##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BclI BlnI BsaWI
BseAI BsiWI BspDI BspEI BssHII
BstBI ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I FbaI FseI
HgaI HpaI KpnI KspI MluI
MroI NheI NotI NruI NspV
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 5261 CGCG CG^CG
AcyI 616 GGCGCC G[GA]^CG[CT]C
Alw44I 5142 GTGCAC G^TGCAC
Aor51HI 2291 AGCGCT AGC^GCT
ApaLI 5142 GTGCAC G^TGCAC
AspI 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4018 TGCGCA TGC^GCA
BbeI 616 GGCGCC GGCGC^C
BbrPI 3167 CACGTG CAC^GTG
BcgI 3062 CGAAAGGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4919 GGATAC GTATCC(6/5)
BglI 4608 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 5509 AGATCT A^GATCT
BlpI 447 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 447 GCTGAGC GC^T[ACGT]AGC
BsaAI 3167 CACGTG [CT]AC^GT[GA]
BsaHI 616 GGCGCC G[GA]^CG[CT]C
BsiEI 2534 CGACCG CG[GA][CT]^CG
BsmBI 4212 GAGACG CGTCTC(1/5)
BsrBI 2369 CCGCTC CCGCTC(-3/-3)
BssSI 3152 CACGAG CACGAG(-5/-1)
BstEII 3230 GGTTACC G^GT[ACGT]ACC
BstPI 3230 GGTTACC G^GT[ACGT]ACC
BstUI 5261 CGCG CG^CG
CelII 447 GCTGAGC GC^T[ACGT]AGC
Eco47III 2291 AGCGCT AGC^GCT
EcoO65I 3230 GGTTACC G^GT[ACGT]ACC
EcoRV 19 GATATC GAT^ATC
FspI 4018 TGCGCA TGC^GCA
Hin1I 616 GGCGCC G[GA]^CG[CT]C
KasI 616 GGCGCC G^GCGCC
MvnI 5261 CGCG CG^CG
NaeI 2488 GCCGGC GCC^GGC
NarI 616 GGCGCC GG^CGCC
NgoMIV 2488 GCCGGC G^CCGGC
PacI 6418 TTAATTAA TTAAT^TAA
PaeR7I 1275 CTCGAG C^TCGAG
PflFI 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3167 CACGTG CAC^GTG
PmlI 3167 CACGTG CAC^GTG
PpuMI 4147 AGGACCT [GA]G^G[AT]CC[CT]
SacI 555 GAGCTC GAGCT^C
ScaI 53 AGTACT AGT^ACT
SfoI 616 GGCGCC GGC^GCC
SphI 6487 GCATGC GCATG^C
Tth111I 717 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5959 TCTAGA T^CTAGA
XhoI 1275 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 429 GTATAC GT^[AC][GT]AC
4673 GTATAC
AflII 2750 CTTAAG C^TTAAG
6608 CTTAAG
BamHI 1305 GGATCC G^GATCC
4779 GGATCC
BbvCI 3009 CCTCAGC CCTCAGC(-5/-2)
1206 GCTGAGG
BfrI 2750 CTTAAG C^TTAAG
6608 CTTAAG
BsaBI 2881 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3828 GATCTGGATC
BsaI 1371 GAGACC GGTCTC(1/5)
1446 GAGACC
BsmI 878 GAATGC GAATGC(1/-1)
4434 GAATGC
Bsp1407I 820 TGTACA T^GTACA
6208 TGTACA
BspMI 637 ACCTGC ACCTGC(4/8)
2677 ACCTGC
BsrFI 1594 ACCGGC [GA]^CCGG[CT]
2488 GCCGGC
BsrGI 820 TGTACA T^GTACA
6208 TGTACA
Bst1107I 429 GTATAC GTA^TAC
4673 GTATAC
BstZ17I 429 GTATAC GTA^TAC
4673 GTATAC
Cfr10I 1594 ACCGGC [GA]^CCGG[CT]
2488 GCCGGC
DraI 2070 TTTAAA TTT^AAA
6726 TTTAAA
EcoRI 2575 GAATTC G^AATTC
3605 GAATTC
EcoT22I 2962 ATGCAT ATGCA^T
6489 ATGCAT
HincII 1431 GTTGAC GT[CT]^[GA]AC
5014 GTCAAC
HindII 1431 GTTGAC GT[CT]^[GA]AC
5014 GTCAAC
MamI 2881 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3828 GATCTGGATC
NsiI 2962 ATGCAT ATGCA^T
6489 ATGCAT
SpeI 3510 ACTAGT A^CTAGT
5619 ACTAGT
SspBI 820 TGTACA T^GTACA
6208 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspLU11I BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI