##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AclI AgeI AocI
Aor51HI AscI AspI AviII AvrII
BamHI BbvCI BcgI BciVI BclI
BfaI BglII BlnI BsaBI BsiWI
BsmBI BsmI BspDI BspHI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI Eco47III
Eco81I EcoO65I EcoT22I FbaI FseI
FspI HindIII HpaI KspI MaeI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2096 GGTACC G^GTACC
AccIII 495 TCCGGA T^CCGGA
AflII 2299 CTTAAG C^TTAAG
AflIII 2046 ACATGT A^C[GA][CT]GT
Alw44I 999 GTGCAC G^TGCAC
AlwNI 1128 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 999 GTGCAC G^TGCAC
AseI 144 ATTAAT AT^TAAT
AsnI 144 ATTAAT AT^TAAT
Asp700I 2025 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2096 GGTACC G^GTACC
BbrPI 963 CACGTG CAC^GTG
BfrI 2299 CTTAAG C^TTAAG
BpmI 367 CTGGAG CTGGAG(16/14)
BsaAI 963 CACGTG [CT]AC^GT[GA]
BsaWI 495 TCCGGA [AT]^CCGG[AT]
BseAI 495 TCCGGA T^CCGGA
BsgI 1049 CTGCAC GTGCAG(16/14)
BspEI 495 TCCGGA T^CCGGA
BspLU11I 2046 ACATGT A^CATGT
BsrDI 1031 GCAATG GCAATG(2/0)
BssHII 575 GCGCGC G^CGCGC
BstXI 359 CCACAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 922 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 172 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 353 CCGTGG C^C[GA][CT]GG
EcoNI 2183 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1878 GATATC GAT^ATC
EcoT14I 1854 CCAAGG C^C[AT][AT]GG
HincII 81 GTTGAC GT[CT]^[GA]AC
HindII 81 GTTGAC GT[CT]^[GA]AC
KpnI 2096 GGTACC GGTAC^C
MroI 495 TCCGGA T^CCGGA
NaeI 1044 GCCGGC GCC^GGC
NgoMIV 1044 GCCGGC G^CCGGC
PmaCI 963 CACGTG CAC^GTG
PmlI 963 CACGTG CAC^GTG
PpuMI 124 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2181 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 144 ATTAAT AT^TAAT
PstI 588 CTGCAG CTGCA^G
PvuI 1303 CGATCG CGAT^CG
PvuII 105 CAGCTG CAG^CTG
SexAI 2200 ACCTGGT A^CC[AT]GGT
SfcI 588 CTGCAG C^T[GA][CT]AG
SphI 1268 GCATGC GCATG^C
SspI 1831 AATATT AAT^ATT
StuI 654 AGGCCT AGG^CCT
StyI 1854 CCAAGG C^C[AT][AT]GG
TaqI 556 TCGA T^CGA
TfiI 1752 GAATC G^A[AT]TC
TthHB8I 556 TCGA T^CGA
XcmI 1005 CCACTTGGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2025 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AhdI 243 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1098 GACCCTGGGTC
ApaI 134 GGGCCC GGGCC^C
1180 GGGCCC
AspEI 243 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1098 GACCCTGGGTC
BalI 356 TGGCCA TGG^CCA
1035 TGGCCA
BlpI 849 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
Bpu1102I 849 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
BsaI 430 GAGACC GGTCTC(1/5)
990 GGTCTC
BsmAI 430 GAGAC GTCTC(1/5)
991 GTCTC
Bsp1407I 779 TGTACA T^GTACA
2272 TGTACA
BsrGI 779 TGTACA T^GTACA
2272 TGTACA
BstYI 31 GGATCT [GA]^GATC[CT]
1796 GGATCT
CelII 849 GCTGAGC GC^T[ACGT]AGC
1119 GCTGAGC
DraI 1583 TTTAAA TTT^AAA
2051 TTTAAA
EagI 324 CGGCCG C^GGCCG
1042 CGGCCG
Eam1105I 243 GACACTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1098 GACCCTGGGTC
EclXI 324 CGGCCG C^GGCCG
1042 CGGCCG
Eco52I 324 CGGCCG C^GGCCG
1042 CGGCCG
EcoRI 1625 GAATTC G^AATTC
1925 GAATTC
MflI 31 GGATCT [GA]^GATC[CT]
1796 GGATCT
MluNI 356 TGGCCA TGG^CCA
1035 TGGCCA
MscI 356 TGGCCA TGG^CCA
1035 TGGCCA
NspI 1268 GCATGC [GA]CATG^[CT]
2046 ACATGT
PflMI 921 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAGACAGTGG
SfaNI 1212 GATGC GCATC(5/9)
1947 GATGC
SmaI 624 CCCGGG CCC^GGG
1176 CCCGGG
SspBI 779 TGTACA T^GTACA
2272 TGTACA
Van91I 921 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1591 CCAGACAGTGG
XhoII 31 GGATCT [GA]^GATC[CT]
1796 GGATCT
XmaCI 624 CCCGGG C^CCGGG
1176 CCCGGG
XmaI 624 CCCGGG C^CCGGG
1176 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpuAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfoI SmlI
Tru9I TseI Tsp45I Tsp509I TspRI