##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AgeI Alw44I ApaLI AscI BbeI
BglII BsiWI BspHI BssHII CpoI
DraI HpaI KasI KspI MfeI
MluI MunI NarI NdeI NotI
NruI PacI PaeR7I PinAI PmeI
PvuI RcaI RsrII SacII SalI
ScaI SfoI SgrAI SmaI SnaBI
SpeI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 208 GACGTC GACGT^C
Acc65I 11 GGTACC G^GTACC
AccI 4850 GTATAC GT^[AC][GT]AC
AccII 6205 CGCG CG^CG
AccIII 2180 TCCGGA T^CCGGA
AclI 2528 AACGTT AA^CGTT
AflII 250 CTTAAG C^TTAAG
AhdI 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4480 AGCGCT AGC^GCT
Asp700I 4171 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 11 GGTACC G^GTACC
AspEI 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2444 TGCGCA TGC^GCA
BcgI 3267 GCATGGTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 250 CTTAAG C^TTAAG
BseAI 2180 TCCGGA T^CCGGA
BsiEI 2107 CGGCCG CG[GA][CT]^CG
BsmBI 5848 GAGACG CGTCTC(1/5)
BsmI 4021 GAATGC GAATGC(1/-1)
BspEI 2180 TCCGGA T^CCGGA
Bst1107I 4850 GTATAC GTA^TAC
BstUI 6205 CGCG CG^CG
BstZ17I 4850 GTATAC GTA^TAC
DrdI 1545 GACGGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2107 CGGCCG C^GGCCG
Eam1105I 4426 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2107 CGGCCG C^GGCCG
Eco47III 4480 AGCGCT AGC^GCT
Eco52I 2107 CGGCCG C^GGCCG
EcoT22I 6344 ATGCAT ATGCA^T
FspI 2444 TGCGCA TGC^GCA
HincII 516 GTCAAC GT[CT]^[GA]AC
HindII 516 GTCAAC GT[CT]^[GA]AC
HindIII 1903 AAGCTT A^AGCTT
KpnI 11 GGTACC GGTAC^C
MroI 2180 TCCGGA T^CCGGA
MvnI 6205 CGCG CG^CG
NheI 3345 GCTAGC G^CTAGC
NsiI 6344 ATGCAT ATGCA^T
Psp1406I 2528 AACGTT AA^CGTT
SexAI 1922 ACCTGGT A^CC[AT]GGT
SfiI 825 GGCCCGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3705 GCATGC GCATG^C
SspI 633 AATATT AAT^ATT
XmnI 4171 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AseI 5972 ATTAAT AT^TAAT
6118 ATTAAT
AsnI 5972 ATTAAT AT^TAAT
6118 ATTAAT
BbrPI 1987 CACGTG CAC^GTG
4504 CACGTG
BciVI 979 GGATAC GTATCC(6/5)
488 GTATCC
BclI 185 TGATCA T^GATCA
1835 TGATCA
BsaAI 1987 CACGTG [CT]AC^GT[GA]
4504 CACGTG
BsaWI 1088 ACCGGA [AT]^CCGG[AT]
2180 TCCGGA
Bsp1407I 1917 TGTACA T^GTACA
6084 TGTACA
BspDI 1840 ATCGAT AT^CGAT
5421 ATCGAT
BsrGI 1917 TGTACA T^GTACA
6084 TGTACA
BssSI 189 CACGAG CACGAG(-5/-1)
6478 CACGAG
BstBI 2700 TTCGAA TT^CGAA
5717 TTCGAA
BstEII 2127 GGTCACC G^GT[ACGT]ACC
4659 GGTAACC
BstPI 2127 GGTCACC G^GT[ACGT]ACC
4659 GGTAACC
ClaI 1840 ATCGAT AT^CGAT
5421 ATCGAT
EcoO65I 2127 GGTCACC G^GT[ACGT]ACC
4659 GGTAACC
EcoRV 3860 GATATC GAT^ATC
4389 GATATC
FbaI 185 TGATCA T^GATCA
1835 TGATCA
FseI 1300 GGCCGGCC GGCCGG^CC
2104 GGCCGGCC
NspV 2700 TTCGAA TT^CGAA
5717 TTCGAA
PmaCI 1987 CACGTG CAC^GTG
4504 CACGTG
PmlI 1987 CACGTG CAC^GTG
4504 CACGTG
PshBI 5972 ATTAAT AT^TAAT
6118 ATTAAT
SacI 3250 GAGCTC GAGCT^C
4093 GAGCTC
SapI 1227 GAAGAGC GCTCTTC(1/4)
2145 GCTCTTC
SbfI 1599 CCTGCAGG CCTGCA^GG
5594 CCTGCAGG
SfuI 2700 TTCGAA TT^CGAA
5717 TTCGAA
Sse8387I 1599 CCTGCAGG CCTGCA^GG
5594 CCTGCAGG
SspBI 1917 TGTACA T^GTACA
6084 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII