##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI AviII BanII BbrPI BcgI
BciVI BglI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HgaI KspI MluI
MroI NaeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SphI Sse8387I Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1036 GGTACC G^GTACC
AcyI 6694 GGCGCC G[GA]^CG[CT]C
AocI 4410 CCTTAGG CC^T[ACGT]AGG
AseI 6393 ATTAAT AT^TAAT
AsnI 6393 ATTAAT AT^TAAT
Asp718I 1036 GGTACC G^GTACC
AvaI 6714 CTCGGG C^[CT]CG[GA]G
BbeI 6694 GGCGCC GGCGC^C
BbvCI 6722 GCTGAGG CCTCAGC(-5/-2)
BlpI 5204 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 5204 GCTTAGC GC^T[ACGT]AGC
BsaAI 1595 TACGTA [CT]AC^GT[GA]
BsaHI 6694 GGCGCC G[GA]^CG[CT]C
BsgI 4100 GTGCAG GTGCAG(16/14)
BsoBI 6714 CTCGGG C^[CT]CG[GA]G
BsrBI 6580 GAGCGG CCGCTC(-3/-3)
BstAPI 1294 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1982 TTCGAA TT^CGAA
Bsu36I 4410 CCTTAGG CC^T[ACGT]AGG
CelII 5204 GCTTAGC GC^T[ACGT]AGC
DrdI 5175 GACTATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 5933 CCATGG C^C[GA][CT]GG
Eco81I 4410 CCTTAGG CC^T[ACGT]AGG
HaeII 6694 GGCGCC [GA]GCGC^[CT]
Hin1I 6694 GGCGCC G[GA]^CG[CT]C
KasI 6694 GGCGCC G^GCGCC
KpnI 1036 GGTACC GGTAC^C
NarI 6694 GGCGCC GG^CGCC
NcoI 5933 CCATGG C^CATGG
NspV 1982 TTCGAA TT^CGAA
PflMI 5036 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 6393 ATTAAT AT^TAAT
SfoI 6694 GGCGCC GGC^GCC
SfuI 1982 TTCGAA TT^CGAA
SnaBI 1595 TACGTA TAC^GTA
SpeI 2070 ACTAGT A^CTAGT
SwaI 2936 ATTTAAAT ATTT^AAAT
Van91I 5036 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 5690 CGCG CG^CG
6534 CGCG
Alw44I 4490 GTGCAC G^TGCAC
5439 GTGCAC
ApaLI 4490 GTGCAC G^TGCAC
5439 GTGCAC
AvrII 132 CCTAGG C^CTAGG
4734 CCTAGG
BamHI 1248 GGATCC G^GATCC
5652 GGATCC
BanI 1036 GGTACC G^G[CT][GA]CC
6694 GGCGCC
BcnI 4304 CCCGG CC^[GC]GG
6679 CCGGG
BglII 877 AGATCT A^GATCT
5796 AGATCT
BlnI 132 CCTAGG C^CTAGG
4734 CCTAGG
BseRI 1505 GAGGAG GAGGAG(10/8)
5265 GAGGAG
BsmI 6084 GAATGC GAATGC(1/-1)
6100 GAATGC
BspLU11I 2724 ACATGT A^CATGT
5745 ACATGT
BstUI 5690 CGCG CG^CG
6534 CGCG
EcoNI 2485 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2896 CCTTCTTCAGG
EcoRI 1442 GAATTC G^AATTC
3547 GAATTC
EcoT22I 497 ATGCAT ATGCA^T
2210 ATGCAT
FauI 6691 GCGGG CCCGC(4/6)
6728 GCGGG
HincII 5473 GTTAAC GT[CT]^[GA]AC
5967 GTTAAC
HindII 5473 GTTAAC GT[CT]^[GA]AC
5967 GTTAAC
HpaI 5473 GTTAAC GTT^AAC
5967 GTTAAC
MfeI 1314 CAATTG C^AATTG
4696 CAATTG
MunI 1314 CAATTG C^AATTG
4696 CAATTG
MvnI 5690 CGCG CG^CG
6534 CGCG
NciI 4304 CCCGG CC^[GC]GG
6679 CCGGG
NsiI 497 ATGCAT ATGCA^T
2210 ATGCAT
PshAI 2638 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3400 GACTGGTGTC
PstI 398 CTGCAG CTGCA^G
5347 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BbsI BbvI
BclI BfaI BfrI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII
XmnI