##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
ApaI AscI Asp718I AspEI AvaI
BbeI BglI BglII BlpI Bpu1102I
BsaBI BsaI BsiEI BsmI BsoBI
BspDI BsrBI BssHII BstBI BstUI
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco52I FseI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PaeR7I
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SphI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 18 GACGTC GACGT^C
AcyI 18 GACGTC G[GA]^CG[CT]C
Alw44I 3437 GTGCAC G^TGCAC
Aor51HI 3391 AGCGCT AGC^GCT
ApaLI 3437 GTGCAC G^TGCAC
AseI 4677 ATTAAT AT^TAAT
AsnI 4677 ATTAAT AT^TAAT
AviII 2730 TGCGCA TGC^GCA
AvrII 6868 CCTAGG C^CTAGG
BanII 3720 GAGCTC G[GA]GC[CT]^C
BbrPI 726 CACGTG CAC^GTG
BcnI 2248 CCCGG CC^[GC]GG
BlnI 6868 CCTAGG C^CTAGG
BsaHI 18 GACGTC G[GA]^CG[CT]C
BsiWI 7006 CGTACG C^GTACG
Bsp1407I 4817 TGTACA T^GTACA
BsrFI 525 ACCGGC [GA]^CCGG[CT]
BsrGI 4817 TGTACA T^GTACA
BssSI 902 CACGAG CACGAG(-5/-1)
BstAPI 6873 GCAAACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 499 GGTGACC G^GT[ACGT]ACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
Cfr10I 525 ACCGGC [GA]^CCGG[CT]
DrdI 2548 GACTGGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3391 AGCGCT AGC^GCT
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
EcoRV 1961 GATATC GAT^ATC
FauI 4794 GCGGG CCCGC(4/6)
FspI 2730 TGCGCA TGC^GCA
Hin1I 18 GACGTC G[GA]^CG[CT]C
NciI 2248 CCCGG CC^[GC]GG
PacI 5108 TTAATTAA TTAAT^TAA
PmaCI 726 CACGTG CAC^GTG
PmlI 726 CACGTG CAC^GTG
PpuMI 874 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 4677 ATTAAT AT^TAAT
SacI 3720 GAGCTC GAGCT^C
SbfI 2755 CCTGCAGG CCTGCA^GG
SnaBI 6841 TACGTA TAC^GTA
SpeI 2033 ACTAGT A^CTAGT
Sse8387I 2755 CCTGCAGG CCTGCA^GG
SspBI 4817 TGTACA T^GTACA
StuI 469 AGGCCT AGG^CCT
XbaI 5516 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 516 TCCGGA T^CCGGA
5961 TCCGGA
AciI 1572 CCGC CCGC(-3/-1)
4794 GCGG
AocI 878 CCTAAGG CC^T[ACGT]AGG
5796 CCTGAGG
BanI 824 GGTGCC G^G[CT][GA]CC
6630 GGCACC
BcgI 982 CGACCTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1889 GCATTTTTGTCG
BciVI 775 GGATAC GTATCC(6/5)
1141 GGATAC
BsaAI 726 CACGTG [CT]AC^GT[GA]
6841 TACGTA
BseAI 516 TCCGGA T^CCGGA
5961 TCCGGA
BsmBI 3358 CGTCTC CGTCTC(1/5)
16 GAGACG
BspEI 516 TCCGGA T^CCGGA
5961 TCCGGA
BspLU11I 377 ACATGT A^CATGT
1860 ACATGT
Bsu36I 878 CCTAAGG CC^T[ACGT]AGG
5796 CCTGAGG
DraII 874 AGGTCCT [GA]G^G[ACGT]CC[CT]
3969 GGGGCCT
Eco81I 878 CCTAAGG CC^T[ACGT]AGG
5796 CCTGAGG
EcoNI 792 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4045 CCTGCAGCAGG
EcoO109I 874 AGGTCCT [GA]G^G[ACGT]CC[CT]
3969 GGGGCCT
EcoRI 6195 GAATTC G^AATTC
6857 GAATTC
EcoT22I 5416 ATGCAT ATGCA^T
7096 ATGCAT
HaeII 1249 AGCGCC [GA]GCGC^[CT]
3391 AGCGCT
HgaI 6138 GACGC GACGC(5/10)
2716 GCGTC
HpaI 5773 GTTAAC GTT^AAC
6009 GTTAAC
MroI 516 TCCGGA T^CCGGA
5961 TCCGGA
MspA1I 695 CAGCTG C[AC]G^C[GT]G
2295 CAGCTG
NcoI 2010 CCATGG C^CATGG
4437 CCATGG
NdeI 4543 CATATG CA^TATG
4581 CATATG
NsiI 5416 ATGCAT ATGCA^T
7096 ATGCAT
PflMI 2201 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5300 CCAAATTCTGG
PvuII 695 CAGCTG CAG^CTG
2295 CAGCTG
ScaI 4743 AGTACT AGT^ACT
6413 AGTACT
TaqI 1898 TCGA T^CGA
2445 TCGA
TthHB8I 1898 TCGA T^CGA
2445 TCGA
Van91I 2201 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5300 CCAAATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AspI AvaII BalI BamHI
BbsI BbvCI BbvI BclI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI Bst1107I
BstF5I BstNI BstXI BstYI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI XhoII XmnI