################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BclI BlnI BsaWI BseAI BsiWI BspDI BspEI BssHII BstBI ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I FbaI FseI HgaI HpaI KpnI KspI MluI MroI NheI NotI NruI NspV PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 5261 CGCG CG^CG AcyI 616 GGCGCC G[GA]^CG[CT]C Alw44I 5142 GTGCAC G^TGCAC Aor51HI 2291 AGCGCT AGC^GCT ApaLI 5142 GTGCAC G^TGCAC AspI 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4018 TGCGCA TGC^GCA BbeI 616 GGCGCC GGCGC^C BbrPI 3167 CACGTG CAC^GTG BcgI 3062 CGAAAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4919 GGATAC GTATCC(6/5) BglI 4608 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5509 AGATCT A^GATCT BlpI 447 GCTGAGC GC^T[ACGT]AGC Bpu1102I 447 GCTGAGC GC^T[ACGT]AGC BsaAI 3167 CACGTG [CT]AC^GT[GA] BsaHI 616 GGCGCC G[GA]^CG[CT]C BsiEI 2534 CGACCG CG[GA][CT]^CG BsmBI 4212 GAGACG CGTCTC(1/5) BsrBI 2369 CCGCTC CCGCTC(-3/-3) BssSI 3152 CACGAG CACGAG(-5/-1) BstEII 3230 GGTTACC G^GT[ACGT]ACC BstPI 3230 GGTTACC G^GT[ACGT]ACC BstUI 5261 CGCG CG^CG CelII 447 GCTGAGC GC^T[ACGT]AGC Eco47III 2291 AGCGCT AGC^GCT EcoO65I 3230 GGTTACC G^GT[ACGT]ACC EcoRV 19 GATATC GAT^ATC FspI 4018 TGCGCA TGC^GCA Hin1I 616 GGCGCC G[GA]^CG[CT]C KasI 616 GGCGCC G^GCGCC MvnI 5261 CGCG CG^CG NaeI 2488 GCCGGC GCC^GGC NarI 616 GGCGCC GG^CGCC NgoMIV 2488 GCCGGC G^CCGGC PacI 6418 TTAATTAA TTAAT^TAA PaeR7I 1275 CTCGAG C^TCGAG PflFI 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3167 CACGTG CAC^GTG PmlI 3167 CACGTG CAC^GTG PpuMI 4147 AGGACCT [GA]G^G[AT]CC[CT] SacI 555 GAGCTC GAGCT^C ScaI 53 AGTACT AGT^ACT SfoI 616 GGCGCC GGC^GCC SphI 6487 GCATGC GCATG^C Tth111I 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 5959 TCTAGA T^CTAGA XhoI 1275 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 429 GTATAC GT^[AC][GT]AC 4673 GTATAC AflII 2750 CTTAAG C^TTAAG 6608 CTTAAG BamHI 1305 GGATCC G^GATCC 4779 GGATCC BbvCI 3009 CCTCAGC CCTCAGC(-5/-2) 1206 GCTGAGG BfrI 2750 CTTAAG C^TTAAG 6608 CTTAAG BsaBI 2881 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3828 GATCTGGATC BsaI 1371 GAGACC GGTCTC(1/5) 1446 GAGACC BsmI 878 GAATGC GAATGC(1/-1) 4434 GAATGC Bsp1407I 820 TGTACA T^GTACA 6208 TGTACA BspMI 637 ACCTGC ACCTGC(4/8) 2677 ACCTGC BsrFI 1594 ACCGGC [GA]^CCGG[CT] 2488 GCCGGC BsrGI 820 TGTACA T^GTACA 6208 TGTACA Bst1107I 429 GTATAC GTA^TAC 4673 GTATAC BstZ17I 429 GTATAC GTA^TAC 4673 GTATAC Cfr10I 1594 ACCGGC [GA]^CCGG[CT] 2488 GCCGGC DraI 2070 TTTAAA TTT^AAA 6726 TTTAAA EcoRI 2575 GAATTC G^AATTC 3605 GAATTC EcoT22I 2962 ATGCAT ATGCA^T 6489 ATGCAT HincII 1431 GTTGAC GT[CT]^[GA]AC 5014 GTCAAC HindII 1431 GTTGAC GT[CT]^[GA]AC 5014 GTCAAC MamI 2881 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3828 GATCTGGATC NsiI 2962 ATGCAT ATGCA^T 6489 ATGCAT SpeI 3510 ACTAGT A^CTAGT 5619 ACTAGT SspBI 820 TGTACA T^GTACA 6208 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI