##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspI AviII BbeI
BbrPI BcgI BciVI BsaHI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrDI BssHII Bst1107I BstBI BstUI
BstZ17I Bsu36I ClaI DrdI EagI
EclXI Eco47III Eco52I Eco81I FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MluI MroI MvnI
NarI NotI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PvuI SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 3871 GTAGAC GT^[AC][GT]AC
AflII 6197 CTTAAG C^TTAAG
AflIII 2009 ACATGT A^C[GA][CT]GT
AhdI 6522 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 6522 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfrI 6197 CTTAAG C^TTAAG
BsaAI 34 TACGTG [CT]AC^GT[GA]
BsaBI 2870 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 6529 CGTCTC CGTCTC(1/5)
Bsp1407I 880 TGTACA T^GTACA
BspLU11I 2009 ACATGT A^CATGT
BsrBI 277 CCGCTC CCGCTC(-3/-3)
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BsrGI 880 TGTACA T^GTACA
BssSI 6418 CACGAG CACGAG(-5/-1)
BstEII 4333 GGTTACC G^GT[ACGT]ACC
BstPI 4333 GGTTACC G^GT[ACGT]ACC
CfoI 3653 GCGC GCG^C
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
CpoI 140 CGGACCG CG^G[AT]CCG
Eam1105I 6522 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4333 GGTTACC G^GT[ACGT]ACC
EcoRV 2215 GATATC GAT^ATC
EcoT22I 2936 ATGCAT ATGCA^T
HhaI 3653 GCGC GCG^C
HinP1I 3653 GCGC G^CGC
HpaI 2015 GTTAAC GTT^AAC
MamI 2870 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5613 CAATTG C^AATTG
MunI 5613 CAATTG C^AATTG
NaeI 55 GCCGGC GCC^GGC
NdeI 3682 CATATG CA^TATG
NgoMIV 55 GCCGGC G^CCGGC
NheI 6284 GCTAGC G^CTAGC
NsiI 2936 ATGCAT ATGCA^T
PaeR7I 2745 CTCGAG C^TCGAG
PflMI 3640 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1341 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 5952 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 140 CGGACCG CG^G[AT]CCG
ScaI 5810 AGTACT AGT^ACT
SmaI 4710 CCCGGG CCC^GGG
SphI 5999 GCATGC GCATG^C
SspBI 880 TGTACA T^GTACA
Van91I 3640 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3449 CCAGTAACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2745 CTCGAG C^TCGAG
XmaCI 4710 CCCGGG C^CCGGG
XmaI 4710 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AclI 653 AACGTT AA^CGTT
3472 AACGTT
AvrII 3528 CCTAGG C^CTAGG
6603 CCTAGG
BlnI 3528 CCTAGG C^CTAGG
6603 CCTAGG
BlpI 3592 GCTCAGC GC^T[ACGT]AGC
3938 GCTAAGC
Bpu1102I 3592 GCTCAGC GC^T[ACGT]AGC
3938 GCTAAGC
CelII 3592 GCTCAGC GC^T[ACGT]AGC
3938 GCTAAGC
DraIII 1316 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
5942 CACAAAGTG
EcoNI 2221 CCTGTATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2866 CCTTGATGAGG
EcoRI 1143 GAATTC G^AATTC
6752 GAATTC
HincII 2015 GTTAAC GT[CT]^[GA]AC
5720 GTCAAC
HindII 2015 GTTAAC GT[CT]^[GA]AC
5720 GTCAAC
NcoI 503 CCATGG C^CATGG
2352 CCATGG
Psp1406I 653 AACGTT AA^CGTT
3472 AACGTT
StuI 224 AGGCCT AGG^CCT
5303 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AseI AsnI
Asp700I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI