##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII AvrII BamHI BbeI
BlnI BlpI Bpu1102I BsaHI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BsrBI BssHII BssSI BstBI
BstEII BstPI CelII ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HaeII
Hin1I KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI Sse8387I
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AspI 6573 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4994 CACGTG CAC^GTG
BcgI 2392 CGACTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1435 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2830 GGTCTC GGTCTC(1/5)
BsaWI 2998 ACCGGA [AT]^CCGG[AT]
BsgI 1279 GTGCAG GTGCAG(16/14)
Bsp1407I 4419 TGTACA T^GTACA
BsrFI 4159 ACCGGC [GA]^CCGG[CT]
BsrGI 4419 TGTACA T^GTACA
CfoI 7231 GCGC GCG^C
Cfr10I 4159 ACCGGC [GA]^CCGG[CT]
DraIII 6562 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2226 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 1967 GCGGG CCCGC(4/6)
HhaI 7231 GCGC GCG^C
HinP1I 7231 GCGC G^CGC
HpaI 1047 GTTAAC GTT^AAC
NdeI 6464 CATATG CA^TATG
PflFI 6573 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4994 CACGTG CAC^GTG
PmlI 4994 CACGTG CAC^GTG
PpuMI 2875 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 666 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2402 GCTCTTC GCTCTTC(1/4)
SexAI 3292 ACCTGGT A^CC[AT]GGT
SmaI 937 CCCGGG CCC^GGG
SnaBI 6373 TACGTA TAC^GTA
SpeI 1172 ACTAGT A^CTAGT
SphI 6341 GCATGC GCATG^C
SspBI 4419 TGTACA T^GTACA
StuI 5528 AGGCCT AGG^CCT
Tth111I 6573 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6484 TCTAGA T^CTAGA
XmaCI 937 CCCGGG C^CCGGG
XmaI 937 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1853 CGCG CG^CG
7230 CGCG
ApaI 4441 GGGCCC GGGCC^C
4527 GGGCCC
Asp700I 1260 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4550 GAAAGGATTC
BspMI 558 ACCTGC ACCTGC(4/8)
1470 ACCTGC
Bst1107I 350 GTATAC GTA^TAC
3762 GTATAC
BstAPI 4821 GCACTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5694 GCACTTTCTGC
BstUI 1853 CGCG CG^CG
7230 CGCG
BstZ17I 350 GTATAC GTA^TAC
3762 GTATAC
DsaI 397 CCATGG C^C[GA][CT]GG
958 CCATGG
EcoNI 699 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1863 CCTGCCGAAGG
EcoRV 1515 GATATC GAT^ATC
5726 GATATC
HgaI 613 GACGC GACGC(5/10)
2149 GACGC
MvnI 1853 CGCG CG^CG
7230 CGCG
NcoI 397 CCATGG C^CATGG
958 CCATGG
PaeR7I 2770 CTCGAG C^TCGAG
3913 CTCGAG
PvuII 1305 CAGCTG CAG^CTG
6254 CAGCTG
SacI 1846 GAGCTC GAGCT^C
2606 GAGCTC
SwaI 4902 ATTTAAAT ATTT^AAAT
6879 ATTTAAAT
XhoI 2770 CTCGAG C^TCGAG
3913 CTCGAG
XmnI 1260 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4550 GAAAGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AseI AsnI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaAI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspLU11I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII