##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI BbrPI BcgI BclI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
Eco47III EcoRV FbaI FseI KpnI
MfeI MluI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 2888 CTTAAG C^TTAAG
AspI 3524 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1138 TGCGCA TGC^GCA
BamHI 1564 GGATCC G^GATCC
BfrI 2888 CTTAAG C^TTAAG
BsaAI 3400 TACGTG [CT]AC^GT[GA]
BsaBI 3857 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 94 CGGCCG CG[GA][CT]^CG
BsmI 973 GCATTC GAATGC(1/-1)
Bsp1407I 2210 TGTACA T^GTACA
BsrFI 172 ACCGGC [GA]^CCGG[CT]
BsrGI 2210 TGTACA T^GTACA
BstEII 87 GGTTACC G^GT[ACGT]ACC
BstPI 87 GGTTACC G^GT[ACGT]ACC
Cfr10I 172 ACCGGC [GA]^CCGG[CT]
DraIII 3000 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4627 GACATATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 94 CGGCCG C^GGCCG
EclXI 94 CGGCCG C^GGCCG
Eco52I 94 CGGCCG C^GGCCG
EcoNI 476 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 87 GGTTACC G^GT[ACGT]ACC
EcoRI 5989 GAATTC G^AATTC
FspI 1138 TGCGCA TGC^GCA
HpaI 6346 GTTAAC GTT^AAC
KspI 1080 CCGCGG CCGC^GG
MamI 3857 GATAAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 3524 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 898 CTGCAG CTGCA^G
SacII 1080 CCGCGG CCGC^GG
ScaI 679 AGTACT AGT^ACT
SmaI 4056 CCCGGG CCC^GGG
SpeI 5540 ACTAGT A^CTAGT
SspBI 2210 TGTACA T^GTACA
Tth111I 3524 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4056 CCCGGG C^CCGGG
XmaI 4056 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 1861 TCCGGA T^CCGGA
2281 TCCGGA
AcyI 26 GGCGCC G[GA]^CG[CT]C
37 GGCGCC
AvrII 870 CCTAGG C^CTAGG
4500 CCTAGG
BbeI 26 GGCGCC GGCGC^C
37 GGCGCC
BglI 29 GCCCTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2038 GCCTGGTAGGC
BlnI 870 CCTAGG C^CTAGG
4500 CCTAGG
BlpI 360 GCTGAGC GC^T[ACGT]AGC
1833 GCTGAGC
Bpu1102I 360 GCTGAGC GC^T[ACGT]AGC
1833 GCTGAGC
BsaHI 26 GGCGCC G[GA]^CG[CT]C
37 GGCGCC
BseAI 1861 TCCGGA T^CCGGA
2281 TCCGGA
BsmBI 248 CGTCTC CGTCTC(1/5)
3983 GAGACG
BspEI 1861 TCCGGA T^CCGGA
2281 TCCGGA
BssSI 1260 CTCGTG CACGAG(-5/-1)
6286 CTCGTG
CelII 360 GCTGAGC GC^T[ACGT]AGC
1833 GCTGAGC
DraI 2379 TTTAAA TTT^AAA
5179 TTTAAA
HaeII 26 GGCGCC [GA]GCGC^[CT]
37 GGCGCC
Hin1I 26 GGCGCC G[GA]^CG[CT]C
37 GGCGCC
KasI 26 GGCGCC G^GCGCC
37 GGCGCC
MaeII 408 ACGT A^CGT
3401 ACGT
MroI 1861 TCCGGA T^CCGGA
2281 TCCGGA
NarI 26 GGCGCC GG^CGCC
37 GGCGCC
NdeI 829 CATATG CA^TATG
5231 CATATG
SacI 155 GAGCTC GAGCT^C
3221 GAGCTC
SexAI 3001 ACCAGGT A^CC[AT]GGT
4978 ACCTGGT
SfoI 26 GGCGCC GGC^GCC
37 GGCGCC
SphI 1634 GCATGC GCATG^C
4109 GCATGC
SspI 5904 AATATT AAT^ATT
6112 AATATT
StuI 56 AGGCCT AGG^CCT
473 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PshBI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI