##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccII AccIII Aor51HI AscI
Asp718I AspI AvaI BamHI BbeI
BbrPI BcgI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsoBI BspEI
BssHII BstBI BstUI CelII CpoI
DrdI EagI EclXI Eco47III Eco52I
FseI HindIII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmlI PvuI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I StuI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 26 GACGTC GACGT^C
AclI 3442 AACGTT AA^CGTT
AcyI 26 GACGTC G[GA]^CG[CT]C
AflIII 4686 ACATGT A^C[GA][CT]GT
AgeI 3403 ACCGGT A^CCGGT
AhdI 679 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 10 CCTGAGG CC^T[ACGT]AGG
ApaI 634 GGGCCC GGGCC^C
AspEI 679 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 6956 TGCGCA TGC^GCA
BglII 5429 AGATCT A^GATCT
BsaAI 2876 TACGTG [CT]AC^GT[GA]
BsaHI 26 GACGTC G[GA]^CG[CT]C
BsmI 7187 GAATGC GAATGC(1/-1)
BspDI 143 ATCGAT AT^CGAT
BspLU11I 4686 ACATGT A^CATGT
BsrBI 318 CCGCTC CCGCTC(-3/-3)
BssSI 7316 CACGAG CACGAG(-5/-1)
Bst1107I 7433 GTATAC GTA^TAC
BstZ17I 7433 GTATAC GTA^TAC
Bsu36I 10 CCTGAGG CC^T[ACGT]AGG
ClaI 143 ATCGAT AT^CGAT
Eam1105I 679 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 10 CCTGAGG CC^T[ACGT]AGG
FspI 6956 TGCGCA TGC^GCA
HaeII 4263 GGCGCT [GA]GCGC^[CT]
Hin1I 26 GACGTC G[GA]^CG[CT]C
MfeI 4739 CAATTG C^AATTG
MunI 4739 CAATTG C^AATTG
NdeI 7443 CATATG CA^TATG
NheI 5965 GCTAGC G^CTAGC
PflMI 3806 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3403 ACCGGT A^CCGGT
PmeI 4369 GTTTAAAC GTTT^AAAC
Psp1406I 3442 AACGTT AA^CGTT
PstI 1871 CTGCAG CTGCA^G
SacI 5884 GAGCTC GAGCT^C
SalI 6582 GTCGAC G^TCGAC
SexAI 917 ACCTGGT A^CC[AT]GGT
SwaI 1906 ATTTAAAT ATTT^AAAT
Van91I 3806 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1556 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 332 GTGCAC G^TGCAC
6257 GTGCAC
ApaLI 332 GTGCAC G^TGCAC
6257 GTGCAC
AseI 2157 ATTAAT AT^TAAT
2941 ATTAAT
AsnI 2157 ATTAAT AT^TAAT
2941 ATTAAT
Asp700I 4675 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7557 GAATTATTTC
AvrII 7390 CCTAGG C^CTAGG
7418 CCTAGG
BanI 5622 GGCACC G^G[CT][GA]CC
7040 GGTGCC
BglI 400 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
7097 GCCATGTTGGC
BlnI 7390 CCTAGG C^CTAGG
7418 CCTAGG
BsaWI 269 ACCGGA [AT]^CCGG[AT]
3403 ACCGGT
BsmBI 577 CGTCTC CGTCTC(1/5)
5893 CGTCTC
Bsp1407I 5211 TGTACA T^GTACA
6177 TGTACA
BsrGI 5211 TGTACA T^GTACA
6177 TGTACA
BstEII 499 GGTGACC G^GT[ACGT]ACC
4321 GGTAACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
4321 GGTAACC
DraIII 848 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5485 CACGGTGTG
DsaI 936 CCGTGG C^C[GA][CT]GG
6496 CCACGG
EcoNI 3837 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6134 CCTTAAGGAGG
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
4321 GGTAACC
EcoRV 2214 GATATC GAT^ATC
3555 GATATC
HincII 5522 GTTGAC GT[CT]^[GA]AC
6582 GTCGAC
HindII 5522 GTTGAC GT[CT]^[GA]AC
6582 GTCGAC
PpuMI 830 GGGTCCT [GA]G^G[AT]CC[CT]
2744 AGGTCCT
PshAI 91 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2562 GACAGGTGTC
PshBI 2157 ATTAAT AT^TAAT
2941 ATTAAT
ScaI 3119 AGTACT AGT^ACT
7353 AGTACT
SphI 446 GCATGC GCATG^C
6040 GCATGC
SspBI 5211 TGTACA T^GTACA
6177 TGTACA
SspI 2070 AATATT AAT^ATT
4929 AATATT
XmnI 4675 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7557 GAATTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SpeI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII