##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AviII BbrPI
BbvCI BcgI BciVI BsiEI BsiWI
Bsp1407I BspDI BsrFI BsrGI BssHII
BstBI BstEII BstPI BstXI Cfr10I
ClaI CpoI EagI EclXI Eco52I
EcoO65I FseI FspI HincII HindII
HpaI KpnI KspI MluI NaeI
NgoMIV NheI NotI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AspI 1325 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 291 GGCGCC GGCGC^C
BglI 5562 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1275 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1275 GCTAAGC GC^T[ACGT]AGC
BsmBI 149 CGTCTC CGTCTC(1/5)
BssSI 3842 CTCGTG CACGAG(-5/-1)
Bst1107I 6615 GTATAC GTA^TAC
BstZ17I 6615 GTATAC GTA^TAC
CelII 1275 GCTAAGC GC^T[ACGT]AGC
DrdI 536 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4569 GAATTC G^AATTC
EcoT22I 4521 ATGCAT ATGCA^T
HindIII 5266 AAGCTT A^AGCTT
KasI 291 GGCGCC G^GCGCC
MfeI 125 CAATTG C^AATTG
MunI 125 CAATTG C^AATTG
NarI 291 GGCGCC GG^CGCC
NruI 7374 TCGCGA TCG^CGA
NsiI 4521 ATGCAT ATGCA^T
PflFI 1325 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 3652 CAGCTG CAG^CTG
SexAI 2688 ACCAGGT A^CC[AT]GGT
SfoI 291 GGCGCC GGC^GCC
SmaI 1075 CCCGGG CCC^GGG
SpeI 7145 ACTAGT A^CTAGT
SphI 5282 GCATGC GCATG^C
Tth111I 1325 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 7100 TCTAGA T^CTAGA
XmaCI 1075 CCCGGG C^CCGGG
XmaI 1075 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 6615 GTATAC GT^[AC][GT]AC
7571 GTAGAC
AcyI 291 GGCGCC G[GA]^CG[CT]C
1250 GACGCC
AflII 1095 CTTAAG C^TTAAG
5204 CTTAAG
AhdI 1864 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5137 GACCAGCAGTC
Alw44I 4434 GTGCAC G^TGCAC
6587 GTGCAC
ApaLI 4434 GTGCAC G^TGCAC
6587 GTGCAC
AspEI 1864 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5137 GACCAGCAGTC
AvaI 1075 CCCGGG C^[CT]CG[GA]G
2086 CTCGGG
BamHI 139 GGATCC G^GATCC
242 GGATCC
BclI 3994 TGATCA T^GATCA
5879 TGATCA
BfrI 1095 CTTAAG C^TTAAG
5204 CTTAAG
BglII 2035 AGATCT A^GATCT
4010 AGATCT
BsaAI 5491 CACGTA [CT]AC^GT[GA]
7008 TACGTG
BsaHI 291 GGCGCC G[GA]^CG[CT]C
1250 GACGCC
BsmI 1613 GAATGC GAATGC(1/-1)
5209 GAATGC
BsoBI 1075 CCCGGG C^[CT]CG[GA]G
2086 CTCGGG
BspHI 1729 TCATGA T^CATGA
5077 TCATGA
BsrBI 30 CCGCTC CCGCTC(-3/-3)
735 GAGCGG
Eam1105I 1864 GACACTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5137 GACCAGCAGTC
EcoNI 4822 CCTCACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5823 CCTGAGTTAGG
EcoRV 3049 GATATC GAT^ATC
7257 GATATC
FbaI 3994 TGATCA T^GATCA
5879 TGATCA
Hin1I 291 GGCGCC G[GA]^CG[CT]C
1250 GACGCC
MspA1I 363 CCGCTG C[AC]G^C[GT]G
3652 CAGCTG
PflMI 2430 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5664 CCACACTGTGG
RcaI 1729 TCATGA T^CATGA
5077 TCATGA
SapI 2858 GAAGAGC GCTCTTC(1/4)
3008 GAAGAGC
StuI 2313 AGGCCT AGG^CCT
4810 AGGCCT
Van91I 2430 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5664 CCACACTGTGG
XcmI 1246 CCATGACGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
7062 CCAGTAATGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI Asp700I AspHI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspEI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco47III
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI