################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AccI AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AviII BanII BbrPI BcgI BciVI BglI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HgaI KspI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmaI SphI Sse8387I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 1036 GGTACC G^GTACC AcyI 6694 GGCGCC G[GA]^CG[CT]C AocI 4410 CCTTAGG CC^T[ACGT]AGG AseI 6393 ATTAAT AT^TAAT AsnI 6393 ATTAAT AT^TAAT Asp718I 1036 GGTACC G^GTACC AvaI 6714 CTCGGG C^[CT]CG[GA]G BbeI 6694 GGCGCC GGCGC^C BbvCI 6722 GCTGAGG CCTCAGC(-5/-2) BlpI 5204 GCTTAGC GC^T[ACGT]AGC Bpu1102I 5204 GCTTAGC GC^T[ACGT]AGC BsaAI 1595 TACGTA [CT]AC^GT[GA] BsaHI 6694 GGCGCC G[GA]^CG[CT]C BsgI 4100 GTGCAG GTGCAG(16/14) BsoBI 6714 CTCGGG C^[CT]CG[GA]G BsrBI 6580 GAGCGG CCGCTC(-3/-3) BstAPI 1294 GCAACATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1982 TTCGAA TT^CGAA Bsu36I 4410 CCTTAGG CC^T[ACGT]AGG CelII 5204 GCTTAGC GC^T[ACGT]AGC DrdI 5175 GACTATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 5933 CCATGG C^C[GA][CT]GG Eco81I 4410 CCTTAGG CC^T[ACGT]AGG HaeII 6694 GGCGCC [GA]GCGC^[CT] Hin1I 6694 GGCGCC G[GA]^CG[CT]C KasI 6694 GGCGCC G^GCGCC KpnI 1036 GGTACC GGTAC^C NarI 6694 GGCGCC GG^CGCC NcoI 5933 CCATGG C^CATGG NspV 1982 TTCGAA TT^CGAA PflMI 5036 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 6393 ATTAAT AT^TAAT SfoI 6694 GGCGCC GGC^GCC SfuI 1982 TTCGAA TT^CGAA SnaBI 1595 TACGTA TAC^GTA SpeI 2070 ACTAGT A^CTAGT SwaI 2936 ATTTAAAT ATTT^AAAT Van91I 5036 CCACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 5690 CGCG CG^CG 6534 CGCG Alw44I 4490 GTGCAC G^TGCAC 5439 GTGCAC ApaLI 4490 GTGCAC G^TGCAC 5439 GTGCAC AvrII 132 CCTAGG C^CTAGG 4734 CCTAGG BamHI 1248 GGATCC G^GATCC 5652 GGATCC BanI 1036 GGTACC G^G[CT][GA]CC 6694 GGCGCC BcnI 4304 CCCGG CC^[GC]GG 6679 CCGGG BglII 877 AGATCT A^GATCT 5796 AGATCT BlnI 132 CCTAGG C^CTAGG 4734 CCTAGG BseRI 1505 GAGGAG GAGGAG(10/8) 5265 GAGGAG BsmI 6084 GAATGC GAATGC(1/-1) 6100 GAATGC BspLU11I 2724 ACATGT A^CATGT 5745 ACATGT BstUI 5690 CGCG CG^CG 6534 CGCG EcoNI 2485 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2896 CCTTCTTCAGG EcoRI 1442 GAATTC G^AATTC 3547 GAATTC EcoT22I 497 ATGCAT ATGCA^T 2210 ATGCAT FauI 6691 GCGGG CCCGC(4/6) 6728 GCGGG HincII 5473 GTTAAC GT[CT]^[GA]AC 5967 GTTAAC HindII 5473 GTTAAC GT[CT]^[GA]AC 5967 GTTAAC HpaI 5473 GTTAAC GTT^AAC 5967 GTTAAC MfeI 1314 CAATTG C^AATTG 4696 CAATTG MunI 1314 CAATTG C^AATTG 4696 CAATTG MvnI 5690 CGCG CG^CG 6534 CGCG NciI 4304 CCCGG CC^[GC]GG 6679 CCGGG NsiI 497 ATGCAT ATGCA^T 2210 ATGCAT PshAI 2638 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3400 GACTGGTGTC PstI 398 CTGCAG CTGCA^G 5347 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BbsI BbvI BclI BfaI BfrI BmyI BpmI BpuAI BsaBI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII XmnI