##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp718I AspI AviII AvrII
BbeI BclI BlnI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
Cfr10I ClaI CpoI EagI EclXI
Eco52I EcoRV FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MluI MroI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfoI
SgrAI SnaBI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccII 173 CGCG CG^CG
AflII 973 CTTAAG C^TTAAG
AflIII 2102 ACATGT A^C[GA][CT]GT
AhdI 6165 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5456 AGCGCT AGC^GCT
AspEI 6165 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 6079 CACGTG CAC^GTG
BfrI 973 CTTAAG C^TTAAG
BglII 4016 AGATCT A^GATCT
BsaAI 6079 CACGTG [CT]AC^GT[GA]
BsaWI 3644 ACCGGA [AT]^CCGG[AT]
BspLU11I 2102 ACATGT A^CATGT
BsrBI 2126 GAGCGG CCGCTC(-3/-3)
BssSI 3806 CTCGTG CACGAG(-5/-1)
BstBI 1985 TTCGAA TT^CGAA
BstUI 173 CGCG CG^CG
Eam1105I 6165 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5456 AGCGCT AGC^GCT
MvnI 173 CGCG CG^CG
NheI 7378 GCTAGC G^CTAGC
NspV 1985 TTCGAA TT^CGAA
PmaCI 6079 CACGTG CAC^GTG
PmlI 6079 CACGTG CAC^GTG
PshAI 4479 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2477 GAGCTC GAGCT^C
ScaI 3340 AGTACT AGT^ACT
SfiI 561 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1985 TTCGAA TT^CGAA
SspI 7853 AATATT AAT^ATT
XbaI 4152 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 321 GGCGTC G[GA]^CG[CT]C
5850 GACGCC
AocI 203 CCTGAGG CC^T[ACGT]AGG
7664 CCTGAGG
ApaI 24 GGGCCC GGGCC^C
6007 GGGCCC
BcgI 6206 CGACAGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2982 GCAGCAGCCTCG
BsaBI 427 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5089 GATCCTGATC
BsaHI 321 GGCGTC G[GA]^CG[CT]C
5850 GACGCC
BsmBI 6058 GAGACG CGTCTC(1/5)
6798 GAGACG
Bsp1407I 1465 TGTACA T^GTACA
3988 TGTACA
BspHI 821 TCATGA T^CATGA
4491 TCATGA
BsrGI 1465 TGTACA T^GTACA
3988 TGTACA
Bst1107I 3661 GTATAC GTA^TAC
4426 GTATAC
BstEII 5961 GGTGACC G^GT[ACGT]ACC
7708 GGTTACC
BstPI 5961 GGTGACC G^GT[ACGT]ACC
7708 GGTTACC
BstZ17I 3661 GTATAC GTA^TAC
4426 GTATAC
Bsu36I 203 CCTGAGG CC^T[ACGT]AGG
7664 CCTGAGG
Eco81I 203 CCTGAGG CC^T[ACGT]AGG
7664 CCTGAGG
EcoO65I 5961 GGTGACC G^GT[ACGT]ACC
7708 GGTTACC
EcoT22I 4009 ATGCAT ATGCA^T
4375 ATGCAT
Hin1I 321 GGCGTC G[GA]^CG[CT]C
5850 GACGCC
MaeII 6080 ACGT A^CGT
7265 ACGT
MamI 427 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5089 GATCCTGATC
MfeI 833 CAATTG C^AATTG
5502 CAATTG
MunI 833 CAATTG C^AATTG
5502 CAATTG
NsiI 4009 ATGCAT ATGCA^T
4375 ATGCAT
RcaI 821 TCATGA T^CATGA
4491 TCATGA
SmaI 182 CCCGGG CCC^GGG
4960 CCCGGG
SpeI 667 ACTAGT A^CTAGT
3118 ACTAGT
SspBI 1465 TGTACA T^GTACA
3988 TGTACA
XmaCI 182 CCCGGG C^CCGGG
4960 CCCGGG
XmaI 182 CCCGGG C^CCGGG
4960 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DraIII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI