##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BglII BlnI BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI HincII HindII HpaI KpnI
KspI MluI MroI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacII
SalI SfiI SfuI SgrAI SmaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 210 CGCG CG^CG
AclI 4252 AACGTT AA^CGTT
AcyI 6292 GGCGCC G[GA]^CG[CT]C
Alw44I 1611 GTGCAC G^TGCAC
ApaLI 1611 GTGCAC G^TGCAC
AseI 276 ATTAAT AT^TAAT
AsnI 276 ATTAAT AT^TAAT
BbeI 6292 GGCGCC GGCGC^C
BcgI 160 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 9 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4094 TACGTA [CT]AC^GT[GA]
BsaBI 824 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6292 GGCGCC G[GA]^CG[CT]C
BsmBI 4959 GAGACG CGTCTC(1/5)
BsmI 6875 GAATGC GAATGC(1/-1)
BsrFI 58 ACCGGC [GA]^CCGG[CT]
BstUI 210 CGCG CG^CG
Cfr10I 58 ACCGGC [GA]^CCGG[CT]
EcoRV 6661 GATATC GAT^ATC
HaeII 6292 GGCGCC [GA]GCGC^[CT]
HgaI 7158 GCGTC GACGC(5/10)
Hin1I 6292 GGCGCC G[GA]^CG[CT]C
KasI 6292 GGCGCC G^GCGCC
MamI 824 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3132 CAATTG C^AATTG
MunI 3132 CAATTG C^AATTG
MvnI 210 CGCG CG^CG
NarI 6292 GGCGCC GG^CGCC
NdeI 4005 CATATG CA^TATG
NheI 2440 GCTAGC G^CTAGC
PpuMI 6897 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 276 ATTAAT AT^TAAT
Psp1406I 4252 AACGTT AA^CGTT
SapI 1505 GCTCTTC GCTCTTC(1/4)
SbfI 6902 CCTGCAGG CCTGCA^GG
SexAI 1680 ACCTGGT A^CC[AT]GGT
SfoI 6292 GGCGCC GGC^GCC
SnaBI 4094 TACGTA TAC^GTA
Sse8387I 6902 CCTGCAGG CCTGCA^GG
SwaI 6038 ATTTAAAT ATTT^AAAT
TaqI 1493 TCGA T^CGA
TthHB8I 1493 TCGA T^CGA
XcmI 2257 CCAGTGAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 804 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1377 CAGAGCCTG
AocI 38 CCTGAGG CC^T[ACGT]AGG
2013 CCTCAGG
ApaI 2707 GGGCCC GGGCC^C
2771 GGGCCC
AvaI 1187 CCCGAG C^[CT]CG[GA]G
6312 CTCGGG
BalI 65 TGGCCA TGG^CCA
6230 TGGCCA
BsaI 26 GGTCTC GGTCTC(1/5)
5131 GGTCTC
BsoBI 1187 CCCGAG C^[CT]CG[GA]G
6312 CTCGGG
BspHI 2456 TCATGA T^CATGA
4829 TCATGA
BspMI 5353 ACCTGC ACCTGC(4/8)
7185 GCAGGT
BssSI 6917 CACGAG CACGAG(-5/-1)
6032 CTCGTG
BstXI 3713 CCATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6172 CCAGCACTTTGG
Bsu36I 38 CCTGAGG CC^T[ACGT]AGG
2013 CCTCAGG
DsaI 839 CCATGG C^C[GA][CT]GG
3023 CCATGG
Eco81I 38 CCTGAGG CC^T[ACGT]AGG
2013 CCTCAGG
EcoNI 2882 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5895 CCTTCAAAAGG
MluNI 65 TGGCCA TGG^CCA
6230 TGGCCA
MscI 65 TGGCCA TGG^CCA
6230 TGGCCA
NcoI 839 CCATGG C^CATGG
3023 CCATGG
RcaI 2456 TCATGA T^CATGA
4829 TCATGA
SacI 2955 GAGCTC GAGCT^C
5025 GAGCTC
ScaI 861 AGTACT AGT^ACT
4758 AGTACT
SphI 125 GCATGC GCATG^C
787 GCATGC
StuI 2017 AGGCCT AGG^CCT
2192 AGGCCT
XbaI 4412 TCTAGA T^CTAGA
4732 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmnI