################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BcgI BciVI BclI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 502 CGCG CG^CG AocI 1961 CCTGAGG CC^T[ACGT]AGG Asp700I 1979 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2217 TGGCCA TGG^CCA BamHI 1795 GGATCC G^GATCC BbvCI 2123 CCTCAGC CCTCAGC(-5/-2) BglI 2210 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1132 AGATCT A^GATCT BlpI 1425 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1425 GCTCAGC GC^T[ACGT]AGC BsaAI 550 CACGTA [CT]AC^GT[GA] BsaWI 1451 ACCGGA [AT]^CCGG[AT] BsiEI 2308 CGACCG CG[GA][CT]^CG BsmI 1172 GAATGC GAATGC(1/-1) BsrBI 146 CCGCTC CCGCTC(-3/-3) BsrI 1445 ACTGG ACTGG(1/-1) BstEII 2152 GGTGACC G^GT[ACGT]ACC BstPI 2152 GGTGACC G^GT[ACGT]ACC BstUI 502 CGCG CG^CG Bsu36I 1961 CCTGAGG CC^T[ACGT]AGG CelII 1425 GCTCAGC GC^T[ACGT]AGC DraI 1998 TTTAAA TTT^AAA Eco81I 1961 CCTGAGG CC^T[ACGT]AGG EcoO65I 2152 GGTGACC G^GT[ACGT]ACC HincII 1067 GTTGAC GT[CT]^[GA]AC HindII 1067 GTTGAC GT[CT]^[GA]AC KspI 501 CCGCGG CCGC^GG MluNI 2217 TGGCCA TGG^CCA MscI 2217 TGGCCA TGG^CCA MslI 1753 CACATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 502 CGCG CG^CG NaeI 1421 GCCGGC GCC^GGC NcoI 29 CCATGG C^CATGG NgoMIV 1421 GCCGGC G^CCGGC PaeR7I 1136 CTCGAG C^TCGAG PflMI 286 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1716 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 501 CCGCGG CCGC^GG SapI 1193 GAAGAGC GCTCTTC(1/4) SgrAI 445 CACCGGCG C[GA]^CCGG[CT]G SmaI 2100 CCCGGG CCC^GGG Van91I 286 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1136 CTCGAG C^TCGAG XmaCI 2100 CCCGGG C^CCGGG XmaI 2100 CCCGGG C^CCGGG XmnI 1979 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 265 GAATTT [GA]^AATT[CT] 2002 AAATTT AfaI 1112 GTAC GT^AC 1530 GTAC Alw44I 403 GTGCAC G^TGCAC 442 GTGCAC ApaI 42 GGGCCC GGGCC^C 300 GGGCCC ApaLI 403 GTGCAC G^TGCAC 442 GTGCAC ApoI 265 GAATTT [GA]^AATT[CT] 2002 AAATTT AspI 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2342 GACGGAGTC AvaI 1136 CTCGAG C^[CT]CG[GA]G 2100 CCCGGG BanI 506 GGCGCC G^G[CT][GA]CC 1512 GGCGCC BbeI 506 GGCGCC GGCGC^C 1512 GGCGCC BsmBI 1494 CGTCTC CGTCTC(1/5) 2340 GAGACG BsoBI 1136 CTCGAG C^[CT]CG[GA]G 2100 CCCGGG BstXI 1295 CCAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2271 CCAAAGTGCTGG EaeI 1577 TGGCCG [CT]^GGCC[GA] 2217 TGGCCA EcoNI 1910 CCTAACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1932 CCTGGGGGAGG FauI 1460 CCCGC CCCGC(4/6) 503 GCGGG HgaI 1972 GACGC GACGC(5/10) 1493 GCGTC KasI 506 GGCGCC G^GCGCC 1512 GGCGCC NarI 506 GGCGCC GG^CGCC 1512 GGCGCC PflFI 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2342 GACGGAGTC PpuMI 69 GGGACCC [GA]G^G[AT]CC[CT] 768 GGGTCCT RsaI 1112 GTAC GT^AC 1530 GTAC SacI 1196 GAGCTC GAGCT^C 1355 GAGCTC SfaNI 257 GATGC GCATC(5/9) 1582 GATGC SfoI 506 GGCGCC GGC^GCC 1512 GGCGCC StuI 1765 AGGCCT AGG^CCT 1907 AGGCCT Tth111I 1840 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2342 GACGGAGTC XcmI 29 CCATGGCTGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 549 CCACGTAACTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcyI AluI AlwI AlwNI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrFI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII