################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII AgeI Alw44I Aor51HI ApaLI AscI AvrII BbeI BcgI BglI BlnI BsaBI BsiEI BsiWI BsmBI BsrDI BsrFI BstBI BstEII BstPI Cfr10I CpoI EagI EclXI Eco47III Eco52I EcoO65I FseI KasI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PshAI PstI PvuI PvuII RsrII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 3412 GGTACC G^GTACC AclI 4855 AACGTT AA^CGTT AflII 3576 CTTAAG C^TTAAG AhdI 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 3818 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1082 GGGCCC GGGCC^C Asp700I 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3412 GGTACC G^GTACC AspEI 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3605 GGATCC G^GATCC BbsI 2538 GAAGAC GAAGAC(2/6) BbvCI 3721 CCTCAGC CCTCAGC(-5/-2) BciVI 2969 GGATAC GTATCC(6/5) BfrI 3576 CTTAAG C^TTAAG BglII 4730 AGATCT A^GATCT BpuAI 2538 GAAGAC GAAGAC(2/6) Bsp1407I 2750 TGTACA T^GTACA BspHI 5043 TCATGA T^CATGA BspMI 1221 GCAGGT ACCTGC(4/8) BsrGI 2750 TGTACA T^GTACA BssHII 100 GCGCGC G^CGCGC BstXI 4835 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 862 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3652 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 4220 AGCGCC [GA]GCGC^[CT] HpaI 2096 GTTAAC GTT^AAC KpnI 3412 GGTACC GGTAC^C KspI 4233 CCGCGG CCGC^GG MspA1I 4233 CCGCGG C[AC]G^C[GT]G NcoI 2965 CCATGG C^CATGG NdeI 1752 CATATG CA^TATG PaeR7I 763 CTCGAG C^TCGAG PflFI 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmeI 2037 GTTTAAAC GTTT^AAAC Psp1406I 4855 AACGTT AA^CGTT RcaI 5043 TCATGA T^CATGA SacII 4233 CCGCGG CCGC^GG SapI 6008 GAAGAGC GCTCTTC(1/4) SmaI 1362 CCCGGG CCC^GGG SpeI 3516 ACTAGT A^CTAGT SphI 1986 GCATGC GCATG^C SspBI 2750 TGTACA T^GTACA Tth111I 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3777 TCTAGA T^CTAGA XhoI 763 CTCGAG C^TCGAG XmaCI 1362 CCCGGG C^CCGGG XmaI 1362 CCCGGG C^CCGGG XmnI 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccIII 4387 TCCGGA T^CCGGA 5189 TCCGGA AcyI 1089 GACGCC G[GA]^CG[CT]C 4454 GACGCC AseI 2173 ATTAAT AT^TAAT 4447 ATTAAT AsnI 2173 ATTAAT AT^TAAT 4447 ATTAAT AvaI 763 CTCGAG C^[CT]CG[GA]G 1362 CCCGGG AviII 125 TGCGCA TGC^GCA 5093 TGCGCA BbrPI 933 CACGTG CAC^GTG 5564 CACGTG BlpI 4749 GCTCAGC GC^T[ACGT]AGC 4981 GCTAAGC Bpu1102I 4749 GCTCAGC GC^T[ACGT]AGC 4981 GCTAAGC BsaHI 1089 GACGCC G[GA]^CG[CT]C 4454 GACGCC BseAI 4387 TCCGGA T^CCGGA 5189 TCCGGA BsgI 3477 CTGCAC GTGCAG(16/14) 4331 GTGCAG BsoBI 763 CTCGAG C^[CT]CG[GA]G 1362 CCCGGG BspDI 976 ATCGAT AT^CGAT 1639 ATCGAT BspEI 4387 TCCGGA T^CCGGA 5189 TCCGGA BspLU11I 1437 ACATGT A^CATGT 2747 ACATGT BsrBI 108 CCGCTC CCGCTC(-3/-3) 18 GAGCGG BssSI 996 CACGAG CACGAG(-5/-1) 3533 CACGAG Bst1107I 2859 GTATAC GTA^TAC 3732 GTATAC BstAPI 3185 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6013 GCACAATATGC BstZ17I 2859 GTATAC GTA^TAC 3732 GTATAC CelII 4749 GCTCAGC GC^T[ACGT]AGC 4981 GCTAAGC ClaI 976 ATCGAT AT^CGAT 1639 ATCGAT EcoNI 3913 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4014 CCTGCAAGAGG EcoT22I 3290 ATGCAT ATGCA^T 6020 ATGCAT FspI 125 TGCGCA TGC^GCA 5093 TGCGCA Hin1I 1089 GACGCC G[GA]^CG[CT]C 4454 GACGCC HindIII 241 AAGCTT A^AGCTT 5468 AAGCTT MroI 4387 TCCGGA T^CCGGA 5189 TCCGGA NsiI 3290 ATGCAT ATGCA^T 6020 ATGCAT PflMI 4519 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5496 CCAGTTTGTGG PmaCI 933 CACGTG CAC^GTG 5564 CACGTG PmlI 933 CACGTG CAC^GTG 5564 CACGTG PshBI 2173 ATTAAT AT^TAAT 4447 ATTAAT SacI 1047 GAGCTC GAGCT^C 4554 GAGCTC SalI 5267 GTCGAC G^TCGAC 6392 GTCGAC SnaBI 3285 TACGTA TAC^GTA 6273 TACGTA Van91I 4519 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5496 CCAGTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AocI ApoI AspHI AvaII BalI BanI BanII BbvI BclI BcnI BfaI BmyI BpmI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII