################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AgeI Alw44I ApaLI AscI BbeI BglII BsiWI BspHI BssHII CpoI DraI HpaI KasI KspI MfeI MluI MunI NarI NdeI NotI NruI PacI PaeR7I PinAI PmeI PvuI RcaI RsrII SacII SalI ScaI SfoI SgrAI SmaI SnaBI SpeI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 208 GACGTC GACGT^C Acc65I 11 GGTACC G^GTACC AccI 4850 GTATAC GT^[AC][GT]AC AccII 6205 CGCG CG^CG AccIII 2180 TCCGGA T^CCGGA AclI 2528 AACGTT AA^CGTT AflII 250 CTTAAG C^TTAAG AhdI 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 4480 AGCGCT AGC^GCT Asp700I 4171 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 11 GGTACC G^GTACC AspEI 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2444 TGCGCA TGC^GCA BcgI 3267 GCATGGTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 250 CTTAAG C^TTAAG BseAI 2180 TCCGGA T^CCGGA BsiEI 2107 CGGCCG CG[GA][CT]^CG BsmBI 5848 GAGACG CGTCTC(1/5) BsmI 4021 GAATGC GAATGC(1/-1) BspEI 2180 TCCGGA T^CCGGA Bst1107I 4850 GTATAC GTA^TAC BstUI 6205 CGCG CG^CG BstZ17I 4850 GTATAC GTA^TAC DrdI 1545 GACGGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2107 CGGCCG C^GGCCG Eam1105I 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2107 CGGCCG C^GGCCG Eco47III 4480 AGCGCT AGC^GCT Eco52I 2107 CGGCCG C^GGCCG EcoT22I 6344 ATGCAT ATGCA^T FspI 2444 TGCGCA TGC^GCA HincII 516 GTCAAC GT[CT]^[GA]AC HindII 516 GTCAAC GT[CT]^[GA]AC HindIII 1903 AAGCTT A^AGCTT KpnI 11 GGTACC GGTAC^C MroI 2180 TCCGGA T^CCGGA MvnI 6205 CGCG CG^CG NheI 3345 GCTAGC G^CTAGC NsiI 6344 ATGCAT ATGCA^T Psp1406I 2528 AACGTT AA^CGTT SexAI 1922 ACCTGGT A^CC[AT]GGT SfiI 825 GGCCCGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3705 GCATGC GCATG^C SspI 633 AATATT AAT^ATT XmnI 4171 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AseI 5972 ATTAAT AT^TAAT 6118 ATTAAT AsnI 5972 ATTAAT AT^TAAT 6118 ATTAAT BbrPI 1987 CACGTG CAC^GTG 4504 CACGTG BciVI 979 GGATAC GTATCC(6/5) 488 GTATCC BclI 185 TGATCA T^GATCA 1835 TGATCA BsaAI 1987 CACGTG [CT]AC^GT[GA] 4504 CACGTG BsaWI 1088 ACCGGA [AT]^CCGG[AT] 2180 TCCGGA Bsp1407I 1917 TGTACA T^GTACA 6084 TGTACA BspDI 1840 ATCGAT AT^CGAT 5421 ATCGAT BsrGI 1917 TGTACA T^GTACA 6084 TGTACA BssSI 189 CACGAG CACGAG(-5/-1) 6478 CACGAG BstBI 2700 TTCGAA TT^CGAA 5717 TTCGAA BstEII 2127 GGTCACC G^GT[ACGT]ACC 4659 GGTAACC BstPI 2127 GGTCACC G^GT[ACGT]ACC 4659 GGTAACC ClaI 1840 ATCGAT AT^CGAT 5421 ATCGAT EcoO65I 2127 GGTCACC G^GT[ACGT]ACC 4659 GGTAACC EcoRV 3860 GATATC GAT^ATC 4389 GATATC FbaI 185 TGATCA T^GATCA 1835 TGATCA FseI 1300 GGCCGGCC GGCCGG^CC 2104 GGCCGGCC NspV 2700 TTCGAA TT^CGAA 5717 TTCGAA PmaCI 1987 CACGTG CAC^GTG 4504 CACGTG PmlI 1987 CACGTG CAC^GTG 4504 CACGTG PshBI 5972 ATTAAT AT^TAAT 6118 ATTAAT SacI 3250 GAGCTC GAGCT^C 4093 GAGCTC SapI 1227 GAAGAGC GCTCTTC(1/4) 2145 GCTCTTC SbfI 1599 CCTGCAGG CCTGCA^GG 5594 CCTGCAGG SfuI 2700 TTCGAA TT^CGAA 5717 TTCGAA Sse8387I 1599 CCTGCAGG CCTGCA^GG 5594 CCTGCAGG SspBI 1917 TGTACA T^GTACA 6084 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII AvrII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII