbsI 755 GAAGAC GAAGAC(2/6)
1064 GAAGAC
BfaI 1043 CTAG C^TAG
1224 CTAG
BglI 723 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1003 GCCAGCTGGGC
BpuAI 755 GAAGAC GAAGAC(2/6)
1064 GAAGAC
BsgI 1161 CTGCAC GTGCAG(16/14)
1112 GTGCAG
BsmAI 562 GTCTC GTCTC(1/5)
1180 GTCTC
BsrDI 416 CATTGC GCAATG(2/0)
554 CATTGC
BssSI 146 CACGAG CACGAG(-5/-1)
840 CACGAG
BstF5I 404 GGATG GGATG(2/0)
596 GGATG
FokI 404 GGATG GGATG(9/13)
596 GGATG
HincII 735 GTCAAC GT[CT]^[GA]AC
786 GTCAAC
HindII 735 GTCAAC GT[CT]^[GA]AC
786 GTCAAC
HinfI 574 GACTC G^A[ACGT]TC
942 GAGTC
MaeI 1043 CTAG C^TAG
1224 CTAG
MseI 5 TTAA T^TAA
319 TTAA
NheI 1042 GCTAGC G^CTAGC
1223 GCTAGC
NspI 151 GCATGT [GA]CATG^[CT]
199 GCATGT
PleI 574 GACTC GAGTC(4/5)
942 GAGTC
Tru9I 5 TTAA T^TAA
319 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI BanI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BssKI BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I XhoII