##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BfrI BglI
BlnI BlpI Bpu1102I BsaHI BseAI
BsiEI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluNI MroI MscI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 809 ACGCGT A^C[GA][CT]GT
Alw44I 1551 GTGCAC G^TGCAC
AlwNI 1173 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1551 GTGCAC G^TGCAC
AseI 1873 ATTAAT AT^TAAT
AsnI 1873 ATTAAT AT^TAAT
BanII 677 GGGCTC G[GA]GC[CT]^C
BbsI 1905 GAAGAC GAAGAC(2/6)
BcgI 2453 GCATGCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1673 GGATAC GTATCC(6/5)
BclI 2050 TGATCA T^GATCA
BglII 1074 AGATCT A^GATCT
BpmI 245 CTCCAG CTGGAG(16/14)
BpuAI 1905 GAAGAC GAAGAC(2/6)
BsaAI 1252 CACGTA [CT]AC^GT[GA]
BsaBI 353 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1408 GAGACC GGTCTC(1/5)
BsaWI 389 TCCGGT [AT]^CCGG[AT]
BsiWI 1732 CGTACG C^GTACG
BsmBI 250 GAGACG CGTCTC(1/5)
BstAPI 581 GCAGTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1666 GCGC GCG^C
DraI 2433 TTTAAA TTT^AAA
DraII 685 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 2018 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 685 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 1787 CCCGC CCCGC(4/6)
FbaI 2050 TGATCA T^GATCA
HaeIII 468 GGCC GG^CC
HhaI 1666 GCGC GCG^C
HinP1I 1666 GCGC G^CGC
MaeII 1253 ACGT A^CGT
MamI 353 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 809 ACGCGT A^CGCGT
PacI 2282 TTAATTAA TTAAT^TAA
PflMI 1172 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2014 GACTC GAGTC(4/5)
PpuMI 685 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1873 ATTAAT AT^TAAT
SmaI 1582 CCCGGG CCC^GGG
SspI 55 AATATT AAT^ATT
Van91I 1172 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1582 CCCGGG C^CCGGG
XmaI 1582 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 810 CGCG CG^CG
1665 CGCG
AcsI 1201 AAATTT [GA]^AATT[CT]
1836 GAATTT
ApoI 1201 AAATTT [GA]^AATT[CT]
1836 GAATTT
AvaI 37 CCCGAG C^[CT]CG[GA]G
1582 CCCGGG
BamHI 560 GGATCC G^GATCC
1102 GGATCC
BbvCI 449 CCTCAGC CCTCAGC(-5/-2)
905 CCTCAGC
BsgI 1466 CTGCAC GTGCAG(16/14)
714 GTGCAG
BsoBI 37 CCCGAG C^[CT]CG[GA]G
1582 CCCGGG
BstUI 810 CGCG CG^CG
1665 CGCG
EcoNI 659 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2162 CCTATAACAGG
EcoT22I 1232 ATGCAT ATGCA^T
2307 ATGCAT
MvnI 810 CGCG CG^CG
1665 CGCG
NcoI 1500 CCATGG C^CATGG
1989 CCATGG
NsiI 1232 ATGCAT ATGCA^T
2307 ATGCAT
PstI 1164 CTGCAG CTGCA^G
1569 CTGCAG
SexAI 658 ACCTGGT A^CC[AT]GGT
1512 ACCAGGT
SmlI 14 CTCAAG C^T[CT][GA]AG
950 CTCAAG
SphI 2065 GCATGC GCATG^C
2453 GCATGC
TaqI 503 TCGA T^CGA
2038 TCGA
TfiI 788 GAATC G^A[AT]TC
1113 GAATC
TthHB8I 503 TCGA T^CGA
2038 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII