################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbrPI BfrI BglI BlnI BlpI Bpu1102I BsaHI BseAI BsiEI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 809 ACGCGT A^C[GA][CT]GT Alw44I 1551 GTGCAC G^TGCAC AlwNI 1173 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1551 GTGCAC G^TGCAC AseI 1873 ATTAAT AT^TAAT AsnI 1873 ATTAAT AT^TAAT BanII 677 GGGCTC G[GA]GC[CT]^C BbsI 1905 GAAGAC GAAGAC(2/6) BcgI 2453 GCATGCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1673 GGATAC GTATCC(6/5) BclI 2050 TGATCA T^GATCA BglII 1074 AGATCT A^GATCT BpmI 245 CTCCAG CTGGAG(16/14) BpuAI 1905 GAAGAC GAAGAC(2/6) BsaAI 1252 CACGTA [CT]AC^GT[GA] BsaBI 353 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1408 GAGACC GGTCTC(1/5) BsaWI 389 TCCGGT [AT]^CCGG[AT] BsiWI 1732 CGTACG C^GTACG BsmBI 250 GAGACG CGTCTC(1/5) BstAPI 581 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CfoI 1666 GCGC GCG^C DraI 2433 TTTAAA TTT^AAA DraII 685 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 2018 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 685 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 1787 CCCGC CCCGC(4/6) FbaI 2050 TGATCA T^GATCA HaeIII 468 GGCC GG^CC HhaI 1666 GCGC GCG^C HinP1I 1666 GCGC G^CGC MaeII 1253 ACGT A^CGT MamI 353 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 809 ACGCGT A^CGCGT PacI 2282 TTAATTAA TTAAT^TAA PflMI 1172 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 2014 GACTC GAGTC(4/5) PpuMI 685 AGGACCT [GA]G^G[AT]CC[CT] PshBI 1873 ATTAAT AT^TAAT SmaI 1582 CCCGGG CCC^GGG SspI 55 AATATT AAT^ATT Van91I 1172 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1582 CCCGGG C^CCGGG XmaI 1582 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 810 CGCG CG^CG 1665 CGCG AcsI 1201 AAATTT [GA]^AATT[CT] 1836 GAATTT ApoI 1201 AAATTT [GA]^AATT[CT] 1836 GAATTT AvaI 37 CCCGAG C^[CT]CG[GA]G 1582 CCCGGG BamHI 560 GGATCC G^GATCC 1102 GGATCC BbvCI 449 CCTCAGC CCTCAGC(-5/-2) 905 CCTCAGC BsgI 1466 CTGCAC GTGCAG(16/14) 714 GTGCAG BsoBI 37 CCCGAG C^[CT]CG[GA]G 1582 CCCGGG BstUI 810 CGCG CG^CG 1665 CGCG EcoNI 659 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2162 CCTATAACAGG EcoT22I 1232 ATGCAT ATGCA^T 2307 ATGCAT MvnI 810 CGCG CG^CG 1665 CGCG NcoI 1500 CCATGG C^CATGG 1989 CCATGG NsiI 1232 ATGCAT ATGCA^T 2307 ATGCAT PstI 1164 CTGCAG CTGCA^G 1569 CTGCAG SexAI 658 ACCTGGT A^CC[AT]GGT 1512 ACCAGGT SmlI 14 CTCAAG C^T[CT][GA]AG 950 CTCAAG SphI 2065 GCATGC GCATG^C 2453 GCATGC TaqI 503 TCGA T^CGA 2038 TCGA TfiI 788 GAATC G^A[AT]TC 1113 GAATC TthHB8I 503 TCGA T^CGA 2038 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII