################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## Acc65I AccII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI BciVI BsaBI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BstEII BstPI BstUI ClaI CpoI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FseI KpnI KspI MamI MluI MvnI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI RsrII SacII SalI SbfI ScaI SfiI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 3136 GACGTC GACGT^C AccIII 7421 TCCGGA T^CCGGA AclI 286 AACGTT AA^CGTT BamHI 988 GGATCC G^GATCC BseAI 7421 TCCGGA T^CCGGA BspEI 7421 TCCGGA T^CCGGA Bst1107I 3782 GTATAC GTA^TAC BstBI 2259 TTCGAA TT^CGAA BstZ17I 3782 GTATAC GTA^TAC DraIII 4557 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 6822 CGGCCG C^GGCCG EclXI 6822 CGGCCG C^GGCCG Eco52I 6822 CGGCCG C^GGCCG EcoRI 6515 GAATTC G^AATTC HincII 283 GTTAAC GT[CT]^[GA]AC HindII 283 GTTAAC GT[CT]^[GA]AC HpaI 283 GTTAAC GTT^AAC MroI 7421 TCCGGA T^CCGGA NheI 4165 GCTAGC G^CTAGC NspV 2259 TTCGAA TT^CGAA Psp1406I 286 AACGTT AA^CGTT PvuI 1306 CGATCG CGAT^CG SapI 7661 GCTCTTC GCTCTTC(1/4) SexAI 7363 ACCAGGT A^CC[AT]GGT SfuI 2259 TTCGAA TT^CGAA SmaI 4282 CCCGGG CCC^GGG SphI 1417 GCATGC GCATG^C XmaCI 4282 CCCGGG C^CCGGG XmaI 4282 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AflII 1038 CTTAAG C^TTAAG 4941 CTTAAG AocI 1097 CCTAAGG CC^T[ACGT]AGG 3613 CCTCAGG AspI 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4038 GACTCAGTC AviII 1142 TGCGCA TGC^GCA 4197 TGCGCA AvrII 446 CCTAGG C^CTAGG 4764 CCTAGG BbeI 1176 GGCGCC GGCGC^C 1231 GGCGCC BbsI 7706 GAAGAC GAAGAC(2/6) 6568 GTCTTC BcgI 3054 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5320 GCAAGTGGTTCG BfrI 1038 CTTAAG C^TTAAG 4941 CTTAAG BglII 2586 AGATCT A^GATCT 5167 AGATCT BlnI 446 CCTAGG C^CTAGG 4764 CCTAGG BpuAI 7706 GAAGAC GAAGAC(2/6) 6568 GTCTTC BsiEI 1306 CGATCG CG[GA][CT]^CG 6822 CGGCCG BspHI 2833 TCATGA T^CATGA 6994 TCATGA BsrDI 3217 CATTGC GCAATG(2/0) 7630 GCAATG Bsu36I 1097 CCTAAGG CC^T[ACGT]AGG 3613 CCTCAGG DraI 6397 TTTAAA TTT^AAA 7269 TTTAAA DrdI 763 GACTTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3179 GACGCCAAAGTC Eco81I 1097 CCTAAGG CC^T[ACGT]AGG 3613 CCTCAGG FspI 1142 TGCGCA TGC^GCA 4197 TGCGCA KasI 1176 GGCGCC G^GCGCC 1231 GGCGCC MfeI 6470 CAATTG C^AATTG 7319 CAATTG MunI 6470 CAATTG C^AATTG 7319 CAATTG NarI 1176 GGCGCC GG^CGCC 1231 GGCGCC PflFI 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4038 GACTCAGTC PshAI 117 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3244 GACCCTGGTC RcaI 2833 TCATGA T^CATGA 6994 TCATGA SacI 5135 GAGCTC GAGCT^C 6367 GAGCTC SfoI 1176 GGCGCC GGC^GCC 1231 GGCGCC SspI 4884 AATATT AAT^ATT 4906 AATATT Tth111I 1883 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4038 GACTCAGTC XbaI 1533 TCTAGA T^CTAGA 1911 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI