##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
BciVI BsaBI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrGI BssHII BstEII
BstPI BstUI ClaI CpoI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FseI
KpnI KspI MamI MluI MvnI
NdeI NotI NruI NsiI PacI
PaeR7I PinAI PmeI PshBI RsrII
SacII SalI SbfI ScaI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 3136 GACGTC GACGT^C
AccIII 7421 TCCGGA T^CCGGA
AclI 286 AACGTT AA^CGTT
BamHI 988 GGATCC G^GATCC
BseAI 7421 TCCGGA T^CCGGA
BspEI 7421 TCCGGA T^CCGGA
Bst1107I 3782 GTATAC GTA^TAC
BstBI 2259 TTCGAA TT^CGAA
BstZ17I 3782 GTATAC GTA^TAC
DraIII 4557 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 6822 CGGCCG C^GGCCG
EclXI 6822 CGGCCG C^GGCCG
Eco52I 6822 CGGCCG C^GGCCG
EcoRI 6515 GAATTC G^AATTC
HincII 283 GTTAAC GT[CT]^[GA]AC
HindII 283 GTTAAC GT[CT]^[GA]AC
HpaI 283 GTTAAC GTT^AAC
MroI 7421 TCCGGA T^CCGGA
NheI 4165 GCTAGC G^CTAGC
NspV 2259 TTCGAA TT^CGAA
Psp1406I 286 AACGTT AA^CGTT
PvuI 1306 CGATCG CGAT^CG
SapI 7661 GCTCTTC GCTCTTC(1/4)
SexAI 7363 ACCAGGT A^CC[AT]GGT
SfuI 2259 TTCGAA TT^CGAA
SmaI 4282 CCCGGG CCC^GGG
SphI 1417 GCATGC GCATG^C
XmaCI 4282 CCCGGG C^CCGGG
XmaI 4282 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 1038 CTTAAG C^TTAAG
4941 CTTAAG
AocI 1097 CCTAAGG CC^T[ACGT]AGG
3613 CCTCAGG
AspI 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4038 GACTCAGTC
AviII 1142 TGCGCA TGC^GCA
4197 TGCGCA
AvrII 446 CCTAGG C^CTAGG
4764 CCTAGG
BbeI 1176 GGCGCC GGCGC^C
1231 GGCGCC
BbsI 7706 GAAGAC GAAGAC(2/6)
6568 GTCTTC
BcgI 3054 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5320 GCAAGTGGTTCG
BfrI 1038 CTTAAG C^TTAAG
4941 CTTAAG
BglII 2586 AGATCT A^GATCT
5167 AGATCT
BlnI 446 CCTAGG C^CTAGG
4764 CCTAGG
BpuAI 7706 GAAGAC GAAGAC(2/6)
6568 GTCTTC
BsiEI 1306 CGATCG CG[GA][CT]^CG
6822 CGGCCG
BspHI 2833 TCATGA T^CATGA
6994 TCATGA
BsrDI 3217 CATTGC GCAATG(2/0)
7630 GCAATG
Bsu36I 1097 CCTAAGG CC^T[ACGT]AGG
3613 CCTCAGG
DraI 6397 TTTAAA TTT^AAA
7269 TTTAAA
DrdI 763 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3179 GACGCCAAAGTC
Eco81I 1097 CCTAAGG CC^T[ACGT]AGG
3613 CCTCAGG
FspI 1142 TGCGCA TGC^GCA
4197 TGCGCA
KasI 1176 GGCGCC G^GCGCC
1231 GGCGCC
MfeI 6470 CAATTG C^AATTG
7319 CAATTG
MunI 6470 CAATTG C^AATTG
7319 CAATTG
NarI 1176 GGCGCC GG^CGCC
1231 GGCGCC
PflFI 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4038 GACTCAGTC
PshAI 117 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3244 GACCCTGGTC
RcaI 2833 TCATGA T^CATGA
6994 TCATGA
SacI 5135 GAGCTC GAGCT^C
6367 GAGCTC
SfoI 1176 GGCGCC GGC^GCC
1231 GGCGCC
SspI 4884 AATATT AAT^ATT
4906 AATATT
Tth111I 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4038 GACTCAGTC
XbaI 1533 TCTAGA T^CTAGA
1911 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvCI BbvI BclI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI