##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbrPI BcgI BciVI
BclI BfrI BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 502 CGCG CG^CG
AocI 1961 CCTGAGG CC^T[ACGT]AGG
Asp700I 1979 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2217 TGGCCA TGG^CCA
BamHI 1795 GGATCC G^GATCC
BbvCI 2123 CCTCAGC CCTCAGC(-5/-2)
BglI 2210 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1132 AGATCT A^GATCT
BlpI 1425 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1425 GCTCAGC GC^T[ACGT]AGC
BsaAI 550 CACGTA [CT]AC^GT[GA]
BsaWI 1451 ACCGGA [AT]^CCGG[AT]
BsiEI 2308 CGACCG CG[GA][CT]^CG
BsmI 1172 GAATGC GAATGC(1/-1)
BsrBI 146 CCGCTC CCGCTC(-3/-3)
BsrI 1445 ACTGG ACTGG(1/-1)
BstEII 2152 GGTGACC G^GT[ACGT]ACC
BstPI 2152 GGTGACC G^GT[ACGT]ACC
BstUI 502 CGCG CG^CG
Bsu36I 1961 CCTGAGG CC^T[ACGT]AGG
CelII 1425 GCTCAGC GC^T[ACGT]AGC
DraI 1998 TTTAAA TTT^AAA
Eco81I 1961 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2152 GGTGACC G^GT[ACGT]ACC
HincII 1067 GTTGAC GT[CT]^[GA]AC
HindII 1067 GTTGAC GT[CT]^[GA]AC
KspI 501 CCGCGG CCGC^GG
MluNI 2217 TGGCCA TGG^CCA
MscI 2217 TGGCCA TGG^CCA
MslI 1753 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 502 CGCG CG^CG
NaeI 1421 GCCGGC GCC^GGC
NcoI 29 CCATGG C^CATGG
NgoMIV 1421 GCCGGC G^CCGGC
PaeR7I 1136 CTCGAG C^TCGAG
PflMI 286 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1716 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 501 CCGCGG CCGC^GG
SapI 1193 GAAGAGC GCTCTTC(1/4)
SgrAI 445 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2100 CCCGGG CCC^GGG
Van91I 286 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1136 CTCGAG C^TCGAG
XmaCI 2100 CCCGGG C^CCGGG
XmaI 2100 CCCGGG C^CCGGG
XmnI 1979 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 265 GAATTT [GA]^AATT[CT]
2002 AAATTT
AfaI 1112 GTAC GT^AC
1530 GTAC
Alw44I 403 GTGCAC G^TGCAC
442 GTGCAC
ApaI 42 GGGCCC GGGCC^C
300 GGGCCC
ApaLI 403 GTGCAC G^TGCAC
442 GTGCAC
ApoI 265 GAATTT [GA]^AATT[CT]
2002 AAATTT
AspI 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2342 GACGGAGTC
AvaI 1136 CTCGAG C^[CT]CG[GA]G
2100 CCCGGG
BanI 506 GGCGCC G^G[CT][GA]CC
1512 GGCGCC
BbeI 506 GGCGCC GGCGC^C
1512 GGCGCC
BsmBI 1494 CGTCTC CGTCTC(1/5)
2340 GAGACG
BsoBI 1136 CTCGAG C^[CT]CG[GA]G
2100 CCCGGG
BstXI 1295 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2271 CCAAAGTGCTGG
EaeI 1577 TGGCCG [CT]^GGCC[GA]
2217 TGGCCA
EcoNI 1910 CCTAACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1932 CCTGGGGGAGG
FauI 1460 CCCGC CCCGC(4/6)
503 GCGGG
HgaI 1972 GACGC GACGC(5/10)
1493 GCGTC
KasI 506 GGCGCC G^GCGCC
1512 GGCGCC
NarI 506 GGCGCC GG^CGCC
1512 GGCGCC
PflFI 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2342 GACGGAGTC
PpuMI 69 GGGACCC [GA]G^G[AT]CC[CT]
768 GGGTCCT
RsaI 1112 GTAC GT^AC
1530 GTAC
SacI 1196 GAGCTC GAGCT^C
1355 GAGCTC
SfaNI 257 GATGC GCATC(5/9)
1582 GATGC
SfoI 506 GGCGCC GGC^GCC
1512 GGCGCC
StuI 1765 AGGCCT AGG^CCT
1907 AGGCCT
Tth111I 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2342 GACGGAGTC
XcmI 29 CCATGGCTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
549 CCACGTAACTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrFI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII