##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AgeI Alw44I Aor51HI ApaLI
AscI AvrII BbeI BcgI BglI
BlnI BsaBI BsiEI BsiWI BsmBI
BsrDI BsrFI BstBI BstEII BstPI
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoO65I FseI KasI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PshAI PstI PvuI
PvuII RsrII SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI Sse8387I
StuI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 3412 GGTACC G^GTACC
AclI 4855 AACGTT AA^CGTT
AflII 3576 CTTAAG C^TTAAG
AhdI 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3818 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1082 GGGCCC GGGCC^C
Asp700I 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3412 GGTACC G^GTACC
AspEI 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3605 GGATCC G^GATCC
BbsI 2538 GAAGAC GAAGAC(2/6)
BbvCI 3721 CCTCAGC CCTCAGC(-5/-2)
BciVI 2969 GGATAC GTATCC(6/5)
BfrI 3576 CTTAAG C^TTAAG
BglII 4730 AGATCT A^GATCT
BpuAI 2538 GAAGAC GAAGAC(2/6)
Bsp1407I 2750 TGTACA T^GTACA
BspHI 5043 TCATGA T^CATGA
BspMI 1221 GCAGGT ACCTGC(4/8)
BsrGI 2750 TGTACA T^GTACA
BssHII 100 GCGCGC G^CGCGC
BstXI 4835 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 862 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3652 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 398 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 4220 AGCGCC [GA]GCGC^[CT]
HpaI 2096 GTTAAC GTT^AAC
KpnI 3412 GGTACC GGTAC^C
KspI 4233 CCGCGG CCGC^GG
MspA1I 4233 CCGCGG C[AC]G^C[GT]G
NcoI 2965 CCATGG C^CATGG
NdeI 1752 CATATG CA^TATG
PaeR7I 763 CTCGAG C^TCGAG
PflFI 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 2037 GTTTAAAC GTTT^AAAC
Psp1406I 4855 AACGTT AA^CGTT
RcaI 5043 TCATGA T^CATGA
SacII 4233 CCGCGG CCGC^GG
SapI 6008 GAAGAGC GCTCTTC(1/4)
SmaI 1362 CCCGGG CCC^GGG
SpeI 3516 ACTAGT A^CTAGT
SphI 1986 GCATGC GCATG^C
SspBI 2750 TGTACA T^GTACA
Tth111I 2487 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3777 TCTAGA T^CTAGA
XhoI 763 CTCGAG C^TCGAG
XmaCI 1362 CCCGGG C^CCGGG
XmaI 1362 CCCGGG C^CCGGG
XmnI 536 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 4387 TCCGGA T^CCGGA
5189 TCCGGA
AcyI 1089 GACGCC G[GA]^CG[CT]C
4454 GACGCC
AseI 2173 ATTAAT AT^TAAT
4447 ATTAAT
AsnI 2173 ATTAAT AT^TAAT
4447 ATTAAT
AvaI 763 CTCGAG C^[CT]CG[GA]G
1362 CCCGGG
AviII 125 TGCGCA TGC^GCA
5093 TGCGCA
BbrPI 933 CACGTG CAC^GTG
5564 CACGTG
BlpI 4749 GCTCAGC GC^T[ACGT]AGC
4981 GCTAAGC
Bpu1102I 4749 GCTCAGC GC^T[ACGT]AGC
4981 GCTAAGC
BsaHI 1089 GACGCC G[GA]^CG[CT]C
4454 GACGCC
BseAI 4387 TCCGGA T^CCGGA
5189 TCCGGA
BsgI 3477 CTGCAC GTGCAG(16/14)
4331 GTGCAG
BsoBI 763 CTCGAG C^[CT]CG[GA]G
1362 CCCGGG
BspDI 976 ATCGAT AT^CGAT
1639 ATCGAT
BspEI 4387 TCCGGA T^CCGGA
5189 TCCGGA
BspLU11I 1437 ACATGT A^CATGT
2747 ACATGT
BsrBI 108 CCGCTC CCGCTC(-3/-3)
18 GAGCGG
BssSI 996 CACGAG CACGAG(-5/-1)
3533 CACGAG
Bst1107I 2859 GTATAC GTA^TAC
3732 GTATAC
BstAPI 3185 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6013 GCACAATATGC
BstZ17I 2859 GTATAC GTA^TAC
3732 GTATAC
CelII 4749 GCTCAGC GC^T[ACGT]AGC
4981 GCTAAGC
ClaI 976 ATCGAT AT^CGAT
1639 ATCGAT
EcoNI 3913 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4014 CCTGCAAGAGG
EcoT22I 3290 ATGCAT ATGCA^T
6020 ATGCAT
FspI 125 TGCGCA TGC^GCA
5093 TGCGCA
Hin1I 1089 GACGCC G[GA]^CG[CT]C
4454 GACGCC
HindIII 241 AAGCTT A^AGCTT
5468 AAGCTT
MroI 4387 TCCGGA T^CCGGA
5189 TCCGGA
NsiI 3290 ATGCAT ATGCA^T
6020 ATGCAT
PflMI 4519 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5496 CCAGTTTGTGG
PmaCI 933 CACGTG CAC^GTG
5564 CACGTG
PmlI 933 CACGTG CAC^GTG
5564 CACGTG
PshBI 2173 ATTAAT AT^TAAT
4447 ATTAAT
SacI 1047 GAGCTC GAGCT^C
4554 GAGCTC
SalI 5267 GTCGAC G^TCGAC
6392 GTCGAC
SnaBI 3285 TACGTA TAC^GTA
6273 TACGTA
Van91I 4519 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5496 CCAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AocI ApoI
AspHI AvaII BalI BanI BanII
BbvI BclI BcnI BfaI BmyI
BpmI BsaAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoRV
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII