##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI AocI Aor51HI ApaI AscI
Asp718I AspEI AvaI AviII AvrII
BbrPI BclI BcnI BglI BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BssHII BstBI BstEII BstPI
BstUI Bsu36I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FbaI
FseI FspI HapII HindIII HpaII
KpnI KspI MfeI MluI MroI
MspI MunI MvnI NaeI NciI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 408 GACGTC GACGT^C
AspI 2583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2053 TGGCCA TGG^CCA
BamHI 6032 GGATCC G^GATCC
BanI 5793 GGCGCC G^G[CT][GA]CC
BbeI 5793 GGCGCC GGCGC^C
BcgI 5012 GCAGTTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3026 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3026 GCTGAGC GC^T[ACGT]AGC
BsaAI 3733 TACGTG [CT]AC^GT[GA]
BsaI 2391 GAGACC GGTCTC(1/5)
BspLU11I 3404 ACATGT A^CATGT
BssSI 396 CACGAG CACGAG(-5/-1)
BstAPI 6348 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3026 GCTGAGC GC^T[ACGT]AGC
CfoI 5794 GCGC GCG^C
EaeI 2053 TGGCCA [CT]^GGCC[GA]
EcoRI 107 GAATTC G^AATTC
FauI 2213 CCCGC CCCGC(4/6)
HaeII 5793 GGCGCC [GA]GCGC^[CT]
HgaI 3023 GACGC GACGC(5/10)
HhaI 5794 GCGC GCG^C
HinP1I 5794 GCGC G^CGC
KasI 5793 GGCGCC G^GCGCC
MluNI 2053 TGGCCA TGG^CCA
MscI 2053 TGGCCA TGG^CCA
NarI 5793 GGCGCC GG^CGCC
NdeI 6688 CATATG CA^TATG
PflFI 2583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 5224 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 5010 CTGCAG CTGCA^G
SalI 148 GTCGAC G^TCGAC
SapI 3555 GAAGAGC GCTCTTC(1/4)
SfoI 5793 GGCGCC GGC^GCC
SphI 3609 GCATGC GCATG^C
StuI 1608 AGGCCT AGG^CCT
Tth111I 2583 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1893 CCAAAATAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 408 GACGTC G[GA]^CG[CT]C
5793 GGCGCC
AflII 4281 CTTAAG C^TTAAG
4626 CTTAAG
AflIII 3404 ACATGT A^C[GA][CT]GT
3734 ACGTGT
Alw44I 3891 GTGCAC G^TGCAC
6504 GTGCAC
AlwNI 275 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3021 CAGACGCTG
ApaLI 3891 GTGCAC G^TGCAC
6504 GTGCAC
BfrI 4281 CTTAAG C^TTAAG
4626 CTTAAG
BglII 2733 AGATCT A^GATCT
5955 AGATCT
BsaBI 2728 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3040 GATTAGCATC
BsaHI 408 GACGTC G[GA]^CG[CT]C
5793 GGCGCC
BseRI 5622 GAGGAG GAGGAG(10/8)
5625 GAGGAG
BsmFI 2474 GTCCC GGGAC(10/14)
4441 GTCCC
BsrDI 451 CATTGC GCAATG(2/0)
2764 CATTGC
Bst1107I 77 GTATAC GTA^TAC
4200 GTATAC
BstZ17I 77 GTATAC GTA^TAC
4200 GTATAC
DsaI 15 CCATGG C^C[GA][CT]GG
4271 CCATGG
EcoRV 4597 GATATC GAT^ATC
6213 GATATC
EcoT22I 3233 ATGCAT ATGCA^T
6763 ATGCAT
Hin1I 408 GACGTC G[GA]^CG[CT]C
5793 GGCGCC
MamI 2728 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3040 GATTAGCATC
NcoI 15 CCATGG C^CATGG
4271 CCATGG
NheI 3832 GCTAGC G^CTAGC
5382 GCTAGC
NsiI 3233 ATGCAT ATGCA^T
6763 ATGCAT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvCI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HpaI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XhoII XmnI