################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccIII AclI AcyI AgeI Aor51HI ApaI AscI AspI AviII BbeI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I CpoI DsaI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HgaI Hin1I KasI KspI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 7498 ACATGT A^C[GA][CT]GT AhdI 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1709 GTGCAC G^TGCAC ApaLI 1709 GTGCAC G^TGCAC AspEI 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 4178 TGGCCA TGG^CCA BbrPI 3034 CACGTG CAC^GTG BbvCI 2445 CCTCAGC CCTCAGC(-5/-2) BciVI 5352 GTATCC GTATCC(6/5) BclI 333 TGATCA T^GATCA BglI 6951 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 4624 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4624 GCTCAGC GC^T[ACGT]AGC BsaAI 3034 CACGTG [CT]AC^GT[GA] Bsp1407I 3823 TGTACA T^GTACA BspDI 6941 ATCGAT AT^CGAT BspHI 438 TCATGA T^CATGA BspLU11I 7498 ACATGT A^CATGT BsrGI 3823 TGTACA T^GTACA BstAPI 1641 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 4624 GCTCAGC GC^T[ACGT]AGC ClaI 6941 ATCGAT AT^CGAT Eam1105I 1582 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1161 CCTCCCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 6112 GAATTC G^AATTC EcoT22I 5816 ATGCAT ATGCA^T FbaI 333 TGATCA T^GATCA HpaI 5903 GTTAAC GTT^AAC MluNI 4178 TGGCCA TGG^CCA MscI 4178 TGGCCA TGG^CCA NsiI 5816 ATGCAT ATGCA^T PmaCI 3034 CACGTG CAC^GTG PmlI 3034 CACGTG CAC^GTG RcaI 438 TCATGA T^CATGA SexAI 3421 ACCTGGT A^CC[AT]GGT SpeI 5841 ACTAGT A^CTAGT SspBI 3823 TGTACA T^GTACA StuI 6958 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 152 GTCTAC GT^[AC][GT]AC 2861 GTCTAC AccII 1809 CGCG CG^CG 7530 CGCG AflII 887 CTTAAG C^TTAAG 6170 CTTAAG AvrII 916 CCTAGG C^CTAGG 1934 CCTAGG BcgI 1666 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2434 CGATTCTCCTGC BfrI 887 CTTAAG C^TTAAG 6170 CTTAAG BlnI 916 CCTAGG C^CTAGG 1934 CCTAGG BstUI 1809 CGCG CG^CG 7530 CGCG CfoI 131 GCGC GCG^C 138 GCGC DraIII 3031 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 4248 CACTGTGTG DrdI 4825 GACTCTATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5627 GACCTCTCAGTC EaeI 4178 TGGCCA [CT]^GGCC[GA] 5866 CGGCCA EcoRV 2535 GATATC GAT^ATC 6285 GATATC HhaI 131 GCGC GCG^C 138 GCGC HinP1I 131 GCGC G^CGC 138 GCGC HincII 299 GTCAAC GT[CT]^[GA]AC 5903 GTTAAC HindII 299 GTCAAC GT[CT]^[GA]AC 5903 GTTAAC MspA1I 2067 CAGCTG C[AC]G^C[GT]G 3156 CAGCTG MvnI 1809 CGCG CG^CG 7530 CGCG NheI 1051 GCTAGC G^CTAGC 3605 GCTAGC PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT] 78 GGGTCCC PshAI 409 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 734 GACACTGGTC PvuII 2067 CAGCTG CAG^CTG 3156 CAGCTG SacI 3275 GAGCTC GAGCT^C 4622 GAGCTC SapI 5764 GCTCTTC GCTCTTC(1/4) 6073 GCTCTTC ScaI 4118 AGTACT AGT^ACT 4203 AGTACT SmaI 1654 CCCGGG CCC^GGG 2422 CCCGGG TaqI 1781 TCGA T^CGA 6942 TCGA TthHB8I 1781 TCGA T^CGA 6942 TCGA XmaCI 1654 CCCGGG C^CCGGG 2422 CCCGGG XmaI 1654 CCCGGG C^CCGGG 2422 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I Asp718I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XhoII XmnI