##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Aor51HI ApaI AscI AspI AviII
BbeI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DsaI
EagI EclXI Eco47III Eco52I EcoO65I
FseI FspI HaeII HgaI Hin1I
KasI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 7498 ACATGT A^C[GA][CT]GT
AhdI 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1709 GTGCAC G^TGCAC
ApaLI 1709 GTGCAC G^TGCAC
AspEI 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4178 TGGCCA TGG^CCA
BbrPI 3034 CACGTG CAC^GTG
BbvCI 2445 CCTCAGC CCTCAGC(-5/-2)
BciVI 5352 GTATCC GTATCC(6/5)
BclI 333 TGATCA T^GATCA
BglI 6951 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4624 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4624 GCTCAGC GC^T[ACGT]AGC
BsaAI 3034 CACGTG [CT]AC^GT[GA]
Bsp1407I 3823 TGTACA T^GTACA
BspDI 6941 ATCGAT AT^CGAT
BspHI 438 TCATGA T^CATGA
BspLU11I 7498 ACATGT A^CATGT
BsrGI 3823 TGTACA T^GTACA
BstAPI 1641 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4624 GCTCAGC GC^T[ACGT]AGC
ClaI 6941 ATCGAT AT^CGAT
Eam1105I 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1161 CCTCCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 6112 GAATTC G^AATTC
EcoT22I 5816 ATGCAT ATGCA^T
FbaI 333 TGATCA T^GATCA
HpaI 5903 GTTAAC GTT^AAC
MluNI 4178 TGGCCA TGG^CCA
MscI 4178 TGGCCA TGG^CCA
NsiI 5816 ATGCAT ATGCA^T
PmaCI 3034 CACGTG CAC^GTG
PmlI 3034 CACGTG CAC^GTG
RcaI 438 TCATGA T^CATGA
SexAI 3421 ACCTGGT A^CC[AT]GGT
SpeI 5841 ACTAGT A^CTAGT
SspBI 3823 TGTACA T^GTACA
StuI 6958 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 152 GTCTAC GT^[AC][GT]AC
2861 GTCTAC
AccII 1809 CGCG CG^CG
7530 CGCG
AflII 887 CTTAAG C^TTAAG
6170 CTTAAG
AvrII 916 CCTAGG C^CTAGG
1934 CCTAGG
BcgI 1666 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2434 CGATTCTCCTGC
BfrI 887 CTTAAG C^TTAAG
6170 CTTAAG
BlnI 916 CCTAGG C^CTAGG
1934 CCTAGG
BstUI 1809 CGCG CG^CG
7530 CGCG
CfoI 131 GCGC GCG^C
138 GCGC
DraIII 3031 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
4248 CACTGTGTG
DrdI 4825 GACTCTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5627 GACCTCTCAGTC
EaeI 4178 TGGCCA [CT]^GGCC[GA]
5866 CGGCCA
EcoRV 2535 GATATC GAT^ATC
6285 GATATC
HhaI 131 GCGC GCG^C
138 GCGC
HinP1I 131 GCGC G^CGC
138 GCGC
HincII 299 GTCAAC GT[CT]^[GA]AC
5903 GTTAAC
HindII 299 GTCAAC GT[CT]^[GA]AC
5903 GTTAAC
MspA1I 2067 CAGCTG C[AC]G^C[GT]G
3156 CAGCTG
MvnI 1809 CGCG CG^CG
7530 CGCG
NheI 1051 GCTAGC G^CTAGC
3605 GCTAGC
PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT]
78 GGGTCCC
PshAI 409 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
734 GACACTGGTC
PvuII 2067 CAGCTG CAG^CTG
3156 CAGCTG
SacI 3275 GAGCTC GAGCT^C
4622 GAGCTC
SapI 5764 GCTCTTC GCTCTTC(1/4)
6073 GCTCTTC
ScaI 4118 AGTACT AGT^ACT
4203 AGTACT
SmaI 1654 CCCGGG CCC^GGG
2422 CCCGGG
TaqI 1781 TCGA T^CGA
6942 TCGA
TthHB8I 1781 TCGA T^CGA
6942 TCGA
XmaCI 1654 CCCGGG C^CCGGG
2422 CCCGGG
XmaI 1654 CCCGGG C^CCGGG
2422 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I Asp718I AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HindIII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XbaI XhoII XmnI