##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AciI AclI AcyI
AgeI AhdI AlwNI Aor51HI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BglI BlnI BsaHI BsiEI
BsiWI BsmBI BspDI BsrBI BsrFI
BssHII BssSI BstEII BstPI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccII 6175 CGCG CG^CG
AccIII 3558 TCCGGA T^CCGGA
AflII 4816 CTTAAG C^TTAAG
ApaI 3907 GGGCCC GGGCC^C
BamHI 2901 GGATCC G^GATCC
BanI 4064 GGCACC G^G[CT][GA]CC
BbrPI 364 CACGTG CAC^GTG
BcgI 3676 CGATTTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3555 GTATCC GTATCC(6/5)
BfrI 4816 CTTAAG C^TTAAG
BlpI 1924 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1924 GCTTAGC GC^T[ACGT]AGC
BsaAI 364 CACGTG [CT]AC^GT[GA]
BsaI 4306 GGTCTC GGTCTC(1/5)
BsaWI 3558 TCCGGA [AT]^CCGG[AT]
BseAI 3558 TCCGGA T^CCGGA
BspEI 3558 TCCGGA T^CCGGA
BspMI 5671 ACCTGC ACCTGC(4/8)
BstBI 5691 TTCGAA TT^CGAA
BstUI 6175 CGCG CG^CG
CelII 1924 GCTTAGC GC^T[ACGT]AGC
CfoI 6176 GCGC GCG^C
DsaI 348 CCATGG C^C[GA][CT]GG
HhaI 6176 GCGC GCG^C
HinP1I 6176 GCGC G^CGC
MroI 3558 TCCGGA T^CCGGA
MvnI 6175 CGCG CG^CG
NcoI 348 CCATGG C^CATGG
NspV 5691 TTCGAA TT^CGAA
PmaCI 364 CACGTG CAC^GTG
PmlI 364 CACGTG CAC^GTG
SfuI 5691 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1819 GTATAC GT^[AC][GT]AC
6282 GTATAC
AflIII 1401 ACATGT A^C[GA][CT]GT
2920 ACATGT
Asp700I 2627 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6020 GAATATTTTC
AvaI 114 CCCGGG C^[CT]CG[GA]G
146 CCCGGG
AvaII 5669 GGACC G^G[AT]CC
5843 GGTCC
BalI 2482 TGGCCA TGG^CCA
4250 TGGCCA
BbsI 473 GAAGAC GAAGAC(2/6)
1072 GAAGAC
BbvCI 4083 CCTCAGC CCTCAGC(-5/-2)
6527 CCTCAGC
BclI 2339 TGATCA T^GATCA
3504 TGATCA
BpuAI 473 GAAGAC GAAGAC(2/6)
1072 GAAGAC
BsaBI 1613 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2896 GATGAGGATC
BseRI 2400 GAGGAG GAGGAG(10/8)
3604 GAGGAG
BsoBI 114 CCCGGG C^[CT]CG[GA]G
146 CCCGGG
BspLU11I 1401 ACATGT A^CATGT
2920 ACATGT
Bst1107I 1819 GTATAC GTA^TAC
6282 GTATAC
BstZ17I 1819 GTATAC GTA^TAC
6282 GTATAC
DraII 3906 GGGGCCC [GA]G^G[ACGT]CC[CT]
3907 GGGCCCT
DraIII 2640 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5928 CACGTTGTG
EaeI 2482 TGGCCA [CT]^GGCC[GA]
4250 TGGCCA
EarI 1869 CTCTTC CTCTTC(1/4)
4309 CTCTTC
EcoNI 3401 CCTTGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4947 CCTTCAGTAGG
EcoO109I 3906 GGGGCCC [GA]G^G[ACGT]CC[CT]
3907 GGGCCCT
EcoRV 336 GATATC GAT^ATC
5373 GATATC
EcoT14I 348 CCATGG C^C[AT][AT]GG
3422 CCTTGG
EcoT22I 596 ATGCAT ATGCA^T
4132 ATGCAT
FbaI 2339 TGATCA T^GATCA
3504 TGATCA
Ksp632I 1869 CTCTTC CTCTTC(1/4)
4309 CTCTTC
MamI 1613 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2896 GATGAGGATC
MfeI 2136 CAATTG C^AATTG
5439 CAATTG
MluNI 2482 TGGCCA TGG^CCA
4250 TGGCCA
MscI 2482 TGGCCA TGG^CCA
4250 TGGCCA
MspA1I 561 CAGCTG C[AC]G^C[GT]G
3977 CAGCTG
MunI 2136 CAATTG C^AATTG
5439 CAATTG
NsiI 596 ATGCAT ATGCA^T
4132 ATGCAT
PvuII 561 CAGCTG CAG^CTG
3977 CAGCTG
SmaI 114 CCCGGG CCC^GGG
146 CCCGGG
SpeI 2300 ACTAGT A^CTAGT
5048 ACTAGT
StuI 833 AGGCCT AGG^CCT
3996 AGGCCT
StyI 348 CCATGG C^C[AT][AT]GG
3422 CCTTGG
XmaCI 114 CCCGGG C^CCGGG
146 CCCGGG
XmaI 114 CCCGGG C^CCGGG
146 CCCGGG
XmnI 2627 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6020 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AcsI AfaI AluI Alw44I AlwI
AocI ApaLI ApoI AseI AsnI
AspHI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI Eco81I
EcoRI EcoRII Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII