##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI BbeI BbrPI BcgI
BciVI BglI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiEI BsiWI BsmI
BspHI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI Hin1I HindIII
HpaI KasI KpnI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 2008 CGCG CG^CG
AccIII 1098 TCCGGA T^CCGGA
AflII 486 CTTAAG C^TTAAG
AflIII 849 ACATGT A^C[GA][CT]GT
Aor51HI 2409 AGCGCT AGC^GCT
AspI 2218 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2028 TGCGCA TGC^GCA
AvrII 560 CCTAGG C^CTAGG
BalI 2401 TGGCCA TGG^CCA
BamHI 1326 GGATCC G^GATCC
BclI 1653 TGATCA T^GATCA
BfrI 486 CTTAAG C^TTAAG
BglII 1587 AGATCT A^GATCT
BlnI 560 CCTAGG C^CTAGG
BsaAI 172 CACGTA [CT]AC^GT[GA]
BseAI 1098 TCCGGA T^CCGGA
BsmBI 104 GAGACG CGTCTC(1/5)
Bsp1407I 1337 TGTACA T^GTACA
BspDI 2810 ATCGAT AT^CGAT
BspEI 1098 TCCGGA T^CCGGA
BspLU11I 849 ACATGT A^CATGT
BsrGI 1337 TGTACA T^GTACA
BstAPI 2149 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2705 GGTAACC G^GT[ACGT]ACC
BstPI 2705 GGTAACC G^GT[ACGT]ACC
BstUI 2008 CGCG CG^CG
ClaI 2810 ATCGAT AT^CGAT
Eco47III 2409 AGCGCT AGC^GCT
EcoO65I 2705 GGTAACC G^GT[ACGT]ACC
FbaI 1653 TGATCA T^GATCA
FspI 2028 TGCGCA TGC^GCA
HaeII 2409 AGCGCT [GA]GCGC^[CT]
HgaI 1571 GACGC GACGC(5/10)
KspI 2007 CCGCGG CCGC^GG
MaeII 173 ACGT A^CGT
MluNI 2401 TGGCCA TGG^CCA
MroI 1098 TCCGGA T^CCGGA
MscI 2401 TGGCCA TGG^CCA
MvnI 2008 CGCG CG^CG
NdeI 363 CATATG CA^TATG
PaeR7I 1842 CTCGAG C^TCGAG
PflFI 2218 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 2007 CCGCGG CCGC^GG
ScaI 329 AGTACT AGT^ACT
SmaI 33 CCCGGG CCC^GGG
SphI 2427 GCATGC GCATG^C
SspBI 1337 TGTACA T^GTACA
Tth111I 2218 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1842 CTCGAG C^TCGAG
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 348 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2766 CAGAGGCTG
Asp700I 1084 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAATCCATTC
AvaI 33 CCCGGG C^[CT]CG[GA]G
1842 CTCGAG
BanI 61 GGTGCC G^G[CT][GA]CC
1036 GGTGCC
BsaWI 1098 TCCGGA [AT]^CCGG[AT]
1323 ACCGGA
BsoBI 33 CCCGGG C^[CT]CG[GA]G
1842 CTCGAG
BstXI 862 CCAGGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2484 CCAGCCCCATGG
CfoI 2029 GCGC GCG^C
2410 GCGC
DrdI 704 GACCGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1052 GACCAGGATGTC
EaeI 1848 CGGCCA [CT]^GGCC[GA]
2401 TGGCCA
EcoNI 441 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2516 CCTGCTGCAGG
HhaI 2029 GCGC GCG^C
2410 GCGC
HinP1I 2029 GCGC G^CGC
2410 GCGC
HincII 1046 GTCAAC GT[CT]^[GA]AC
1448 GTTGAC
HindII 1046 GTCAAC GT[CT]^[GA]AC
1448 GTTGAC
SacI 1157 GAGCTC GAGCT^C
2792 GAGCTC
SapI 2789 GAAGAGC GCTCTTC(1/4)
2310 GCTCTTC
XcmI 582 CCATGGCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1851 CCAACGGGGAGGTGG
XmnI 1084 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAATCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII